Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b3022 and ygiV are separated by 204 nucleotides ygiV and ygiW are separated by 52 nucleotides ygiW and qseB are separated by 151 nucleotides qseB and qseC overlap by 4 nucleotides
b3022: b3022 - predicted cyanide hydratase (NCBI), at 3,166,270 to 3,166,566
b3022
b3023: ygiV - predicted transcriptional regulator (NCBI), at 3,166,771 to 3,167,253
ygiV
b3024: ygiW - hypothetical protein (NCBI), at 3,167,306 to 3,167,698
ygiW
b3025: qseB - DNA-binding response regulator in two-component regulatory system with QseC (NCBI), at 3,167,850 to 3,168,509
qseB
b3026: qseC - sensory histidine kinase in two-component regulatory system with QseB (NCBI), at 3,168,506 to 3,169,855
qseC
Position (kb)
3167
3168 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3166.308 kb on - strand, within b3022 at 3166.308 kb on - strand, within b3022 at 3166.325 kb on - strand, within b3022 at 3166.330 kb on - strand, within b3022 at 3166.330 kb on - strand, within b3022 at 3166.335 kb on - strand, within b3022 at 3166.337 kb on + strand, within b3022 at 3166.345 kb on - strand, within b3022 at 3166.345 kb on - strand, within b3022 at 3166.346 kb on - strand, within b3022 at 3166.357 kb on - strand, within b3022 at 3166.370 kb on + strand, within b3022 at 3166.376 kb on - strand, within b3022 at 3166.377 kb on + strand, within b3022 at 3166.382 kb on + strand, within b3022 at 3166.382 kb on + strand, within b3022 at 3166.388 kb on - strand, within b3022 at 3166.388 kb on - strand, within b3022 at 3166.390 kb on - strand, within b3022 at 3166.420 kb on - strand, within b3022 at 3166.441 kb on - strand, within b3022 at 3166.480 kb on - strand, within b3022 at 3166.480 kb on - strand, within b3022 at 3166.523 kb on + strand, within b3022 at 3166.539 kb on + strand at 3166.551 kb on - strand at 3166.551 kb on - strand at 3166.551 kb on - strand at 3166.576 kb on - strand at 3166.576 kb on - strand at 3166.696 kb on - strand at 3166.703 kb on + strand at 3166.703 kb on + strand at 3166.771 kb on - strand at 3166.795 kb on - strand at 3166.795 kb on - strand at 3166.843 kb on + strand, within ygiV at 3166.909 kb on - strand, within ygiV at 3166.956 kb on - strand, within ygiV at 3166.956 kb on - strand, within ygiV at 3166.989 kb on + strand, within ygiV at 3166.989 kb on + strand, within ygiV at 3167.060 kb on + strand, within ygiV at 3167.154 kb on + strand, within ygiV at 3167.158 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.192 kb on + strand, within ygiV at 3167.227 kb on + strand at 3167.422 kb on - strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.478 kb on - strand, within ygiW at 3167.478 kb on - strand, within ygiW at 3167.500 kb on - strand, within ygiW at 3167.500 kb on - strand, within ygiW at 3167.512 kb on + strand, within ygiW at 3167.652 kb on + strand, within ygiW at 3167.718 kb on - strand at 3167.793 kb on - strand at 3167.793 kb on - strand at 3167.798 kb on + strand at 3167.800 kb on + strand at 3167.800 kb on + strand at 3167.800 kb on + strand at 3167.979 kb on + strand, within qseB at 3167.987 kb on - strand, within qseB at 3167.987 kb on - strand, within qseB at 3168.014 kb on - strand, within qseB at 3168.014 kb on - strand, within qseB at 3168.045 kb on + strand, within qseB at 3168.096 kb on + strand, within qseB at 3168.108 kb on + strand, within qseB at 3168.112 kb on + strand, within qseB at 3168.179 kb on - strand, within qseB at 3168.192 kb on + strand, within qseB at 3168.235 kb on + strand, within qseB at 3168.287 kb on + strand, within qseB at 3168.288 kb on + strand, within qseB at 3168.288 kb on + strand, within qseB at 3168.345 kb on + strand, within qseB at 3168.357 kb on - strand, within qseB at 3168.357 kb on - strand, within qseB at 3168.383 kb on + strand, within qseB at 3168.388 kb on - strand, within qseB at 3168.388 kb on - strand, within qseB at 3168.407 kb on + strand, within qseB at 3168.485 kb on + strand at 3168.499 kb on - strand at 3168.560 kb on - strand at 3168.678 kb on - strand, within qseC
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 3,166,308 - b3022 b3022 0.13 +0.3 3,166,308 - b3022 b3022 0.13 -1.4 3,166,325 - b3022 b3022 0.19 +0.2 3,166,330 - b3022 b3022 0.20 -1.3 3,166,330 - b3022 b3022 0.20 +0.8 3,166,335 - b3022 b3022 0.22 -1.9 3,166,337 + b3022 b3022 0.23 +2.0 3,166,345 - b3022 b3022 0.25 -0.1 3,166,345 - b3022 b3022 0.25 -1.7 3,166,346 - b3022 b3022 0.26 +0.4 3,166,357 - b3022 b3022 0.29 -0.4 3,166,370 + b3022 b3022 0.34 -0.7 3,166,376 - b3022 b3022 0.36 +0.1 3,166,377 + b3022 b3022 0.36 -0.3 3,166,382 + b3022 b3022 0.38 +0.7 3,166,382 + b3022 b3022 0.38 -0.1 3,166,388 - b3022 b3022 0.40 +0.2 3,166,388 - b3022 b3022 0.40 +0.2 3,166,390 - b3022 b3022 0.40 +0.2 3,166,420 - b3022 b3022 0.51 -2.6 3,166,441 - b3022 b3022 0.58 -0.8 3,166,480 - b3022 b3022 0.71 -0.2 3,166,480 - b3022 b3022 0.71 +1.0 3,166,523 + b3022 b3022 0.85 -0.5 3,166,539 + +0.1 3,166,551 - -1.3 3,166,551 - -0.4 3,166,551 - +0.6 3,166,576 - -1.3 3,166,576 - -0.9 3,166,696 - +0.6 3,166,703 + +0.2 3,166,703 + -1.3 3,166,771 - -0.9 3,166,795 - -0.9 3,166,795 - -0.4 3,166,843 + ygiV b3023 0.15 -0.2 3,166,909 - ygiV b3023 0.29 -0.2 3,166,956 - ygiV b3023 0.38 -2.0 3,166,956 - ygiV b3023 0.38 -0.5 3,166,989 + ygiV b3023 0.45 +0.8 3,166,989 + ygiV b3023 0.45 +0.2 3,167,060 + ygiV b3023 0.60 -1.1 3,167,154 + ygiV b3023 0.79 -0.9 3,167,158 + ygiV b3023 0.80 +0.9 3,167,175 + ygiV b3023 0.84 -0.7 3,167,175 + ygiV b3023 0.84 -1.3 3,167,175 + ygiV b3023 0.84 +1.8 3,167,192 + ygiV b3023 0.87 +0.3 3,167,227 + +0.5 3,167,422 - ygiW b3024 0.30 +0.0 3,167,470 + ygiW b3024 0.42 -1.4 3,167,470 + ygiW b3024 0.42 -1.9 3,167,470 + ygiW b3024 0.42 -0.4 3,167,478 - ygiW b3024 0.44 -2.7 3,167,478 - ygiW b3024 0.44 -1.7 3,167,500 - ygiW b3024 0.49 +0.3 3,167,500 - ygiW b3024 0.49 +0.2 3,167,512 + ygiW b3024 0.52 +0.4 3,167,652 + ygiW b3024 0.88 -0.2 3,167,718 - +0.1 3,167,793 - +0.2 3,167,793 - -0.2 3,167,798 + -0.2 3,167,800 + -1.9 3,167,800 + +0.6 3,167,800 + -1.9 3,167,979 + qseB b3025 0.20 -0.9 3,167,987 - qseB b3025 0.21 +0.3 3,167,987 - qseB b3025 0.21 +0.4 3,168,014 - qseB b3025 0.25 -0.8 3,168,014 - qseB b3025 0.25 -2.6 3,168,045 + qseB b3025 0.30 -0.3 3,168,096 + qseB b3025 0.37 +0.3 3,168,108 + qseB b3025 0.39 -1.4 3,168,112 + qseB b3025 0.40 -0.8 3,168,179 - qseB b3025 0.50 -0.1 3,168,192 + qseB b3025 0.52 -0.0 3,168,235 + qseB b3025 0.58 -0.3 3,168,287 + qseB b3025 0.66 -1.8 3,168,288 + qseB b3025 0.66 +0.4 3,168,288 + qseB b3025 0.66 -1.9 3,168,345 + qseB b3025 0.75 +0.0 3,168,357 - qseB b3025 0.77 -0.6 3,168,357 - qseB b3025 0.77 -0.8 3,168,383 + qseB b3025 0.81 +0.4 3,168,388 - qseB b3025 0.82 +2.0 3,168,388 - qseB b3025 0.82 +1.3 3,168,407 + qseB b3025 0.84 +1.0 3,168,485 + -0.4 3,168,499 - -0.5 3,168,560 - -1.0 3,168,678 - qseC b3026 0.13 +0.2
Or see this region's nucleotide sequence