Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aidB and yjfN are separated by 116 nucleotides yjfN and yjfO are separated by 148 nucleotides yjfO and yjfP are separated by 181 nucleotides yjfP and ulaR overlap by 4 nucleotides
b4187: aidB - putative acyl coenzyme A dehydrogenase (VIMSS), at 4,412,298 to 4,413,923
aidB
b4188: yjfN - orf, hypothetical protein (VIMSS), at 4,414,040 to 4,414,315
yjfN
b4189: yjfO - orf, hypothetical protein (VIMSS), at 4,414,464 to 4,414,793
yjfO
b4190: yjfP - predicted hydrolase (NCBI), at 4,414,975 to 4,415,724
yjfP
b4191: ulaR - DNA-binding transcriptional dual regulator (NCBI), at 4,415,721 to 4,416,476
ulaR
Position (kb)
4414
4415 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4413.489 kb on + strand, within aidB at 4413.521 kb on + strand, within aidB at 4413.586 kb on + strand, within aidB at 4413.690 kb on - strand, within aidB at 4413.735 kb on + strand, within aidB at 4413.735 kb on + strand, within aidB at 4413.865 kb on + strand at 4413.918 kb on + strand at 4413.918 kb on - strand at 4414.039 kb on + strand at 4414.039 kb on + strand at 4414.049 kb on - strand at 4414.049 kb on - strand at 4414.051 kb on + strand at 4414.136 kb on + strand, within yjfN at 4414.136 kb on + strand, within yjfN at 4414.142 kb on - strand, within yjfN at 4414.142 kb on - strand, within yjfN at 4414.160 kb on + strand, within yjfN at 4414.181 kb on - strand, within yjfN at 4414.206 kb on - strand, within yjfN at 4414.226 kb on - strand, within yjfN at 4414.226 kb on - strand, within yjfN at 4414.230 kb on + strand, within yjfN at 4414.252 kb on + strand, within yjfN at 4414.254 kb on + strand, within yjfN at 4414.257 kb on - strand, within yjfN at 4414.257 kb on - strand, within yjfN at 4414.345 kb on + strand at 4414.355 kb on + strand at 4414.356 kb on + strand at 4414.368 kb on + strand at 4414.368 kb on + strand at 4414.374 kb on - strand at 4414.374 kb on - strand at 4414.377 kb on - strand at 4414.377 kb on - strand at 4414.392 kb on + strand at 4414.394 kb on + strand at 4414.394 kb on + strand at 4414.409 kb on + strand at 4414.409 kb on + strand at 4414.409 kb on + strand at 4414.410 kb on - strand at 4414.415 kb on - strand at 4414.422 kb on - strand at 4414.424 kb on + strand at 4414.424 kb on + strand at 4414.431 kb on + strand at 4414.431 kb on + strand at 4414.434 kb on + strand at 4414.434 kb on + strand at 4414.442 kb on - strand at 4414.448 kb on + strand at 4414.448 kb on + strand at 4414.456 kb on - strand at 4414.499 kb on + strand, within yjfO at 4414.523 kb on - strand, within yjfO at 4414.599 kb on + strand, within yjfO at 4414.599 kb on + strand, within yjfO at 4414.613 kb on + strand, within yjfO at 4414.617 kb on - strand, within yjfO at 4414.621 kb on - strand, within yjfO at 4414.621 kb on - strand, within yjfO at 4414.621 kb on - strand, within yjfO at 4414.651 kb on - strand, within yjfO at 4414.682 kb on - strand, within yjfO at 4414.682 kb on - strand, within yjfO at 4414.704 kb on + strand, within yjfO at 4414.705 kb on - strand, within yjfO at 4414.716 kb on + strand, within yjfO at 4414.722 kb on - strand, within yjfO at 4414.746 kb on - strand, within yjfO at 4414.791 kb on - strand at 4414.791 kb on - strand at 4414.795 kb on + strand at 4414.800 kb on - strand at 4414.800 kb on - strand at 4414.825 kb on - strand at 4414.838 kb on - strand at 4414.838 kb on - strand at 4414.885 kb on + strand at 4414.899 kb on + strand at 4414.899 kb on + strand at 4415.030 kb on - strand at 4415.030 kb on - strand at 4415.050 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.086 kb on - strand, within yjfP at 4415.103 kb on - strand, within yjfP at 4415.171 kb on + strand, within yjfP at 4415.171 kb on + strand, within yjfP at 4415.370 kb on + strand, within yjfP at 4415.370 kb on + strand, within yjfP at 4415.399 kb on + strand, within yjfP at 4415.399 kb on + strand, within yjfP at 4415.526 kb on - strand, within yjfP at 4415.562 kb on - strand, within yjfP at 4415.590 kb on - strand, within yjfP at 4415.620 kb on + strand, within yjfP at 4415.620 kb on + strand, within yjfP at 4415.632 kb on + strand, within yjfP at 4415.632 kb on + strand, within yjfP at 4415.657 kb on - strand at 4415.724 kb on - strand at 4415.724 kb on - strand at 4415.767 kb on - strand at 4415.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 4,413,489 + aidB b4187 0.73 -2.6 4,413,521 + aidB b4187 0.75 +1.2 4,413,586 + aidB b4187 0.79 -1.1 4,413,690 - aidB b4187 0.86 +1.6 4,413,735 + aidB b4187 0.88 -2.7 4,413,735 + aidB b4187 0.88 +0.6 4,413,865 + -0.6 4,413,918 + +0.3 4,413,918 - +0.5 4,414,039 + -2.0 4,414,039 + +1.3 4,414,049 - +0.2 4,414,049 - -0.1 4,414,051 + -0.1 4,414,136 + yjfN b4188 0.35 +1.0 4,414,136 + yjfN b4188 0.35 +0.0 4,414,142 - yjfN b4188 0.37 -0.9 4,414,142 - yjfN b4188 0.37 -2.4 4,414,160 + yjfN b4188 0.43 +3.0 4,414,181 - yjfN b4188 0.51 -1.9 4,414,206 - yjfN b4188 0.60 -1.1 4,414,226 - yjfN b4188 0.67 -1.1 4,414,226 - yjfN b4188 0.67 -0.1 4,414,230 + yjfN b4188 0.69 -1.7 4,414,252 + yjfN b4188 0.77 +0.4 4,414,254 + yjfN b4188 0.78 +0.7 4,414,257 - yjfN b4188 0.79 +2.2 4,414,257 - yjfN b4188 0.79 +1.0 4,414,345 + +1.9 4,414,355 + -0.1 4,414,356 + -0.0 4,414,368 + -1.0 4,414,368 + -0.7 4,414,374 - -2.5 4,414,374 - +0.0 4,414,377 - +1.2 4,414,377 - -2.1 4,414,392 + +2.0 4,414,394 + +2.2 4,414,394 + +3.5 4,414,409 + -3.1 4,414,409 + -0.6 4,414,409 + -1.5 4,414,410 - +0.4 4,414,415 - +1.4 4,414,422 - +1.5 4,414,424 + +2.3 4,414,424 + +0.7 4,414,431 + +0.6 4,414,431 + +1.9 4,414,434 + +2.6 4,414,434 + +0.7 4,414,442 - +1.4 4,414,448 + +0.7 4,414,448 + +0.0 4,414,456 - -4.0 4,414,499 + yjfO b4189 0.11 +0.9 4,414,523 - yjfO b4189 0.18 -0.9 4,414,599 + yjfO b4189 0.41 -0.6 4,414,599 + yjfO b4189 0.41 -1.9 4,414,613 + yjfO b4189 0.45 +1.0 4,414,617 - yjfO b4189 0.46 +3.0 4,414,621 - yjfO b4189 0.48 -1.6 4,414,621 - yjfO b4189 0.48 +1.7 4,414,621 - yjfO b4189 0.48 +1.4 4,414,651 - yjfO b4189 0.57 +1.1 4,414,682 - yjfO b4189 0.66 -0.9 4,414,682 - yjfO b4189 0.66 +1.8 4,414,704 + yjfO b4189 0.73 -1.0 4,414,705 - yjfO b4189 0.73 +0.1 4,414,716 + yjfO b4189 0.76 +3.7 4,414,722 - yjfO b4189 0.78 -0.3 4,414,746 - yjfO b4189 0.85 +0.3 4,414,791 - +0.5 4,414,791 - +1.6 4,414,795 + +0.6 4,414,800 - +0.7 4,414,800 - -0.6 4,414,825 - -3.1 4,414,838 - -1.7 4,414,838 - +1.6 4,414,885 + -3.7 4,414,899 + +1.2 4,414,899 + +1.2 4,415,030 - +2.1 4,415,030 - -0.1 4,415,050 - yjfP b4190 0.10 +1.9 4,415,058 - yjfP b4190 0.11 +1.8 4,415,058 - yjfP b4190 0.11 +1.4 4,415,058 - yjfP b4190 0.11 +2.0 4,415,086 - yjfP b4190 0.15 -1.1 4,415,103 - yjfP b4190 0.17 +0.9 4,415,171 + yjfP b4190 0.26 +3.3 4,415,171 + yjfP b4190 0.26 +2.1 4,415,370 + yjfP b4190 0.53 -1.1 4,415,370 + yjfP b4190 0.53 -2.1 4,415,399 + yjfP b4190 0.57 +1.5 4,415,399 + yjfP b4190 0.57 -0.1 4,415,526 - yjfP b4190 0.73 -0.1 4,415,562 - yjfP b4190 0.78 +1.9 4,415,590 - yjfP b4190 0.82 +1.1 4,415,620 + yjfP b4190 0.86 -0.9 4,415,620 + yjfP b4190 0.86 -0.1 4,415,632 + yjfP b4190 0.88 -1.9 4,415,632 + yjfP b4190 0.88 +3.0 4,415,657 - -1.3 4,415,724 - +3.7 4,415,724 - +0.4 4,415,767 - +1.1 4,415,767 - +0.4
Or see this region's nucleotide sequence