Strain Fitness in Escherichia coli BW25113 around b1988

Experiment: TP4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcbl and nac are separated by 101 nucleotidesnac and asnV are separated by 326 nucleotidesasnV and erfK are separated by 55 nucleotides b1987: cbl - DNA-binding transcriptional activator of cysteine biosynthesis (NCBI), at 2,057,988 to 2,058,938 cbl b1988: nac - DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI), at 2,059,040 to 2,059,957 nac b1989: asnV - tRNA-Asn (NCBI), at 2,060,284 to 2,060,359 asnV b1990: erfK - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,060,415 to 2,061,347 erfK Position (kb) 2059 2060Strain fitness (log2 ratio) -3 -2 -1 0 1at 2058.044 kb on + strandat 2058.057 kb on - strandat 2058.057 kb on - strandat 2058.093 kb on + strand, within cblat 2058.094 kb on - strand, within cblat 2058.096 kb on - strand, within cblat 2058.096 kb on - strand, within cblat 2058.123 kb on + strand, within cblat 2058.218 kb on - strand, within cblat 2058.275 kb on + strand, within cblat 2058.575 kb on - strand, within cblat 2058.618 kb on + strand, within cblat 2058.644 kb on + strand, within cblat 2058.755 kb on + strand, within cblat 2058.755 kb on + strand, within cblat 2058.755 kb on + strand, within cblat 2058.813 kb on + strand, within cblat 2058.814 kb on + strand, within cblat 2058.891 kb on + strandat 2058.947 kb on - strandat 2059.036 kb on + strandat 2059.095 kb on - strandat 2059.095 kb on - strandat 2059.170 kb on + strand, within nacat 2059.188 kb on - strand, within nacat 2059.402 kb on + strand, within nacat 2059.487 kb on + strand, within nacat 2059.541 kb on - strand, within nacat 2059.584 kb on + strand, within nacat 2059.584 kb on + strand, within nacat 2059.643 kb on + strand, within nacat 2059.714 kb on - strand, within nacat 2059.896 kb on + strandat 2060.029 kb on + strandat 2060.165 kb on + strandat 2060.263 kb on - strandat 2060.346 kb on + strand, within asnVat 2060.520 kb on + strand, within erfKat 2060.520 kb on + strand, within erfKat 2060.530 kb on - strand, within erfKat 2060.536 kb on + strand, within erfKat 2060.573 kb on + strand, within erfKat 2060.610 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.715 kb on + strand, within erfKat 2060.730 kb on + strand, within erfKat 2060.730 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.821 kb on - strand, within erfK

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Per-strain Table

Position Strand Gene LocusTag Fraction TP4
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2,058,044 + -1.3
2,058,057 - -0.2
2,058,057 - -2.7
2,058,093 + cbl b1987 0.11 +1.1
2,058,094 - cbl b1987 0.11 -0.8
2,058,096 - cbl b1987 0.11 +0.8
2,058,096 - cbl b1987 0.11 -1.7
2,058,123 + cbl b1987 0.14 +0.3
2,058,218 - cbl b1987 0.24 -0.8
2,058,275 + cbl b1987 0.30 +1.7
2,058,575 - cbl b1987 0.62 +0.4
2,058,618 + cbl b1987 0.66 +1.5
2,058,644 + cbl b1987 0.69 -3.6
2,058,755 + cbl b1987 0.81 -0.6
2,058,755 + cbl b1987 0.81 -0.3
2,058,755 + cbl b1987 0.81 -1.8
2,058,813 + cbl b1987 0.87 +0.8
2,058,814 + cbl b1987 0.87 -0.2
2,058,891 + -2.5
2,058,947 - -0.0
2,059,036 + -1.5
2,059,095 - -3.0
2,059,095 - -0.1
2,059,170 + nac b1988 0.14 -1.0
2,059,188 - nac b1988 0.16 -1.7
2,059,402 + nac b1988 0.39 +0.1
2,059,487 + nac b1988 0.49 -0.7
2,059,541 - nac b1988 0.55 -1.9
2,059,584 + nac b1988 0.59 -1.5
2,059,584 + nac b1988 0.59 -2.4
2,059,643 + nac b1988 0.66 -0.9
2,059,714 - nac b1988 0.73 -0.0
2,059,896 + -1.9
2,060,029 + +1.1
2,060,165 + -0.8
2,060,263 - -2.1
2,060,346 + asnV b1989 0.82 +0.0
2,060,520 + erfK b1990 0.11 +0.4
2,060,520 + erfK b1990 0.11 -0.5
2,060,530 - erfK b1990 0.12 -0.2
2,060,536 + erfK b1990 0.13 +1.0
2,060,573 + erfK b1990 0.17 +0.4
2,060,610 - erfK b1990 0.21 -1.6
2,060,630 - erfK b1990 0.23 -0.5
2,060,630 - erfK b1990 0.23 -1.4
2,060,715 + erfK b1990 0.32 -0.3
2,060,730 + erfK b1990 0.34 +0.6
2,060,730 - erfK b1990 0.34 -0.5
2,060,743 - erfK b1990 0.35 -0.1
2,060,743 - erfK b1990 0.35 -1.3
2,060,821 - erfK b1990 0.44 -0.2

Or see this region's nucleotide sequence