Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nikE and nikR are separated by 5 nucleotides nikR and rhsB are separated by 202 nucleotides rhsB and yhhH overlap by 29 nucleotides yhhH and yrhC are separated by 104 nucleotides yrhC and yhhI are separated by 140 nucleotides
b3480: nikE - nickel transporter subunit (NCBI), at 3,615,799 to 3,616,605
nikE
b3481: nikR - nickel responsive regulator (NCBI), at 3,616,611 to 3,617,012
nikR
b3482: rhsB - rhsB element core protein RshB (NCBI), at 3,617,215 to 3,621,450
rhsB
b3483: yhhH - orf, hypothetical protein (VIMSS), at 3,621,422 to 3,621,805
yhhH
b4552: yrhC - no description, at 3,621,910 to 3,622,260
yrhC
b3484: yhhI - predicted transposase (NCBI), at 3,622,401 to 3,623,537
yhhI
Position (kb)
3617
3618
3619
3620
3621
3622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3616.224 kb on + strand, within nikE at 3616.287 kb on - strand, within nikE at 3616.424 kb on - strand, within nikE at 3616.444 kb on - strand, within nikE at 3616.444 kb on - strand, within nikE at 3616.490 kb on + strand, within nikE at 3616.572 kb on - strand at 3616.691 kb on - strand, within nikR at 3616.721 kb on - strand, within nikR at 3616.723 kb on + strand, within nikR at 3616.743 kb on + strand, within nikR at 3616.764 kb on - strand, within nikR at 3616.793 kb on - strand, within nikR at 3616.793 kb on - strand, within nikR at 3616.815 kb on - strand, within nikR at 3616.853 kb on + strand, within nikR at 3616.853 kb on + strand, within nikR at 3616.872 kb on + strand, within nikR at 3616.932 kb on + strand, within nikR at 3616.937 kb on + strand, within nikR at 3617.048 kb on - strand at 3617.063 kb on + strand at 3617.063 kb on + strand at 3617.065 kb on - strand at 3617.069 kb on - strand at 3617.069 kb on - strand at 3617.069 kb on - strand at 3617.083 kb on - strand at 3617.083 kb on - strand at 3617.085 kb on + strand at 3617.177 kb on + strand at 3617.200 kb on + strand at 3617.251 kb on + strand at 3617.251 kb on + strand at 3617.290 kb on + strand at 3618.418 kb on - strand, within rhsB at 3620.296 kb on + strand, within rhsB at 3620.452 kb on - strand, within rhsB at 3620.452 kb on - strand, within rhsB at 3620.454 kb on - strand, within rhsB at 3620.706 kb on + strand, within rhsB at 3620.851 kb on + strand, within rhsB at 3620.851 kb on + strand, within rhsB at 3620.874 kb on + strand, within rhsB at 3620.892 kb on + strand, within rhsB at 3620.892 kb on + strand, within rhsB at 3620.896 kb on + strand, within rhsB at 3620.905 kb on - strand, within rhsB at 3620.906 kb on + strand, within rhsB at 3620.914 kb on + strand, within rhsB at 3620.939 kb on - strand, within rhsB at 3620.944 kb on + strand, within rhsB at 3620.944 kb on + strand, within rhsB at 3620.949 kb on + strand, within rhsB at 3620.949 kb on + strand, within rhsB at 3620.967 kb on - strand, within rhsB at 3620.967 kb on - strand, within rhsB at 3620.982 kb on + strand, within rhsB at 3620.985 kb on + strand, within rhsB at 3620.985 kb on + strand, within rhsB at 3620.995 kb on - strand, within rhsB at 3620.995 kb on - strand, within rhsB at 3621.002 kb on + strand, within rhsB at 3621.002 kb on + strand, within rhsB at 3621.002 kb on + strand, within rhsB at 3621.002 kb on + strand, within rhsB at 3621.010 kb on - strand, within rhsB at 3621.011 kb on - strand, within rhsB at 3621.011 kb on - strand, within rhsB at 3621.023 kb on - strand, within rhsB at 3621.046 kb on + strand at 3621.051 kb on - strand at 3621.051 kb on - strand at 3621.054 kb on - strand at 3621.054 kb on - strand at 3621.056 kb on + strand at 3621.058 kb on + strand at 3621.058 kb on + strand at 3621.058 kb on - strand at 3621.090 kb on + strand at 3621.103 kb on + strand at 3621.145 kb on + strand at 3621.150 kb on + strand at 3621.150 kb on + strand at 3621.188 kb on + strand at 3621.195 kb on + strand at 3621.198 kb on + strand at 3621.198 kb on + strand at 3621.204 kb on + strand at 3621.204 kb on + strand at 3621.209 kb on + strand at 3621.209 kb on + strand at 3621.209 kb on + strand at 3621.209 kb on + strand at 3621.209 kb on + strand at 3621.209 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.210 kb on + strand at 3621.216 kb on + strand at 3621.218 kb on + strand at 3621.220 kb on + strand at 3621.239 kb on + strand at 3621.239 kb on + strand at 3621.239 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.251 kb on + strand at 3621.255 kb on + strand at 3621.260 kb on + strand at 3621.654 kb on - strand, within yhhH at 3621.654 kb on - strand, within yhhH at 3621.816 kb on - strand at 3621.827 kb on - strand at 3621.836 kb on - strand at 3621.848 kb on - strand at 3621.848 kb on - strand at 3621.851 kb on + strand at 3621.852 kb on - strand at 3621.853 kb on - strand at 3621.858 kb on + strand at 3621.858 kb on + strand at 3621.858 kb on + strand at 3621.866 kb on + strand at 3622.073 kb on - strand, within yrhC at 3622.075 kb on - strand, within yrhC at 3622.075 kb on - strand, within yrhC at 3622.262 kb on - strand at 3622.360 kb on + strand at 3622.375 kb on + strand at 3622.375 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 3,616,224 + nikE b3480 0.53 +0.9 3,616,287 - nikE b3480 0.60 +0.0 3,616,424 - nikE b3480 0.77 -0.8 3,616,444 - nikE b3480 0.80 -2.1 3,616,444 - nikE b3480 0.80 +0.2 3,616,490 + nikE b3480 0.86 +0.7 3,616,572 - -2.8 3,616,691 - nikR b3481 0.20 -0.7 3,616,721 - nikR b3481 0.27 -0.8 3,616,723 + nikR b3481 0.28 +0.4 3,616,743 + nikR b3481 0.33 +0.5 3,616,764 - nikR b3481 0.38 +1.8 3,616,793 - nikR b3481 0.45 -1.7 3,616,793 - nikR b3481 0.45 -1.8 3,616,815 - nikR b3481 0.51 -1.0 3,616,853 + nikR b3481 0.60 +2.1 3,616,853 + nikR b3481 0.60 +2.3 3,616,872 + nikR b3481 0.65 -0.4 3,616,932 + nikR b3481 0.80 +2.7 3,616,937 + nikR b3481 0.81 -1.3 3,617,048 - -3.4 3,617,063 + +0.4 3,617,063 + -0.9 3,617,065 - -0.5 3,617,069 - +3.1 3,617,069 - -0.7 3,617,069 - -1.0 3,617,083 - -1.3 3,617,083 - +1.0 3,617,085 + -2.4 3,617,177 + +0.2 3,617,200 + +2.5 3,617,251 + +2.4 3,617,251 + +0.9 3,617,290 + +0.9 3,618,418 - rhsB b3482 0.28 -2.0 3,620,296 + rhsB b3482 0.73 +1.1 3,620,452 - rhsB b3482 0.76 -0.3 3,620,452 - rhsB b3482 0.76 +1.9 3,620,454 - rhsB b3482 0.76 +1.6 3,620,706 + rhsB b3482 0.82 -1.5 3,620,851 + rhsB b3482 0.86 +3.1 3,620,851 + rhsB b3482 0.86 -0.9 3,620,874 + rhsB b3482 0.86 -2.4 3,620,892 + rhsB b3482 0.87 +0.2 3,620,892 + rhsB b3482 0.87 +4.3 3,620,896 + rhsB b3482 0.87 +2.8 3,620,905 - rhsB b3482 0.87 +1.6 3,620,906 + rhsB b3482 0.87 +0.7 3,620,914 + rhsB b3482 0.87 -0.6 3,620,939 - rhsB b3482 0.88 -0.7 3,620,944 + rhsB b3482 0.88 -0.5 3,620,944 + rhsB b3482 0.88 -1.0 3,620,949 + rhsB b3482 0.88 +0.4 3,620,949 + rhsB b3482 0.88 -4.1 3,620,967 - rhsB b3482 0.89 -1.0 3,620,967 - rhsB b3482 0.89 +2.8 3,620,982 + rhsB b3482 0.89 +0.9 3,620,985 + rhsB b3482 0.89 +0.2 3,620,985 + rhsB b3482 0.89 -2.7 3,620,995 - rhsB b3482 0.89 -0.3 3,620,995 - rhsB b3482 0.89 -3.7 3,621,002 + rhsB b3482 0.89 -1.5 3,621,002 + rhsB b3482 0.89 -2.0 3,621,002 + rhsB b3482 0.89 -2.7 3,621,002 + rhsB b3482 0.89 +1.6 3,621,010 - rhsB b3482 0.90 +1.6 3,621,011 - rhsB b3482 0.90 -0.7 3,621,011 - rhsB b3482 0.90 +3.3 3,621,023 - rhsB b3482 0.90 +3.2 3,621,046 + +0.8 3,621,051 - +0.3 3,621,051 - -2.0 3,621,054 - +5.1 3,621,054 - -0.7 3,621,056 + -0.7 3,621,058 + +1.3 3,621,058 + -1.8 3,621,058 - -0.3 3,621,090 + -1.3 3,621,103 + +1.7 3,621,145 + +1.9 3,621,150 + -0.3 3,621,150 + -1.0 3,621,188 + +1.3 3,621,195 + +0.3 3,621,198 + +0.3 3,621,198 + -0.7 3,621,204 + +0.3 3,621,204 + +1.3 3,621,209 + +1.3 3,621,209 + -1.8 3,621,209 + +0.3 3,621,209 + +0.3 3,621,209 + -0.3 3,621,209 + -1.3 3,621,210 + -1.3 3,621,210 + +1.3 3,621,210 + +2.2 3,621,210 + +0.3 3,621,210 + +1.3 3,621,210 + -0.7 3,621,210 + +0.3 3,621,216 + +1.3 3,621,218 + +0.3 3,621,220 + -1.0 3,621,239 + -0.7 3,621,239 + +1.3 3,621,239 + -0.3 3,621,251 + -1.0 3,621,251 + -0.7 3,621,251 + +0.3 3,621,255 + -1.5 3,621,260 + -1.0 3,621,654 - yhhH b3483 0.60 +0.3 3,621,654 - yhhH b3483 0.60 +1.3 3,621,816 - +1.3 3,621,827 - +0.3 3,621,836 - +1.3 3,621,848 - +1.3 3,621,848 - -0.3 3,621,851 + +1.3 3,621,852 - +1.3 3,621,853 - +1.3 3,621,858 + +1.3 3,621,858 + +1.3 3,621,858 + +1.3 3,621,866 + +1.3 3,622,073 - yrhC b4552 0.46 +1.3 3,622,075 - yrhC b4552 0.47 +4.5 3,622,075 - yrhC b4552 0.47 -1.0 3,622,262 - -1.5 3,622,360 + +0.3 3,622,375 + +1.3 3,622,375 + +1.3
Or see this region's nucleotide sequence