Strain Fitness in Escherichia coli BW25113 around b1686

Experiment: TP4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsufA and rydB are separated by 326 nucleotidesrydB and ydiH are separated by 153 nucleotidesydiH and ydiI are separated by 99 nucleotidesydiI and ydiJ overlap by 4 nucleotides b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410 sufA b4430: rydB - regulatory sRNA (NCBI), at 1,762,737 to 1,762,804 rydB b1685: ydiH - predicted protein (RefSeq), at 1,762,958 to 1,763,146 ydiH b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656 ydiI b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709 ydiJ Position (kb) 1763 1764Strain fitness (log2 ratio) -2 -1 0 1at 1762.437 kb on + strandat 1762.521 kb on + strandat 1762.546 kb on - strandat 1762.891 kb on - strandat 1762.891 kb on - strandat 1762.904 kb on - strandat 1763.084 kb on - strand, within ydiHat 1763.150 kb on - strandat 1763.150 kb on - strandat 1763.186 kb on - strandat 1763.195 kb on + strandat 1763.195 kb on + strandat 1763.545 kb on + strand, within ydiIat 1763.577 kb on - strand, within ydiIat 1763.577 kb on - strand, within ydiIat 1763.692 kb on - strandat 1763.886 kb on - strandat 1764.308 kb on + strand, within ydiJat 1764.517 kb on + strand, within ydiJ

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Per-strain Table

Position Strand Gene LocusTag Fraction TP4
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1,762,437 + +1.2
1,762,521 + +0.6
1,762,546 - -1.2
1,762,891 - -1.3
1,762,891 - +1.2
1,762,904 - +1.0
1,763,084 - ydiH b1685 0.67 +0.8
1,763,150 - -1.7
1,763,150 - -0.3
1,763,186 - -0.9
1,763,195 + -2.0
1,763,195 + +1.2
1,763,545 + ydiI b1686 0.73 -1.8
1,763,577 - ydiI b1686 0.81 -0.3
1,763,577 - ydiI b1686 0.81 -2.1
1,763,692 - -0.9
1,763,886 - -0.5
1,764,308 + ydiJ b1687 0.21 +0.8
1,764,517 + ydiJ b1687 0.28 -0.0

Or see this region's nucleotide sequence