Strain Fitness in Escherichia coli BW25113 around b0569

Experiment: TP4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfrA and nfrB overlap by 14 nucleotidesnfrB and cusS are separated by 149 nucleotides b0568: nfrA - bacteriophage N4 receptor, outer membrane subunit (NCBI), at 587,205 to 590,177 nfrA b0569: nfrB - bacteriophage N4 receptor, inner membrane subunit (NCBI), at 590,164 to 592,401 nfrB b0570: cusS - sensory histidine kinase in two-component regulatory system with CusR, senses copper ions (NCBI), at 592,551 to 593,993 cusS Position (kb) 590 591 592 593Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 589.179 kb on + strand, within nfrAat 589.200 kb on - strand, within nfrAat 589.255 kb on - strand, within nfrAat 589.452 kb on + strand, within nfrAat 589.523 kb on + strand, within nfrAat 589.546 kb on + strand, within nfrAat 589.546 kb on + strand, within nfrAat 589.656 kb on + strand, within nfrAat 589.780 kb on + strand, within nfrAat 589.789 kb on - strand, within nfrAat 589.829 kb on - strand, within nfrAat 589.857 kb on - strand, within nfrAat 589.908 kb on + strandat 589.908 kb on + strandat 589.919 kb on + strandat 589.928 kb on - strandat 589.928 kb on - strandat 589.961 kb on - strandat 589.964 kb on + strandat 589.977 kb on + strandat 590.006 kb on - strandat 590.010 kb on + strandat 590.010 kb on + strandat 590.071 kb on - strandat 590.071 kb on - strandat 590.071 kb on - strandat 590.088 kb on + strandat 590.099 kb on + strandat 590.142 kb on - strandat 590.143 kb on - strandat 590.163 kb on - strandat 590.163 kb on - strandat 590.195 kb on - strandat 590.195 kb on - strandat 590.202 kb on + strandat 590.202 kb on + strandat 590.217 kb on - strandat 590.232 kb on - strandat 590.247 kb on + strandat 590.309 kb on - strandat 590.344 kb on - strandat 590.344 kb on - strandat 590.438 kb on - strand, within nfrBat 590.474 kb on + strand, within nfrBat 590.482 kb on - strand, within nfrBat 590.789 kb on + strand, within nfrBat 590.789 kb on + strand, within nfrBat 590.872 kb on + strand, within nfrBat 590.872 kb on + strand, within nfrBat 590.885 kb on - strand, within nfrBat 590.885 kb on - strand, within nfrBat 591.089 kb on + strand, within nfrBat 591.089 kb on + strand, within nfrBat 591.188 kb on + strand, within nfrBat 591.188 kb on + strand, within nfrBat 591.188 kb on + strand, within nfrBat 591.189 kb on - strand, within nfrBat 591.257 kb on + strand, within nfrBat 591.257 kb on + strand, within nfrBat 591.318 kb on - strand, within nfrBat 591.318 kb on - strand, within nfrBat 591.343 kb on - strand, within nfrBat 591.343 kb on - strand, within nfrBat 591.705 kb on + strand, within nfrBat 591.750 kb on - strand, within nfrBat 591.750 kb on - strand, within nfrBat 591.767 kb on + strand, within nfrBat 591.808 kb on + strand, within nfrBat 591.888 kb on + strand, within nfrBat 592.009 kb on + strand, within nfrBat 592.011 kb on + strand, within nfrBat 592.021 kb on - strand, within nfrBat 592.025 kb on + strand, within nfrBat 592.168 kb on - strand, within nfrBat 592.179 kb on - strandat 592.205 kb on + strandat 592.205 kb on + strandat 592.565 kb on - strandat 592.565 kb on - strandat 592.696 kb on + strand, within cusSat 592.776 kb on + strand, within cusSat 592.776 kb on + strand, within cusSat 592.809 kb on - strand, within cusSat 592.822 kb on + strand, within cusSat 592.828 kb on - strand, within cusSat 592.828 kb on - strand, within cusSat 593.025 kb on - strand, within cusSat 593.031 kb on - strand, within cusSat 593.083 kb on + strand, within cusSat 593.091 kb on + strand, within cusSat 593.091 kb on + strand, within cusSat 593.248 kb on - strand, within cusSat 593.368 kb on + strand, within cusS

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Per-strain Table

Position Strand Gene LocusTag Fraction TP4
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589,179 + nfrA b0568 0.66 +1.9
589,200 - nfrA b0568 0.67 +0.2
589,255 - nfrA b0568 0.69 +1.0
589,452 + nfrA b0568 0.76 +0.7
589,523 + nfrA b0568 0.78 +0.4
589,546 + nfrA b0568 0.79 +0.1
589,546 + nfrA b0568 0.79 +1.1
589,656 + nfrA b0568 0.82 +0.6
589,780 + nfrA b0568 0.87 +0.6
589,789 - nfrA b0568 0.87 +0.6
589,829 - nfrA b0568 0.88 +0.8
589,857 - nfrA b0568 0.89 +0.9
589,908 + +0.0
589,908 + +1.6
589,919 + +0.9
589,928 - +2.6
589,928 - -0.9
589,961 - -0.4
589,964 + +1.0
589,977 + +1.5
590,006 - +0.4
590,010 + +1.3
590,010 + +0.7
590,071 - +1.3
590,071 - +0.7
590,071 - +0.4
590,088 + +1.5
590,099 + +2.2
590,142 - +1.1
590,143 - +1.1
590,163 - -0.8
590,163 - -0.7
590,195 - -1.5
590,195 - -0.0
590,202 + +0.8
590,202 + -2.1
590,217 - -1.6
590,232 - +0.6
590,247 + +2.3
590,309 - +0.6
590,344 - +0.3
590,344 - +0.7
590,438 - nfrB b0569 0.12 +1.2
590,474 + nfrB b0569 0.14 +0.9
590,482 - nfrB b0569 0.14 +1.6
590,789 + nfrB b0569 0.28 +1.0
590,789 + nfrB b0569 0.28 +2.0
590,872 + nfrB b0569 0.32 +0.9
590,872 + nfrB b0569 0.32 -2.3
590,885 - nfrB b0569 0.32 +1.1
590,885 - nfrB b0569 0.32 -0.5
591,089 + nfrB b0569 0.41 +0.7
591,089 + nfrB b0569 0.41 +1.6
591,188 + nfrB b0569 0.46 +1.6
591,188 + nfrB b0569 0.46 +1.0
591,188 + nfrB b0569 0.46 +1.6
591,189 - nfrB b0569 0.46 -1.0
591,257 + nfrB b0569 0.49 +0.1
591,257 + nfrB b0569 0.49 +1.3
591,318 - nfrB b0569 0.52 +1.1
591,318 - nfrB b0569 0.52 +1.5
591,343 - nfrB b0569 0.53 -1.1
591,343 - nfrB b0569 0.53 +0.4
591,705 + nfrB b0569 0.69 +0.6
591,750 - nfrB b0569 0.71 +0.6
591,750 - nfrB b0569 0.71 +0.3
591,767 + nfrB b0569 0.72 +1.6
591,808 + nfrB b0569 0.73 +1.3
591,888 + nfrB b0569 0.77 +0.3
592,009 + nfrB b0569 0.82 +0.2
592,011 + nfrB b0569 0.83 +1.0
592,021 - nfrB b0569 0.83 +0.2
592,025 + nfrB b0569 0.83 +0.8
592,168 - nfrB b0569 0.90 -0.2
592,179 - +2.7
592,205 + +0.9
592,205 + +0.5
592,565 - +0.2
592,565 - -1.0
592,696 + cusS b0570 0.10 -1.0
592,776 + cusS b0570 0.16 +0.5
592,776 + cusS b0570 0.16 +0.3
592,809 - cusS b0570 0.18 -0.2
592,822 + cusS b0570 0.19 +0.3
592,828 - cusS b0570 0.19 +0.4
592,828 - cusS b0570 0.19 +0.0
593,025 - cusS b0570 0.33 -0.4
593,031 - cusS b0570 0.33 -1.8
593,083 + cusS b0570 0.37 +0.3
593,091 + cusS b0570 0.37 +0.1
593,091 + cusS b0570 0.37 -3.0
593,248 - cusS b0570 0.48 +1.4
593,368 + cusS b0570 0.57 -2.2

Or see this region's nucleotide sequence