Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfE and nrfF overlap by 8 nucleotides nrfF and nrfG overlap by 4 nucleotides nrfG and gltP are separated by 341 nucleotides
b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193
nrfE
b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569
nrfF
b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162
nrfG
b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817
gltP
Position (kb)
4291
4292 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4290.255 kb on + strand, within nrfE at 4290.255 kb on + strand, within nrfE at 4290.265 kb on - strand, within nrfE at 4290.415 kb on - strand, within nrfE at 4290.415 kb on - strand, within nrfE at 4290.441 kb on - strand, within nrfE at 4290.443 kb on + strand, within nrfE at 4290.443 kb on + strand, within nrfE at 4290.465 kb on + strand, within nrfE at 4290.465 kb on - strand, within nrfE at 4290.465 kb on - strand, within nrfE at 4290.566 kb on + strand, within nrfE at 4290.572 kb on - strand, within nrfE at 4290.619 kb on + strand, within nrfE at 4290.619 kb on + strand, within nrfE at 4290.726 kb on + strand, within nrfE at 4290.797 kb on - strand, within nrfE at 4290.832 kb on + strand, within nrfE at 4290.839 kb on + strand, within nrfE at 4290.839 kb on + strand, within nrfE at 4290.847 kb on - strand, within nrfE at 4290.930 kb on + strand, within nrfE at 4290.952 kb on + strand, within nrfE at 4291.075 kb on + strand at 4291.075 kb on + strand at 4291.155 kb on + strand at 4291.208 kb on - strand at 4291.236 kb on + strand, within nrfF at 4291.270 kb on + strand, within nrfF at 4291.423 kb on - strand, within nrfF at 4291.464 kb on + strand, within nrfF at 4291.472 kb on - strand, within nrfF at 4291.483 kb on + strand, within nrfF at 4291.497 kb on + strand, within nrfF at 4291.503 kb on + strand, within nrfF at 4291.503 kb on + strand, within nrfF at 4291.711 kb on - strand, within nrfG at 4291.800 kb on - strand, within nrfG at 4291.854 kb on + strand, within nrfG at 4291.859 kb on + strand, within nrfG at 4291.911 kb on + strand, within nrfG at 4291.911 kb on + strand, within nrfG at 4291.934 kb on + strand, within nrfG at 4291.982 kb on + strand, within nrfG at 4291.982 kb on + strand, within nrfG at 4292.106 kb on + strand at 4292.109 kb on + strand at 4292.112 kb on - strand at 4292.112 kb on - strand at 4292.114 kb on + strand at 4292.114 kb on + strand at 4292.131 kb on - strand at 4292.368 kb on + strand at 4292.368 kb on + strand at 4292.388 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 4,290,255 + nrfE b4074 0.43 +0.2 4,290,255 + nrfE b4074 0.43 +2.1 4,290,265 - nrfE b4074 0.44 +0.6 4,290,415 - nrfE b4074 0.53 -0.5 4,290,415 - nrfE b4074 0.53 +1.6 4,290,441 - nrfE b4074 0.55 -2.5 4,290,443 + nrfE b4074 0.55 +2.0 4,290,443 + nrfE b4074 0.55 -2.5 4,290,465 + nrfE b4074 0.56 -0.8 4,290,465 - nrfE b4074 0.56 -0.9 4,290,465 - nrfE b4074 0.56 -0.9 4,290,566 + nrfE b4074 0.62 -3.4 4,290,572 - nrfE b4074 0.63 -1.1 4,290,619 + nrfE b4074 0.65 -3.2 4,290,619 + nrfE b4074 0.65 -1.2 4,290,726 + nrfE b4074 0.72 -3.6 4,290,797 - nrfE b4074 0.76 +1.1 4,290,832 + nrfE b4074 0.78 +0.1 4,290,839 + nrfE b4074 0.79 +1.5 4,290,839 + nrfE b4074 0.79 -0.1 4,290,847 - nrfE b4074 0.79 +2.5 4,290,930 + nrfE b4074 0.84 -2.0 4,290,952 + nrfE b4074 0.85 -1.4 4,291,075 + -2.5 4,291,075 + -0.8 4,291,155 + -0.8 4,291,208 - -2.5 4,291,236 + nrfF b4075 0.13 +1.5 4,291,270 + nrfF b4075 0.22 +0.1 4,291,423 - nrfF b4075 0.62 +2.0 4,291,464 + nrfF b4075 0.72 +2.2 4,291,472 - nrfF b4075 0.74 +1.1 4,291,483 + nrfF b4075 0.77 +1.0 4,291,497 + nrfF b4075 0.81 +1.3 4,291,503 + nrfF b4075 0.83 -1.3 4,291,503 + nrfF b4075 0.83 -3.5 4,291,711 - nrfG b4076 0.24 -0.3 4,291,800 - nrfG b4076 0.39 -0.2 4,291,854 + nrfG b4076 0.48 -2.5 4,291,859 + nrfG b4076 0.49 -0.1 4,291,911 + nrfG b4076 0.58 -0.7 4,291,911 + nrfG b4076 0.58 -0.9 4,291,934 + nrfG b4076 0.62 -1.7 4,291,982 + nrfG b4076 0.70 -1.5 4,291,982 + nrfG b4076 0.70 +1.6 4,292,106 + -1.1 4,292,109 + -0.0 4,292,112 - -2.1 4,292,112 - -3.1 4,292,114 + +0.7 4,292,114 + -1.5 4,292,131 - +2.3 4,292,368 + +1.0 4,292,368 + +0.9 4,292,388 + +0.4 4,292,479 + -0.2 4,292,479 + +0.8 4,292,479 + -0.5 4,292,479 + +0.0 4,292,479 + -0.5
Or see this region's nucleotide sequence