Strain Fitness in Escherichia coli BW25113 around b2416

Experiment: TP4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysK and ptsH are separated by 383 nucleotidesptsH and ptsI are separated by 44 nucleotidesptsI and crr are separated by 40 nucleotidescrr and pdxK are separated by 42 nucleotides b2414: cysK - cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI), at 2,530,431 to 2,531,402 cysK b2415: ptsH - phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) (NCBI), at 2,531,786 to 2,532,043 ptsH b2416: ptsI - PEP-protein phosphotransferase of PTS system (enzyme I) (NCBI), at 2,532,088 to 2,533,815 ptsI b2417: crr - glucose-specific PTS system enzyme IIA component (NCBI), at 2,533,856 to 2,534,365 crr b2418: pdxK - pyridoxine kinase (NCBI), at 2,534,408 to 2,535,259 pdxK Position (kb) 2532 2533 2534Strain fitness (log2 ratio) -2 -1 0 1at 2531.138 kb on + strand, within cysKat 2531.138 kb on + strand, within cysKat 2531.169 kb on - strand, within cysKat 2531.314 kb on - strandat 2531.455 kb on + strandat 2531.455 kb on + strandat 2531.478 kb on + strandat 2531.478 kb on + strandat 2531.484 kb on + strandat 2531.666 kb on + strandat 2531.666 kb on + strandat 2531.725 kb on + strandat 2531.725 kb on + strandat 2534.053 kb on - strand, within crrat 2534.468 kb on - strandat 2534.488 kb on + strandat 2534.506 kb on - strand, within pdxKat 2534.519 kb on - strand, within pdxKat 2534.519 kb on - strand, within pdxKat 2534.591 kb on - strand, within pdxKat 2534.591 kb on - strand, within pdxKat 2534.601 kb on + strand, within pdxKat 2534.601 kb on + strand, within pdxKat 2534.618 kb on + strand, within pdxKat 2534.618 kb on + strand, within pdxKat 2534.708 kb on - strand, within pdxK

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Per-strain Table

Position Strand Gene LocusTag Fraction TP4
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2,531,138 + cysK b2414 0.73 +0.2
2,531,138 + cysK b2414 0.73 -1.3
2,531,169 - cysK b2414 0.76 -1.4
2,531,314 - +0.3
2,531,455 + -1.9
2,531,455 + -0.8
2,531,478 + -0.7
2,531,478 + +0.8
2,531,484 + -1.5
2,531,666 + -0.2
2,531,666 + +0.2
2,531,725 + +1.6
2,531,725 + -2.5
2,534,053 - crr b2417 0.39 -0.4
2,534,468 - -1.3
2,534,488 + +0.1
2,534,506 - pdxK b2418 0.12 -1.1
2,534,519 - pdxK b2418 0.13 +0.6
2,534,519 - pdxK b2418 0.13 +0.6
2,534,591 - pdxK b2418 0.21 +0.5
2,534,591 - pdxK b2418 0.21 -0.9
2,534,601 + pdxK b2418 0.23 +0.5
2,534,601 + pdxK b2418 0.23 -0.5
2,534,618 + pdxK b2418 0.25 -0.7
2,534,618 + pdxK b2418 0.25 -0.3
2,534,708 - pdxK b2418 0.35 -1.1

Or see this region's nucleotide sequence