Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metE and ysgA are separated by 39 nucleotides ysgA and udp are separated by 261 nucleotides
b3829: metE - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI), at 4,011,076 to 4,013,337
metE
b3830: ysgA - predicted hydrolase (NCBI), at 4,013,377 to 4,014,192
ysgA
b3831: udp - uridine phosphorylase (NCBI), at 4,014,454 to 4,015,215
udp
Position (kb)
4013
4014
4015 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4012.402 kb on - strand, within metE at 4012.466 kb on + strand, within metE at 4012.467 kb on + strand, within metE at 4012.474 kb on - strand, within metE at 4012.511 kb on + strand, within metE at 4012.511 kb on + strand, within metE at 4012.595 kb on - strand, within metE at 4012.600 kb on + strand, within metE at 4012.600 kb on + strand, within metE at 4012.606 kb on - strand, within metE at 4012.613 kb on - strand, within metE at 4012.613 kb on - strand, within metE at 4012.615 kb on - strand, within metE at 4012.663 kb on + strand, within metE at 4012.672 kb on + strand, within metE at 4012.672 kb on + strand, within metE at 4012.731 kb on + strand, within metE at 4012.731 kb on + strand, within metE at 4012.738 kb on - strand, within metE at 4012.738 kb on - strand, within metE at 4012.750 kb on - strand, within metE at 4012.820 kb on + strand, within metE at 4012.835 kb on + strand, within metE at 4012.861 kb on + strand, within metE at 4012.861 kb on + strand, within metE at 4012.866 kb on + strand, within metE at 4012.866 kb on + strand, within metE at 4012.935 kb on - strand, within metE at 4012.935 kb on - strand, within metE at 4013.004 kb on - strand, within metE at 4013.048 kb on + strand, within metE at 4013.048 kb on + strand, within metE at 4013.063 kb on - strand, within metE at 4013.135 kb on + strand at 4013.135 kb on + strand at 4013.183 kb on + strand at 4013.192 kb on - strand at 4013.192 kb on - strand at 4013.193 kb on - strand at 4013.193 kb on - strand at 4013.224 kb on + strand at 4013.235 kb on - strand at 4013.235 kb on - strand at 4013.250 kb on - strand at 4013.250 kb on - strand at 4013.250 kb on - strand at 4013.250 kb on - strand at 4013.359 kb on - strand at 4013.359 kb on - strand at 4013.359 kb on - strand at 4013.393 kb on + strand at 4013.401 kb on - strand at 4013.403 kb on + strand at 4013.406 kb on - strand at 4013.417 kb on + strand at 4013.502 kb on + strand, within ysgA at 4013.531 kb on - strand, within ysgA at 4013.540 kb on + strand, within ysgA at 4013.540 kb on + strand, within ysgA at 4013.560 kb on - strand, within ysgA at 4013.678 kb on + strand, within ysgA at 4013.723 kb on + strand, within ysgA at 4013.723 kb on + strand, within ysgA at 4013.735 kb on + strand, within ysgA at 4013.735 kb on + strand, within ysgA at 4013.736 kb on - strand, within ysgA at 4013.737 kb on + strand, within ysgA at 4013.742 kb on + strand, within ysgA at 4013.742 kb on + strand, within ysgA at 4013.747 kb on + strand, within ysgA at 4013.781 kb on - strand, within ysgA at 4013.822 kb on + strand, within ysgA at 4013.858 kb on - strand, within ysgA at 4013.860 kb on - strand, within ysgA at 4013.872 kb on - strand, within ysgA at 4013.952 kb on + strand, within ysgA at 4014.006 kb on + strand, within ysgA at 4014.030 kb on + strand, within ysgA at 4014.045 kb on - strand, within ysgA at 4014.056 kb on - strand, within ysgA at 4014.066 kb on - strand, within ysgA at 4014.066 kb on - strand, within ysgA at 4014.084 kb on - strand, within ysgA at 4014.084 kb on - strand, within ysgA at 4014.086 kb on - strand, within ysgA at 4014.124 kb on + strand at 4014.124 kb on + strand at 4014.183 kb on + strand at 4014.194 kb on + strand at 4014.198 kb on + strand at 4014.260 kb on - strand at 4014.260 kb on - strand at 4014.296 kb on - strand at 4014.296 kb on - strand at 4014.353 kb on + strand at 4014.400 kb on + strand at 4014.420 kb on + strand at 4014.433 kb on - strand at 4014.438 kb on - strand at 4014.447 kb on + strand at 4014.448 kb on + strand at 4014.448 kb on + strand at 4014.456 kb on + strand at 4014.506 kb on + strand at 4014.506 kb on + strand at 4014.532 kb on - strand, within udp at 4014.605 kb on + strand, within udp at 4014.619 kb on + strand, within udp at 4014.667 kb on + strand, within udp at 4014.675 kb on + strand, within udp at 4014.683 kb on - strand, within udp at 4014.700 kb on - strand, within udp at 4014.721 kb on + strand, within udp at 4014.792 kb on + strand, within udp at 4014.795 kb on - strand, within udp at 4014.811 kb on + strand, within udp at 4014.819 kb on - strand, within udp at 4014.819 kb on - strand, within udp at 4014.871 kb on - strand, within udp at 4014.871 kb on - strand, within udp at 4014.881 kb on - strand, within udp at 4014.904 kb on + strand, within udp at 4014.936 kb on + strand, within udp at 4014.936 kb on + strand, within udp at 4014.936 kb on + strand, within udp at 4014.936 kb on + strand, within udp at 4014.943 kb on - strand, within udp at 4014.944 kb on - strand, within udp at 4014.944 kb on - strand, within udp at 4014.944 kb on - strand, within udp at 4014.944 kb on - strand, within udp at 4014.946 kb on + strand, within udp at 4014.971 kb on - strand, within udp at 4014.971 kb on - strand, within udp at 4014.975 kb on - strand, within udp at 4014.998 kb on - strand, within udp at 4014.998 kb on - strand, within udp at 4015.001 kb on + strand, within udp at 4015.001 kb on + strand, within udp at 4015.047 kb on - strand, within udp at 4015.049 kb on + strand, within udp at 4015.070 kb on + strand, within udp at 4015.076 kb on + strand, within udp at 4015.080 kb on + strand, within udp at 4015.088 kb on - strand, within udp at 4015.088 kb on - strand, within udp at 4015.092 kb on + strand, within udp at 4015.102 kb on - strand, within udp at 4015.102 kb on - strand, within udp at 4015.119 kb on - strand, within udp
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 4,012,402 - metE b3829 0.59 -0.6 4,012,466 + metE b3829 0.61 -0.4 4,012,467 + metE b3829 0.61 +1.7 4,012,474 - metE b3829 0.62 +1.6 4,012,511 + metE b3829 0.63 +1.4 4,012,511 + metE b3829 0.63 -2.6 4,012,595 - metE b3829 0.67 +0.4 4,012,600 + metE b3829 0.67 -2.2 4,012,600 + metE b3829 0.67 -1.4 4,012,606 - metE b3829 0.68 -0.9 4,012,613 - metE b3829 0.68 -1.9 4,012,613 - metE b3829 0.68 -1.6 4,012,615 - metE b3829 0.68 -1.3 4,012,663 + metE b3829 0.70 -0.6 4,012,672 + metE b3829 0.71 +1.4 4,012,672 + metE b3829 0.71 +1.7 4,012,731 + metE b3829 0.73 +1.4 4,012,731 + metE b3829 0.73 +1.7 4,012,738 - metE b3829 0.73 -3.1 4,012,738 - metE b3829 0.73 -2.9 4,012,750 - metE b3829 0.74 +2.0 4,012,820 + metE b3829 0.77 +0.4 4,012,835 + metE b3829 0.78 +0.2 4,012,861 + metE b3829 0.79 +2.4 4,012,861 + metE b3829 0.79 -2.2 4,012,866 + metE b3829 0.79 -1.4 4,012,866 + metE b3829 0.79 -1.2 4,012,935 - metE b3829 0.82 +2.5 4,012,935 - metE b3829 0.82 -0.7 4,013,004 - metE b3829 0.85 -1.5 4,013,048 + metE b3829 0.87 -2.3 4,013,048 + metE b3829 0.87 -1.4 4,013,063 - metE b3829 0.88 -0.3 4,013,135 + -0.9 4,013,135 + +1.1 4,013,183 + +0.3 4,013,192 - +1.7 4,013,192 - +0.9 4,013,193 - +0.8 4,013,193 - +0.5 4,013,224 + -1.0 4,013,235 - -0.6 4,013,235 - +1.4 4,013,250 - -1.7 4,013,250 - +0.1 4,013,250 - -2.9 4,013,250 - +2.3 4,013,359 - -0.9 4,013,359 - -2.3 4,013,359 - -0.3 4,013,393 + +1.8 4,013,401 - -3.2 4,013,403 + -0.9 4,013,406 - -1.2 4,013,417 + +0.8 4,013,502 + ysgA b3830 0.15 -2.8 4,013,531 - ysgA b3830 0.19 +0.5 4,013,540 + ysgA b3830 0.20 -0.3 4,013,540 + ysgA b3830 0.20 -1.1 4,013,560 - ysgA b3830 0.22 -4.1 4,013,678 + ysgA b3830 0.37 -1.0 4,013,723 + ysgA b3830 0.42 +0.6 4,013,723 + ysgA b3830 0.42 -2.9 4,013,735 + ysgA b3830 0.44 -1.4 4,013,735 + ysgA b3830 0.44 -2.6 4,013,736 - ysgA b3830 0.44 +1.1 4,013,737 + ysgA b3830 0.44 +2.0 4,013,742 + ysgA b3830 0.45 -1.3 4,013,742 + ysgA b3830 0.45 +1.7 4,013,747 + ysgA b3830 0.45 -0.2 4,013,781 - ysgA b3830 0.50 -2.8 4,013,822 + ysgA b3830 0.55 -2.5 4,013,858 - ysgA b3830 0.59 +1.5 4,013,860 - ysgA b3830 0.59 -2.5 4,013,872 - ysgA b3830 0.61 +0.5 4,013,952 + ysgA b3830 0.70 +0.9 4,014,006 + ysgA b3830 0.77 +0.4 4,014,030 + ysgA b3830 0.80 -0.2 4,014,045 - ysgA b3830 0.82 -1.4 4,014,056 - ysgA b3830 0.83 -1.6 4,014,066 - ysgA b3830 0.84 -2.2 4,014,066 - ysgA b3830 0.84 -1.1 4,014,084 - ysgA b3830 0.87 -0.1 4,014,084 - ysgA b3830 0.87 -1.8 4,014,086 - ysgA b3830 0.87 -1.1 4,014,124 + -1.1 4,014,124 + -1.8 4,014,183 + -2.5 4,014,194 + +1.8 4,014,198 + -0.6 4,014,260 - -1.5 4,014,260 - +0.8 4,014,296 - +1.4 4,014,296 - +1.4 4,014,353 + +0.4 4,014,400 + -2.1 4,014,420 + +2.2 4,014,433 - -3.1 4,014,438 - +1.8 4,014,447 + +0.9 4,014,448 + -2.9 4,014,448 + -0.8 4,014,456 + -0.4 4,014,506 + -0.4 4,014,506 + +0.6 4,014,532 - udp b3831 0.10 +0.1 4,014,605 + udp b3831 0.20 -2.1 4,014,619 + udp b3831 0.22 +1.6 4,014,667 + udp b3831 0.28 -1.9 4,014,675 + udp b3831 0.29 +3.5 4,014,683 - udp b3831 0.30 -0.2 4,014,700 - udp b3831 0.32 -2.9 4,014,721 + udp b3831 0.35 -3.0 4,014,792 + udp b3831 0.44 -0.9 4,014,795 - udp b3831 0.45 +0.4 4,014,811 + udp b3831 0.47 +0.7 4,014,819 - udp b3831 0.48 -1.3 4,014,819 - udp b3831 0.48 -1.7 4,014,871 - udp b3831 0.55 -0.6 4,014,871 - udp b3831 0.55 +0.0 4,014,881 - udp b3831 0.56 +0.9 4,014,904 + udp b3831 0.59 -3.0 4,014,936 + udp b3831 0.63 +0.1 4,014,936 + udp b3831 0.63 +0.7 4,014,936 + udp b3831 0.63 +0.6 4,014,936 + udp b3831 0.63 -2.9 4,014,943 - udp b3831 0.64 -2.7 4,014,944 - udp b3831 0.64 -0.6 4,014,944 - udp b3831 0.64 -1.4 4,014,944 - udp b3831 0.64 +0.3 4,014,944 - udp b3831 0.64 +0.4 4,014,946 + udp b3831 0.65 +1.2 4,014,971 - udp b3831 0.68 +0.8 4,014,971 - udp b3831 0.68 -3.0 4,014,975 - udp b3831 0.68 -0.4 4,014,998 - udp b3831 0.71 +0.1 4,014,998 - udp b3831 0.71 +0.2 4,015,001 + udp b3831 0.72 -0.6 4,015,001 + udp b3831 0.72 -1.8 4,015,047 - udp b3831 0.78 -3.6 4,015,049 + udp b3831 0.78 -0.7 4,015,070 + udp b3831 0.81 +1.2 4,015,076 + udp b3831 0.82 -0.5 4,015,080 + udp b3831 0.82 +0.6 4,015,088 - udp b3831 0.83 -2.7 4,015,088 - udp b3831 0.83 -0.4 4,015,092 + udp b3831 0.84 -3.2 4,015,102 - udp b3831 0.85 +3.7 4,015,102 - udp b3831 0.85 +2.1 4,015,119 - udp b3831 0.87 -3.2
Or see this region's nucleotide sequence