Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutG and eutJ overlap by 11 nucleotides eutJ and eutE are separated by 10 nucleotides eutE and cchB are separated by 11 nucleotides cchB and cchA are separated by 106 nucleotides cchA and eutI are separated by 38 nucleotides
b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533
eutG
b2454: eutJ - predicted chaperonin, ethanolamine utilization protein (NCBI), at 2,567,523 to 2,568,359
eutJ
b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773
eutE
b2456: cchB - predicted carboxysome structural protein, ethanolamine utilization protein (NCBI), at 2,569,785 to 2,570,072
cchB
b2457: cchA - detox protein (VIMSS), at 2,570,179 to 2,570,472
cchA
b2458: eutI - predicted phosphotransacetylase subunit (NCBI), at 2,570,511 to 2,571,527
eutI
Position (kb)
2568
2569
2570 Strain fitness (log2 ratio)
-2
-1
0
1 at 2567.418 kb on - strand at 2567.595 kb on + strand at 2567.707 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.963 kb on + strand, within eutJ at 2568.118 kb on - strand, within eutJ at 2568.140 kb on - strand, within eutJ at 2568.149 kb on + strand, within eutJ at 2568.149 kb on + strand, within eutJ at 2568.219 kb on + strand, within eutJ at 2568.298 kb on + strand at 2568.338 kb on + strand at 2568.891 kb on - strand, within eutE at 2568.932 kb on + strand, within eutE at 2568.932 kb on + strand, within eutE at 2568.937 kb on + strand, within eutE at 2568.937 kb on + strand, within eutE at 2569.130 kb on + strand, within eutE at 2569.252 kb on - strand, within eutE at 2569.252 kb on - strand, within eutE at 2569.362 kb on + strand, within eutE at 2569.362 kb on + strand, within eutE at 2569.464 kb on - strand, within eutE at 2569.734 kb on + strand at 2569.820 kb on + strand, within cchB at 2569.837 kb on + strand, within cchB at 2569.849 kb on - strand, within cchB at 2569.849 kb on - strand, within cchB at 2570.117 kb on - strand at 2570.168 kb on + strand at 2570.168 kb on + strand at 2570.261 kb on - strand, within cchA at 2570.318 kb on - strand, within cchA at 2570.350 kb on + strand, within cchA at 2570.353 kb on - strand, within cchA at 2570.353 kb on - strand, within cchA at 2570.368 kb on - strand, within cchA at 2570.435 kb on + strand, within cchA at 2570.435 kb on + strand, within cchA at 2570.490 kb on - strand at 2570.502 kb on - strand at 2570.654 kb on - strand, within eutI
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 2,567,418 - +0.5 2,567,595 + +0.8 2,567,707 + eutJ b2454 0.22 +0.5 2,567,785 + eutJ b2454 0.31 -0.0 2,567,785 + eutJ b2454 0.31 -1.3 2,567,785 + eutJ b2454 0.31 +0.2 2,567,963 + eutJ b2454 0.53 -1.9 2,568,118 - eutJ b2454 0.71 -0.2 2,568,140 - eutJ b2454 0.74 +1.1 2,568,149 + eutJ b2454 0.75 -1.5 2,568,149 + eutJ b2454 0.75 +0.9 2,568,219 + eutJ b2454 0.83 +0.8 2,568,298 + -1.4 2,568,338 + +1.4 2,568,891 - eutE b2455 0.37 -0.2 2,568,932 + eutE b2455 0.40 -0.1 2,568,932 + eutE b2455 0.40 -2.3 2,568,937 + eutE b2455 0.40 +1.1 2,568,937 + eutE b2455 0.40 -1.5 2,569,130 + eutE b2455 0.54 -1.9 2,569,252 - eutE b2455 0.63 +1.5 2,569,252 - eutE b2455 0.63 -1.1 2,569,362 + eutE b2455 0.71 -2.2 2,569,362 + eutE b2455 0.71 -0.4 2,569,464 - eutE b2455 0.78 -0.3 2,569,734 + -1.3 2,569,820 + cchB b2456 0.12 +0.2 2,569,837 + cchB b2456 0.18 +0.1 2,569,849 - cchB b2456 0.22 -0.7 2,569,849 - cchB b2456 0.22 -0.7 2,570,117 - -0.6 2,570,168 + -1.4 2,570,168 + -0.3 2,570,261 - cchA b2457 0.28 -0.4 2,570,318 - cchA b2457 0.47 -1.5 2,570,350 + cchA b2457 0.58 -2.0 2,570,353 - cchA b2457 0.59 -0.1 2,570,353 - cchA b2457 0.59 -0.8 2,570,368 - cchA b2457 0.64 -1.0 2,570,435 + cchA b2457 0.87 +0.7 2,570,435 + cchA b2457 0.87 +0.7 2,570,490 - +0.5 2,570,502 - +0.5 2,570,654 - eutI b2458 0.14 -0.2
Or see this region's nucleotide sequence