Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt betB and betI are separated by 13 nucleotides betI and betT are separated by 128 nucleotides betT and yahA are separated by 874 nucleotides
b0312: betB - betaine aldehyde dehydrogenase, NAD-dependent (NCBI), at 326,485 to 327,957
betB
b0313: betI - probably transcriptional repressor of bet genes (VIMSS), at 327,971 to 328,558
betI
b0314: betT - choline transporter of high affinity (NCBI), at 328,687 to 330,720
betT
b0315: yahA - predicted DNA-binding transcriptional regulator (NCBI), at 331,595 to 332,683
yahA
Position (kb)
328
329
330
331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 327.723 kb on + strand, within betB at 327.723 kb on + strand, within betB at 327.870 kb on - strand at 327.870 kb on - strand at 327.910 kb on + strand at 327.910 kb on + strand at 328.001 kb on - strand at 328.009 kb on - strand at 328.054 kb on - strand, within betI at 328.104 kb on + strand, within betI at 328.104 kb on + strand, within betI at 328.188 kb on + strand, within betI at 328.213 kb on - strand, within betI at 328.213 kb on - strand, within betI at 328.220 kb on - strand, within betI at 328.303 kb on + strand, within betI at 328.303 kb on + strand, within betI at 328.360 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.483 kb on - strand, within betI at 328.484 kb on - strand, within betI at 328.526 kb on + strand at 328.526 kb on + strand at 328.566 kb on + strand at 328.628 kb on - strand at 328.628 kb on - strand at 328.758 kb on + strand at 329.044 kb on + strand, within betT at 329.044 kb on + strand, within betT at 329.054 kb on - strand, within betT at 329.054 kb on - strand, within betT at 329.061 kb on + strand, within betT at 329.111 kb on - strand, within betT at 329.111 kb on - strand, within betT at 329.111 kb on - strand, within betT at 329.113 kb on + strand, within betT at 329.138 kb on + strand, within betT at 329.212 kb on - strand, within betT at 329.250 kb on + strand, within betT at 329.270 kb on + strand, within betT at 329.276 kb on - strand, within betT at 329.293 kb on + strand, within betT at 329.313 kb on + strand, within betT at 329.366 kb on - strand, within betT at 329.402 kb on - strand, within betT at 329.403 kb on - strand, within betT at 329.443 kb on - strand, within betT at 329.668 kb on - strand, within betT at 329.694 kb on + strand, within betT at 329.725 kb on - strand, within betT at 329.725 kb on - strand, within betT at 329.775 kb on + strand, within betT at 329.793 kb on + strand, within betT at 329.793 kb on + strand, within betT at 329.808 kb on + strand, within betT at 329.935 kb on - strand, within betT at 329.935 kb on - strand, within betT at 329.938 kb on - strand, within betT at 329.943 kb on + strand, within betT at 330.111 kb on + strand, within betT at 330.121 kb on + strand, within betT at 330.123 kb on + strand, within betT at 330.123 kb on + strand, within betT at 330.151 kb on - strand, within betT at 330.151 kb on - strand, within betT at 330.177 kb on - strand, within betT at 330.226 kb on + strand, within betT at 330.255 kb on - strand, within betT at 330.314 kb on - strand, within betT at 330.319 kb on - strand, within betT at 330.383 kb on + strand, within betT at 330.523 kb on - strand at 330.523 kb on - strand at 330.523 kb on - strand at 330.538 kb on + strand at 330.549 kb on + strand at 330.573 kb on + strand at 330.573 kb on + strand at 330.581 kb on - strand at 330.590 kb on + strand at 330.597 kb on + strand at 330.604 kb on + strand at 330.617 kb on - strand at 330.621 kb on + strand at 330.621 kb on + strand at 330.686 kb on + strand at 330.692 kb on + strand at 330.692 kb on + strand at 330.697 kb on + strand at 330.697 kb on + strand at 330.697 kb on + strand at 330.745 kb on + strand at 330.745 kb on + strand at 330.745 kb on + strand at 330.747 kb on + strand at 330.750 kb on + strand at 330.753 kb on - strand at 330.753 kb on - strand at 330.782 kb on + strand at 330.789 kb on + strand at 330.790 kb on + strand at 330.797 kb on + strand at 330.810 kb on - strand at 330.813 kb on + strand at 330.814 kb on + strand at 330.815 kb on + strand at 330.823 kb on + strand at 330.876 kb on + strand at 330.914 kb on + strand at 330.914 kb on + strand at 330.975 kb on - strand at 331.077 kb on - strand at 331.077 kb on - strand at 331.138 kb on + strand at 331.138 kb on + strand at 331.138 kb on + strand at 331.139 kb on + strand at 331.172 kb on + strand at 331.192 kb on + strand at 331.205 kb on + strand at 331.205 kb on + strand at 331.207 kb on + strand at 331.209 kb on + strand at 331.236 kb on + strand at 331.264 kb on + strand at 331.264 kb on + strand at 331.270 kb on + strand at 331.282 kb on + strand at 331.454 kb on - strand at 331.458 kb on + strand at 331.466 kb on - strand at 331.468 kb on - strand at 331.468 kb on - strand at 331.468 kb on - strand at 331.468 kb on - strand at 331.477 kb on - strand at 331.477 kb on - strand at 331.478 kb on - strand at 331.487 kb on + strand at 331.487 kb on + strand at 331.489 kb on + strand at 331.489 kb on + strand at 331.489 kb on - strand at 331.490 kb on - strand at 331.492 kb on - strand at 331.496 kb on + strand at 331.496 kb on - strand at 331.496 kb on - strand at 331.497 kb on - strand at 331.497 kb on - strand at 331.497 kb on - strand at 331.497 kb on - strand at 331.497 kb on - strand at 331.504 kb on - strand at 331.504 kb on - strand at 331.512 kb on - strand at 331.519 kb on - strand at 331.520 kb on - strand at 331.520 kb on - strand at 331.523 kb on + strand at 331.525 kb on + strand at 331.525 kb on + strand at 331.525 kb on - strand at 331.525 kb on - strand at 331.525 kb on - strand at 331.525 kb on - strand at 331.527 kb on - strand at 331.531 kb on - strand at 331.531 kb on - strand at 331.533 kb on - strand at 331.533 kb on - strand at 331.533 kb on - strand at 331.533 kb on - strand at 331.534 kb on - strand at 331.534 kb on - strand at 331.536 kb on - strand at 331.540 kb on + strand at 331.540 kb on + strand at 331.540 kb on + strand at 331.540 kb on + strand at 331.541 kb on - strand at 331.541 kb on - strand at 331.542 kb on + strand at 331.550 kb on + strand at 331.550 kb on + strand at 331.551 kb on + strand at 331.556 kb on + strand at 331.558 kb on + strand at 331.558 kb on - strand at 331.558 kb on - strand at 331.564 kb on - strand at 331.564 kb on - strand at 331.566 kb on - strand at 331.566 kb on - strand at 331.582 kb on - strand at 331.582 kb on - strand at 331.628 kb on - strand at 331.628 kb on - strand at 331.648 kb on - strand at 331.648 kb on - strand at 331.648 kb on - strand at 331.660 kb on + strand at 331.661 kb on + strand at 331.662 kb on + strand at 331.665 kb on - strand at 331.666 kb on - strand at 331.681 kb on - strand at 331.685 kb on + strand at 331.695 kb on + strand at 331.695 kb on + strand at 331.696 kb on - strand at 331.696 kb on - strand at 331.696 kb on - strand at 331.697 kb on - strand at 331.698 kb on - strand at 331.698 kb on - strand at 331.702 kb on + strand at 331.710 kb on + strand, within yahA
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 327,723 + betB b0312 0.84 -1.4 327,723 + betB b0312 0.84 -2.4 327,870 - +2.0 327,870 - -1.2 327,910 + +0.3 327,910 + -1.3 328,001 - -0.0 328,009 - -2.2 328,054 - betI b0313 0.14 +0.0 328,104 + betI b0313 0.23 -0.5 328,104 + betI b0313 0.23 -0.5 328,188 + betI b0313 0.37 -0.8 328,213 - betI b0313 0.41 +0.2 328,213 - betI b0313 0.41 +1.7 328,220 - betI b0313 0.42 +0.1 328,303 + betI b0313 0.56 -2.3 328,303 + betI b0313 0.56 -0.4 328,360 + betI b0313 0.66 +0.4 328,475 + betI b0313 0.86 +0.4 328,475 + betI b0313 0.86 -0.8 328,483 - betI b0313 0.87 -1.1 328,484 - betI b0313 0.87 -0.5 328,526 + +0.4 328,526 + -2.6 328,566 + +0.4 328,628 - +0.8 328,628 - -1.2 328,758 + +0.0 329,044 + betT b0314 0.18 -2.8 329,044 + betT b0314 0.18 +0.8 329,054 - betT b0314 0.18 +1.5 329,054 - betT b0314 0.18 -0.4 329,061 + betT b0314 0.18 +0.5 329,111 - betT b0314 0.21 -0.9 329,111 - betT b0314 0.21 +0.5 329,111 - betT b0314 0.21 +1.3 329,113 + betT b0314 0.21 +0.7 329,138 + betT b0314 0.22 +0.9 329,212 - betT b0314 0.26 -3.2 329,250 + betT b0314 0.28 -0.9 329,270 + betT b0314 0.29 -0.9 329,276 - betT b0314 0.29 +0.5 329,293 + betT b0314 0.30 +0.3 329,313 + betT b0314 0.31 +0.8 329,366 - betT b0314 0.33 +0.1 329,402 - betT b0314 0.35 -1.2 329,403 - betT b0314 0.35 -0.2 329,443 - betT b0314 0.37 +0.7 329,668 - betT b0314 0.48 -0.8 329,694 + betT b0314 0.50 +0.3 329,725 - betT b0314 0.51 +2.8 329,725 - betT b0314 0.51 +1.5 329,775 + betT b0314 0.53 +0.2 329,793 + betT b0314 0.54 +2.6 329,793 + betT b0314 0.54 -0.4 329,808 + betT b0314 0.55 +0.9 329,935 - betT b0314 0.61 -1.7 329,935 - betT b0314 0.61 +1.3 329,938 - betT b0314 0.62 +0.0 329,943 + betT b0314 0.62 -0.1 330,111 + betT b0314 0.70 -2.8 330,121 + betT b0314 0.71 +0.0 330,123 + betT b0314 0.71 +1.7 330,123 + betT b0314 0.71 +1.4 330,151 - betT b0314 0.72 +1.9 330,151 - betT b0314 0.72 +0.9 330,177 - betT b0314 0.73 +0.0 330,226 + betT b0314 0.76 -0.9 330,255 - betT b0314 0.77 -0.6 330,314 - betT b0314 0.80 -0.3 330,319 - betT b0314 0.80 -0.1 330,383 + betT b0314 0.83 -0.8 330,523 - -1.1 330,523 - -0.4 330,523 - +0.3 330,538 + -1.2 330,549 + +0.4 330,573 + +0.3 330,573 + +1.4 330,581 - +1.0 330,590 + +0.0 330,597 + +0.7 330,604 + +0.1 330,617 - +2.0 330,621 + -1.2 330,621 + -0.2 330,686 + -0.4 330,692 + +0.2 330,692 + -2.6 330,697 + +1.3 330,697 + -0.6 330,697 + +0.7 330,745 + -1.6 330,745 + -1.2 330,745 + +0.8 330,747 + -1.8 330,750 + +0.8 330,753 - +3.0 330,753 - +3.0 330,782 + -1.2 330,789 + -0.7 330,790 + -0.9 330,797 + -0.5 330,810 - +1.0 330,813 + +0.5 330,814 + +1.0 330,815 + -1.4 330,823 + -0.2 330,876 + -0.8 330,914 + -0.8 330,914 + -1.6 330,975 - +0.5 331,077 - +0.4 331,077 - +0.6 331,138 + +3.0 331,138 + +3.0 331,138 + +3.0 331,139 + +3.0 331,172 + +2.0 331,192 + +3.0 331,205 + +3.0 331,205 + +1.6 331,207 + -0.1 331,209 + +0.6 331,236 + +3.0 331,264 + +1.2 331,264 + -0.2 331,270 + -2.6 331,282 + +0.1 331,454 - +0.2 331,458 + +0.4 331,466 - -0.2 331,468 - -0.6 331,468 - -0.2 331,468 - -1.7 331,468 - -1.9 331,477 - +1.6 331,477 - +1.0 331,478 - +0.4 331,487 + +2.0 331,487 + -0.6 331,489 + +1.0 331,489 + +0.2 331,489 - +0.6 331,490 - -1.1 331,492 - +3.0 331,496 + +1.4 331,496 - -1.0 331,496 - -0.5 331,497 - +0.2 331,497 - +0.4 331,497 - +1.0 331,497 - +1.4 331,497 - -1.5 331,504 - +0.3 331,504 - +1.0 331,512 - -0.5 331,519 - -1.7 331,520 - +1.6 331,520 - +2.0 331,523 + -1.4 331,525 + +0.3 331,525 + +0.8 331,525 - -0.9 331,525 - +2.0 331,525 - +1.0 331,525 - +1.4 331,527 - +1.4 331,531 - +0.2 331,531 - +1.7 331,533 - +0.5 331,533 - -0.6 331,533 - -0.9 331,533 - -0.7 331,534 - +0.9 331,534 - -0.0 331,536 - +0.7 331,540 + -0.4 331,540 + -0.6 331,540 + +0.2 331,540 + +1.3 331,541 - +0.6 331,541 - +2.0 331,542 + -0.9 331,550 + +1.0 331,550 + +1.0 331,551 + +1.9 331,556 + +1.6 331,558 + -1.6 331,558 - +2.4 331,558 - -0.2 331,564 - -1.0 331,564 - -1.8 331,566 - +2.5 331,566 - +0.6 331,582 - +0.4 331,582 - +2.0 331,628 - +1.7 331,628 - -1.0 331,648 - -1.0 331,648 - -0.0 331,648 - -1.0 331,660 + -0.4 331,661 + +1.7 331,662 + -0.8 331,665 - +1.4 331,666 - -0.3 331,681 - +1.6 331,685 + -0.1 331,695 + -1.4 331,695 + +1.1 331,696 - +1.0 331,696 - +1.2 331,696 - -0.5 331,697 - +0.8 331,698 - +0.4 331,698 - +0.6 331,702 + -2.1 331,710 + yahA b0315 0.11 +1.0
Or see this region's nucleotide sequence