Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rne and b1085 are separated by 134 nucleotides b1085 and rluC are separated by 117 nucleotides
b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590
rne
b1085: b1085 - orf, hypothetical protein (VIMSS), at 1,143,725 to 1,144,045
b1085
b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122
rluC
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1143.725 kb on - strand at 1143.731 kb on - strand at 1143.821 kb on - strand, within b1085 at 1143.969 kb on - strand, within b1085 at 1143.969 kb on - strand, within b1085 at 1144.032 kb on - strand at 1144.039 kb on + strand at 1144.083 kb on + strand at 1144.109 kb on - strand at 1144.145 kb on - strand at 1144.295 kb on + strand, within rluC at 1144.438 kb on - strand, within rluC at 1144.509 kb on - strand, within rluC at 1144.575 kb on - strand, within rluC at 1144.592 kb on + strand, within rluC at 1144.610 kb on - strand, within rluC at 1144.655 kb on + strand, within rluC at 1144.655 kb on + strand, within rluC at 1144.699 kb on + strand, within rluC at 1144.699 kb on + strand, within rluC at 1144.867 kb on + strand, within rluC at 1144.867 kb on + strand, within rluC at 1144.899 kb on + strand, within rluC at 1144.988 kb on + strand, within rluC at 1144.988 kb on + strand, within rluC at 1144.998 kb on + strand, within rluC at 1144.998 kb on + strand, within rluC at 1145.011 kb on - strand, within rluC at 1145.011 kb on - strand, within rluC at 1145.035 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 1,143,725 - +0.9 1,143,731 - -0.1 1,143,821 - b1085 b1085 0.30 -2.7 1,143,969 - b1085 b1085 0.76 +0.6 1,143,969 - b1085 b1085 0.76 -2.4 1,144,032 - -0.1 1,144,039 + -0.4 1,144,083 + -0.5 1,144,109 - +0.4 1,144,145 - -2.3 1,144,295 + rluC b1086 0.14 -1.3 1,144,438 - rluC b1086 0.29 -1.3 1,144,509 - rluC b1086 0.36 -1.1 1,144,575 - rluC b1086 0.43 +1.1 1,144,592 + rluC b1086 0.45 -0.7 1,144,610 - rluC b1086 0.47 -3.0 1,144,655 + rluC b1086 0.51 -1.8 1,144,655 + rluC b1086 0.51 -1.9 1,144,699 + rluC b1086 0.56 -2.8 1,144,699 + rluC b1086 0.56 -1.6 1,144,867 + rluC b1086 0.73 -1.4 1,144,867 + rluC b1086 0.73 -0.9 1,144,899 + rluC b1086 0.77 -2.2 1,144,988 + rluC b1086 0.86 -2.3 1,144,988 + rluC b1086 0.86 -1.8 1,144,998 + rluC b1086 0.87 -1.7 1,144,998 + rluC b1086 0.87 +0.1 1,145,011 - rluC b1086 0.88 -0.8 1,145,011 - rluC b1086 0.88 -0.3 1,145,035 + -1.0
Or see this region's nucleotide sequence