Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadN and folK are separated by 369 nucleotides folK and pcnB overlap by 4 nucleotides pcnB and yadB are separated by 59 nucleotides
b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883
yadN
b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732
folK
b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126
pcnB
b0144: yadB - glutamyl-Q tRNA(Asp) synthetase (RefSeq), at 159,186 to 160,112
yadB
Position (kb)
157
158
159
160 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 156.734 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.744 kb on + strand, within yadN at 156.755 kb on + strand, within yadN at 156.757 kb on + strand, within yadN at 156.759 kb on + strand, within yadN at 156.786 kb on + strand, within yadN at 156.789 kb on + strand, within yadN at 156.793 kb on + strand, within yadN at 156.804 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.825 kb on + strand at 156.827 kb on + strand at 156.827 kb on + strand at 156.834 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.850 kb on + strand at 156.854 kb on + strand at 156.854 kb on + strand at 157.218 kb on - strand at 157.279 kb on - strand at 157.291 kb on - strand at 157.301 kb on - strand, within folK at 157.745 kb on + strand at 157.870 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.923 kb on - strand, within pcnB at 158.143 kb on + strand, within pcnB at 158.156 kb on + strand, within pcnB at 158.227 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.312 kb on - strand, within pcnB at 158.342 kb on - strand, within pcnB at 158.378 kb on - strand, within pcnB at 158.400 kb on + strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.426 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.447 kb on - strand, within pcnB at 158.516 kb on + strand, within pcnB at 158.522 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.864 kb on - strand, within pcnB at 159.038 kb on - strand at 159.093 kb on - strand at 159.093 kb on - strand at 159.133 kb on + strand at 159.347 kb on - strand, within yadB at 159.347 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.581 kb on - strand, within yadB at 159.655 kb on + strand, within yadB at 159.699 kb on + strand, within yadB at 159.722 kb on - strand, within yadB at 159.722 kb on - strand, within yadB at 159.743 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.770 kb on + strand, within yadB at 159.770 kb on + strand, within yadB at 159.803 kb on + strand, within yadB at 159.808 kb on + strand, within yadB at 159.905 kb on - strand, within yadB at 159.947 kb on + strand, within yadB at 160.046 kb on + strand at 160.068 kb on + strand at 160.068 kb on - strand at 160.068 kb on - strand at 160.070 kb on - strand at 160.078 kb on + strand at 160.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 156,734 + yadN b0141 0.74 -0.7 156,735 + yadN b0141 0.75 +0.7 156,735 + yadN b0141 0.75 -0.4 156,744 + yadN b0141 0.76 +0.2 156,755 + yadN b0141 0.78 -1.6 156,757 + yadN b0141 0.78 +2.4 156,759 + yadN b0141 0.79 -0.7 156,786 + yadN b0141 0.83 +3.0 156,789 + yadN b0141 0.84 -0.9 156,793 + yadN b0141 0.84 -0.9 156,804 + yadN b0141 0.86 -1.1 156,823 + yadN b0141 0.90 +0.7 156,823 + yadN b0141 0.90 +0.0 156,825 + -2.0 156,827 + +1.3 156,827 + +1.3 156,834 + +3.4 156,849 + +0.7 156,849 + -1.2 156,849 + +0.0 156,850 + -1.1 156,854 + +1.0 156,854 + +0.0 157,218 - +1.0 157,279 - -0.8 157,291 - +0.4 157,301 - folK b0142 0.10 -0.7 157,745 + +0.3 157,870 - pcnB b0143 0.10 -1.3 157,906 - pcnB b0143 0.13 -0.9 157,906 - pcnB b0143 0.13 -0.6 157,923 - pcnB b0143 0.14 -0.3 158,143 + pcnB b0143 0.30 -0.1 158,156 + pcnB b0143 0.31 -2.0 158,227 - pcnB b0143 0.36 -2.2 158,249 - pcnB b0143 0.37 -0.5 158,249 - pcnB b0143 0.37 -2.7 158,312 - pcnB b0143 0.42 -1.7 158,342 - pcnB b0143 0.44 -0.1 158,378 - pcnB b0143 0.46 -0.7 158,400 + pcnB b0143 0.48 -1.6 158,408 - pcnB b0143 0.49 -1.6 158,408 - pcnB b0143 0.49 -2.1 158,426 - pcnB b0143 0.50 -1.4 158,442 - pcnB b0143 0.51 -1.0 158,442 - pcnB b0143 0.51 +1.8 158,447 - pcnB b0143 0.51 -0.1 158,516 + pcnB b0143 0.56 +0.3 158,522 - pcnB b0143 0.57 +0.5 158,759 - pcnB b0143 0.74 -2.0 158,759 - pcnB b0143 0.74 +0.2 158,759 - pcnB b0143 0.74 +0.3 158,864 - pcnB b0143 0.81 -1.5 159,038 - -3.4 159,093 - +0.0 159,093 - -3.2 159,133 + -0.3 159,347 - yadB b0144 0.17 +0.7 159,347 - yadB b0144 0.17 +0.0 159,352 - yadB b0144 0.18 +1.1 159,352 - yadB b0144 0.18 +0.6 159,581 - yadB b0144 0.43 -0.2 159,655 + yadB b0144 0.51 -0.5 159,699 + yadB b0144 0.55 +1.0 159,722 - yadB b0144 0.58 +0.1 159,722 - yadB b0144 0.58 +0.1 159,743 - yadB b0144 0.60 -0.1 159,754 - yadB b0144 0.61 -0.3 159,754 - yadB b0144 0.61 -0.8 159,770 + yadB b0144 0.63 +0.6 159,770 + yadB b0144 0.63 +0.9 159,803 + yadB b0144 0.67 -0.4 159,808 + yadB b0144 0.67 -2.1 159,905 - yadB b0144 0.78 +0.7 159,947 + yadB b0144 0.82 +0.1 160,046 + +0.2 160,068 + +1.0 160,068 - -0.4 160,068 - -2.8 160,070 - +0.7 160,078 + -0.0 160,078 + +0.1
Or see this region's nucleotide sequence