Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtlD and mtlR overlap by 1 nucleotides mtlR and yibT are separated by 11 nucleotides yibT and yibL are separated by 284 nucleotides
b3600: mtlD - mannitol-1-phosphate 5-dehydrogenase (NCBI), at 3,772,447 to 3,773,595
mtlD
b3601: mtlR - DNA-binding repressor (NCBI), at 3,773,595 to 3,774,182
mtlR
b4554: yibT - hypothetical protein (NCBI), at 3,774,194 to 3,774,403
yibT
b3602: yibL - hypothetical protein (NCBI), at 3,774,688 to 3,775,050
yibL
Position (kb)
3773
3774
3775 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3772.614 kb on + strand, within mtlD at 3772.622 kb on - strand, within mtlD at 3772.679 kb on - strand, within mtlD at 3772.679 kb on - strand, within mtlD at 3772.714 kb on + strand, within mtlD at 3772.725 kb on - strand, within mtlD at 3772.725 kb on - strand, within mtlD at 3772.725 kb on - strand, within mtlD at 3772.774 kb on + strand, within mtlD at 3772.774 kb on + strand, within mtlD at 3772.782 kb on - strand, within mtlD at 3772.821 kb on + strand, within mtlD at 3772.823 kb on + strand, within mtlD at 3772.823 kb on + strand, within mtlD at 3772.823 kb on + strand, within mtlD at 3772.833 kb on + strand, within mtlD at 3772.833 kb on + strand, within mtlD at 3772.921 kb on - strand, within mtlD at 3772.935 kb on - strand, within mtlD at 3772.988 kb on + strand, within mtlD at 3772.988 kb on + strand, within mtlD at 3773.018 kb on - strand, within mtlD at 3773.032 kb on + strand, within mtlD at 3773.041 kb on - strand, within mtlD at 3773.051 kb on - strand, within mtlD at 3773.083 kb on + strand, within mtlD at 3773.114 kb on - strand, within mtlD at 3773.114 kb on - strand, within mtlD at 3773.114 kb on - strand, within mtlD at 3773.122 kb on - strand, within mtlD at 3773.150 kb on + strand, within mtlD at 3773.185 kb on + strand, within mtlD at 3773.185 kb on + strand, within mtlD at 3773.211 kb on - strand at 3773.211 kb on - strand, within mtlD at 3773.218 kb on + strand, within mtlD at 3773.226 kb on - strand, within mtlD at 3773.246 kb on - strand, within mtlD at 3773.246 kb on - strand, within mtlD at 3773.267 kb on - strand, within mtlD at 3773.267 kb on - strand, within mtlD at 3773.338 kb on - strand, within mtlD at 3773.351 kb on + strand, within mtlD at 3773.375 kb on - strand, within mtlD at 3773.427 kb on - strand, within mtlD at 3773.427 kb on - strand, within mtlD at 3773.448 kb on + strand, within mtlD at 3773.469 kb on + strand, within mtlD at 3773.477 kb on - strand, within mtlD at 3773.511 kb on - strand at 3773.562 kb on - strand at 3773.565 kb on + strand at 3773.568 kb on + strand at 3773.607 kb on + strand at 3773.609 kb on + strand at 3773.615 kb on - strand at 3773.627 kb on + strand at 3773.688 kb on + strand, within mtlR at 3773.688 kb on + strand, within mtlR at 3773.697 kb on + strand, within mtlR at 3773.718 kb on - strand, within mtlR at 3773.718 kb on - strand, within mtlR at 3773.810 kb on + strand, within mtlR at 3773.816 kb on - strand, within mtlR at 3773.818 kb on + strand, within mtlR at 3773.828 kb on - strand, within mtlR at 3773.828 kb on - strand, within mtlR at 3773.829 kb on + strand, within mtlR at 3773.857 kb on + strand, within mtlR at 3773.857 kb on + strand, within mtlR at 3773.928 kb on - strand, within mtlR at 3773.928 kb on - strand, within mtlR at 3774.003 kb on - strand, within mtlR at 3774.003 kb on - strand, within mtlR at 3774.007 kb on + strand, within mtlR at 3774.007 kb on + strand, within mtlR at 3774.012 kb on + strand, within mtlR at 3774.028 kb on - strand, within mtlR at 3774.028 kb on - strand, within mtlR at 3774.113 kb on - strand, within mtlR at 3774.123 kb on + strand, within mtlR at 3774.192 kb on + strand at 3774.192 kb on + strand at 3774.197 kb on + strand at 3774.197 kb on + strand at 3774.201 kb on + strand at 3774.201 kb on + strand at 3774.201 kb on + strand at 3774.201 kb on + strand at 3774.233 kb on + strand, within yibT at 3774.233 kb on + strand, within yibT at 3774.255 kb on - strand, within yibT at 3774.258 kb on - strand, within yibT at 3774.258 kb on - strand, within yibT at 3774.287 kb on - strand, within yibT at 3774.292 kb on - strand, within yibT at 3774.331 kb on - strand, within yibT at 3774.331 kb on - strand, within yibT at 3774.331 kb on - strand, within yibT at 3774.331 kb on - strand, within yibT at 3774.419 kb on - strand at 3774.424 kb on - strand at 3774.476 kb on - strand at 3774.490 kb on - strand at 3774.604 kb on - strand at 3774.622 kb on - strand at 3774.629 kb on + strand at 3774.629 kb on + strand at 3774.651 kb on - strand at 3774.651 kb on - strand at 3774.656 kb on - strand at 3774.656 kb on - strand at 3774.657 kb on - strand at 3774.732 kb on + strand, within yibL at 3774.738 kb on - strand, within yibL at 3774.738 kb on - strand, within yibL at 3774.781 kb on + strand, within yibL at 3774.858 kb on - strand, within yibL at 3774.867 kb on + strand, within yibL at 3774.898 kb on + strand, within yibL at 3774.898 kb on + strand, within yibL at 3774.938 kb on - strand, within yibL at 3774.938 kb on - strand, within yibL at 3774.944 kb on + strand, within yibL at 3774.944 kb on + strand, within yibL at 3774.973 kb on + strand, within yibL at 3774.994 kb on - strand, within yibL at 3775.062 kb on + strand at 3775.062 kb on + strand at 3775.125 kb on - strand at 3775.125 kb on - strand at 3775.130 kb on - strand at 3775.130 kb on - strand at 3775.156 kb on + strand at 3775.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 3,772,614 + mtlD b3600 0.15 -2.8 3,772,622 - mtlD b3600 0.15 -2.6 3,772,679 - mtlD b3600 0.20 -0.9 3,772,679 - mtlD b3600 0.20 +1.0 3,772,714 + mtlD b3600 0.23 -0.1 3,772,725 - mtlD b3600 0.24 -1.2 3,772,725 - mtlD b3600 0.24 -1.3 3,772,725 - mtlD b3600 0.24 -0.8 3,772,774 + mtlD b3600 0.28 +1.0 3,772,774 + mtlD b3600 0.28 -1.1 3,772,782 - mtlD b3600 0.29 +0.7 3,772,821 + mtlD b3600 0.33 -0.9 3,772,823 + mtlD b3600 0.33 +0.3 3,772,823 + mtlD b3600 0.33 +0.3 3,772,823 + mtlD b3600 0.33 +0.8 3,772,833 + mtlD b3600 0.34 -1.0 3,772,833 + mtlD b3600 0.34 -3.2 3,772,921 - mtlD b3600 0.41 -2.5 3,772,935 - mtlD b3600 0.42 +0.5 3,772,988 + mtlD b3600 0.47 -1.7 3,772,988 + mtlD b3600 0.47 -0.5 3,773,018 - mtlD b3600 0.50 +1.5 3,773,032 + mtlD b3600 0.51 -2.2 3,773,041 - mtlD b3600 0.52 -0.0 3,773,051 - mtlD b3600 0.53 +0.5 3,773,083 + mtlD b3600 0.55 -1.5 3,773,114 - mtlD b3600 0.58 -0.4 3,773,114 - mtlD b3600 0.58 -0.4 3,773,114 - mtlD b3600 0.58 +2.1 3,773,122 - mtlD b3600 0.59 -1.0 3,773,150 + mtlD b3600 0.61 -0.9 3,773,185 + mtlD b3600 0.64 -0.0 3,773,185 + mtlD b3600 0.64 -1.4 3,773,211 - +0.7 3,773,211 - mtlD b3600 0.66 +0.5 3,773,218 + mtlD b3600 0.67 +0.1 3,773,226 - mtlD b3600 0.68 -0.5 3,773,246 - mtlD b3600 0.70 +3.1 3,773,246 - mtlD b3600 0.70 +2.1 3,773,267 - mtlD b3600 0.71 -0.5 3,773,267 - mtlD b3600 0.71 +1.5 3,773,338 - mtlD b3600 0.78 -0.5 3,773,351 + mtlD b3600 0.79 -2.0 3,773,375 - mtlD b3600 0.81 -0.7 3,773,427 - mtlD b3600 0.85 -0.4 3,773,427 - mtlD b3600 0.85 -0.6 3,773,448 + mtlD b3600 0.87 +0.2 3,773,469 + mtlD b3600 0.89 +0.5 3,773,477 - mtlD b3600 0.90 -1.2 3,773,511 - -1.3 3,773,562 - -0.7 3,773,565 + +0.0 3,773,568 + +1.8 3,773,607 + -0.5 3,773,609 + -0.5 3,773,615 - -0.2 3,773,627 + +0.1 3,773,688 + mtlR b3601 0.16 -1.9 3,773,688 + mtlR b3601 0.16 -1.5 3,773,697 + mtlR b3601 0.17 -0.7 3,773,718 - mtlR b3601 0.21 +1.9 3,773,718 - mtlR b3601 0.21 -0.6 3,773,810 + mtlR b3601 0.37 -2.4 3,773,816 - mtlR b3601 0.38 +0.0 3,773,818 + mtlR b3601 0.38 +0.1 3,773,828 - mtlR b3601 0.40 -1.8 3,773,828 - mtlR b3601 0.40 -0.3 3,773,829 + mtlR b3601 0.40 +0.2 3,773,857 + mtlR b3601 0.45 -1.5 3,773,857 + mtlR b3601 0.45 +0.3 3,773,928 - mtlR b3601 0.57 -1.4 3,773,928 - mtlR b3601 0.57 -0.7 3,774,003 - mtlR b3601 0.69 -0.3 3,774,003 - mtlR b3601 0.69 -1.7 3,774,007 + mtlR b3601 0.70 +0.1 3,774,007 + mtlR b3601 0.70 +1.9 3,774,012 + mtlR b3601 0.71 -1.3 3,774,028 - mtlR b3601 0.74 -1.6 3,774,028 - mtlR b3601 0.74 +0.6 3,774,113 - mtlR b3601 0.88 -1.7 3,774,123 + mtlR b3601 0.90 -1.1 3,774,192 + +0.1 3,774,192 + +1.0 3,774,197 + -0.4 3,774,197 + +0.5 3,774,201 + -0.6 3,774,201 + +1.2 3,774,201 + -0.2 3,774,201 + -0.3 3,774,233 + yibT b4554 0.19 -1.5 3,774,233 + yibT b4554 0.19 -3.0 3,774,255 - yibT b4554 0.29 +0.0 3,774,258 - yibT b4554 0.30 -0.8 3,774,258 - yibT b4554 0.30 +0.7 3,774,287 - yibT b4554 0.44 +0.3 3,774,292 - yibT b4554 0.47 -0.4 3,774,331 - yibT b4554 0.65 -1.2 3,774,331 - yibT b4554 0.65 -0.7 3,774,331 - yibT b4554 0.65 -0.2 3,774,331 - yibT b4554 0.65 -2.3 3,774,419 - -3.1 3,774,424 - -0.6 3,774,476 - +0.9 3,774,490 - -1.9 3,774,604 - -3.3 3,774,622 - -2.2 3,774,629 + -0.4 3,774,629 + -1.6 3,774,651 - +0.2 3,774,651 - -0.7 3,774,656 - -0.2 3,774,656 - +0.4 3,774,657 - -2.5 3,774,732 + yibL b3602 0.12 +0.5 3,774,738 - yibL b3602 0.14 -1.1 3,774,738 - yibL b3602 0.14 -0.5 3,774,781 + yibL b3602 0.26 -1.1 3,774,858 - yibL b3602 0.47 -0.2 3,774,867 + yibL b3602 0.49 -0.3 3,774,898 + yibL b3602 0.58 +1.7 3,774,898 + yibL b3602 0.58 -1.6 3,774,938 - yibL b3602 0.69 -0.6 3,774,938 - yibL b3602 0.69 -1.1 3,774,944 + yibL b3602 0.71 +0.4 3,774,944 + yibL b3602 0.71 -0.5 3,774,973 + yibL b3602 0.79 -0.9 3,774,994 - yibL b3602 0.84 -0.3 3,775,062 + -1.0 3,775,062 + +0.8 3,775,125 - +0.3 3,775,125 - +0.3 3,775,130 - -1.8 3,775,130 - -2.4 3,775,156 + +2.5 3,775,156 + -0.1
Or see this region's nucleotide sequence