Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uspE and fnr are separated by 151 nucleotides fnr and ogt are separated by 194 nucleotides ogt and ydaH are separated by 10 nucleotides
b1333: uspE - stress-induced protein (NCBI), at 1,395,696 to 1,396,646
uspE
b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550
fnr
b1335: ogt - O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI), at 1,397,745 to 1,398,260
ogt
b1336: ydaH - putative pump protein (transport) (VIMSS), at 1,398,271 to 1,399,797
ydaH
Position (kb)
1396
1397
1398 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1396.148 kb on + strand, within uspE at 1396.214 kb on - strand, within uspE at 1396.295 kb on - strand, within uspE at 1396.341 kb on + strand, within uspE at 1396.604 kb on + strand at 1396.609 kb on + strand at 1396.609 kb on + strand at 1396.728 kb on - strand at 1396.728 kb on - strand at 1397.086 kb on + strand, within fnr at 1397.086 kb on + strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.287 kb on - strand, within fnr at 1397.605 kb on + strand at 1397.605 kb on + strand at 1397.857 kb on - strand, within ogt at 1397.857 kb on - strand, within ogt at 1397.903 kb on + strand, within ogt at 1397.903 kb on + strand, within ogt at 1398.130 kb on + strand, within ogt at 1398.130 kb on + strand, within ogt at 1398.138 kb on + strand, within ogt at 1398.138 kb on + strand, within ogt
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 1,396,148 + uspE b1333 0.48 +1.0 1,396,214 - uspE b1333 0.54 -0.0 1,396,295 - uspE b1333 0.63 -3.4 1,396,341 + uspE b1333 0.68 +0.2 1,396,604 + +1.3 1,396,609 + +0.5 1,396,609 + -0.4 1,396,728 - +0.3 1,396,728 - -0.1 1,397,086 + fnr b1334 0.38 +1.2 1,397,086 + fnr b1334 0.38 -2.1 1,397,125 - fnr b1334 0.43 -0.1 1,397,125 - fnr b1334 0.43 -1.3 1,397,287 - fnr b1334 0.65 -1.9 1,397,605 + -0.2 1,397,605 + +0.1 1,397,857 - ogt b1335 0.22 +0.3 1,397,857 - ogt b1335 0.22 -1.4 1,397,903 + ogt b1335 0.31 +0.9 1,397,903 + ogt b1335 0.31 -0.8 1,398,130 + ogt b1335 0.75 +1.0 1,398,130 + ogt b1335 0.75 -0.8 1,398,138 + ogt b1335 0.76 +1.2 1,398,138 + ogt b1335 0.76 -1.6
Or see this region's nucleotide sequence