Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydfR and b1556 are separated by 4 nucleotides b1556 and cspB are separated by 753 nucleotides cspB and cspF are separated by 300 nucleotides cspF and b1559 are separated by 421 nucleotides
b1555: ydfR - Qin prophage; predicted protein (NCBI), at 1,638,078 to 1,638,389
ydfR
b1556: b1556 - orf, hypothetical protein (VIMSS), at 1,638,394 to 1,638,609
b1556
b1557: cspB - Qin prophage; cold shock protein (NCBI), at 1,639,363 to 1,639,578
cspB
b1558: cspF - Qin prophage; cold shock protein (NCBI), at 1,639,879 to 1,640,091
cspF
b1559: b1559 - orf, hypothetical protein (VIMSS), at 1,640,513 to 1,641,265
b1559
Position (kb)
1639
1640 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1638.405 kb on - strand at 1638.405 kb on - strand at 1638.487 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.495 kb on + strand, within b1556 at 1638.498 kb on - strand, within b1556 at 1638.513 kb on + strand, within b1556 at 1638.624 kb on + strand at 1638.624 kb on + strand at 1638.644 kb on - strand at 1638.653 kb on + strand at 1638.653 kb on + strand at 1638.658 kb on + strand at 1638.667 kb on + strand at 1638.667 kb on + strand at 1638.795 kb on + strand at 1638.800 kb on + strand at 1638.940 kb on + strand at 1638.988 kb on + strand at 1638.988 kb on + strand at 1638.988 kb on + strand at 1639.143 kb on - strand at 1639.143 kb on - strand at 1639.246 kb on - strand at 1639.298 kb on - strand at 1639.406 kb on - strand, within cspB at 1639.406 kb on - strand, within cspB at 1639.421 kb on - strand, within cspB at 1639.632 kb on - strand at 1639.632 kb on - strand at 1639.733 kb on - strand at 1639.753 kb on - strand at 1639.797 kb on + strand at 1639.806 kb on - strand at 1639.834 kb on - strand at 1640.045 kb on + strand, within cspF at 1640.045 kb on + strand, within cspF at 1640.069 kb on - strand, within cspF at 1640.110 kb on + strand at 1640.118 kb on - strand at 1640.124 kb on + strand at 1640.128 kb on + strand at 1640.132 kb on - strand at 1640.132 kb on - strand at 1640.132 kb on - strand at 1640.203 kb on + strand at 1640.204 kb on - strand at 1640.204 kb on - strand at 1640.204 kb on - strand at 1640.265 kb on + strand at 1640.350 kb on + strand at 1640.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 1,638,405 - +1.5 1,638,405 - +1.7 1,638,487 - b1556 b1556 0.43 +0.9 1,638,489 - b1556 b1556 0.44 -0.2 1,638,489 - b1556 b1556 0.44 +0.1 1,638,489 - b1556 b1556 0.44 -1.8 1,638,495 + b1556 b1556 0.47 +0.2 1,638,498 - b1556 b1556 0.48 -1.6 1,638,513 + b1556 b1556 0.55 +1.6 1,638,624 + +0.8 1,638,624 + +0.8 1,638,644 - +2.8 1,638,653 + +1.3 1,638,653 + -0.1 1,638,658 + +0.6 1,638,667 + +2.8 1,638,667 + +1.2 1,638,795 + +2.8 1,638,800 + +2.8 1,638,940 + +0.8 1,638,988 + +2.5 1,638,988 + +0.5 1,638,988 + -0.2 1,639,143 - +1.3 1,639,143 - -0.6 1,639,246 - +2.8 1,639,298 - -0.8 1,639,406 - cspB b1557 0.20 +1.3 1,639,406 - cspB b1557 0.20 -1.6 1,639,421 - cspB b1557 0.27 +1.1 1,639,632 - -0.8 1,639,632 - -0.1 1,639,733 - -1.3 1,639,753 - -2.0 1,639,797 + +2.8 1,639,806 - +1.2 1,639,834 - +2.8 1,640,045 + cspF b1558 0.78 +0.8 1,640,045 + cspF b1558 0.78 +1.8 1,640,069 - cspF b1558 0.89 +1.2 1,640,110 + +1.8 1,640,118 - +2.8 1,640,124 + +0.4 1,640,128 + -0.6 1,640,132 - -1.3 1,640,132 - -0.0 1,640,132 - +0.7 1,640,203 + -0.6 1,640,204 - +0.2 1,640,204 - +2.8 1,640,204 - +1.8 1,640,265 + -0.9 1,640,350 + -0.9 1,640,365 - +0.5
Or see this region's nucleotide sequence