Strain Fitness in Escherichia coli BW25113 around b2712

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnorW and hypF are separated by 127 nucleotideshypF and hydN are separated by 152 nucleotideshydN and ascG are separated by 148 nucleotides b2711: norW - nitric oxide reductase (NCBI), at 2,831,934 to 2,833,067 norW b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447 hypF b2713: hydN - formate dehydrogenase-H, [4Fe-4S] ferredoxin subunit (NCBI), at 2,835,600 to 2,836,127 hydN b2714: ascG - DNA-binding transcriptional repressor (NCBI), at 2,836,276 to 2,837,286 ascG Position (kb) 2833 2834 2835 2836Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2832.281 kb on + strand, within norWat 2832.281 kb on + strand, within norWat 2832.364 kb on + strand, within norWat 2832.384 kb on - strand, within norWat 2832.384 kb on - strand, within norWat 2832.526 kb on + strand, within norWat 2832.896 kb on - strand, within norWat 2832.956 kb on + strandat 2833.005 kb on + strandat 2833.005 kb on + strandat 2833.046 kb on + strandat 2833.054 kb on - strandat 2833.119 kb on + strandat 2833.119 kb on + strandat 2833.350 kb on + strandat 2833.350 kb on + strandat 2833.353 kb on - strandat 2833.371 kb on + strandat 2833.399 kb on - strandat 2833.399 kb on - strandat 2833.408 kb on + strandat 2833.423 kb on + strand, within hypFat 2833.423 kb on + strand, within hypFat 2833.581 kb on + strand, within hypFat 2833.699 kb on + strand, within hypFat 2833.734 kb on + strand, within hypFat 2833.934 kb on - strand, within hypFat 2833.962 kb on + strand, within hypFat 2833.962 kb on + strand, within hypFat 2833.980 kb on - strand, within hypFat 2834.076 kb on - strand, within hypFat 2834.208 kb on - strand, within hypFat 2834.211 kb on + strand, within hypFat 2834.211 kb on + strand, within hypFat 2834.358 kb on + strand, within hypFat 2834.547 kb on + strand, within hypFat 2834.574 kb on + strand, within hypFat 2834.574 kb on + strand, within hypFat 2834.576 kb on - strand, within hypFat 2834.576 kb on - strand, within hypFat 2834.689 kb on - strand, within hypFat 2834.832 kb on - strand, within hypFat 2834.862 kb on + strand, within hypFat 2834.878 kb on - strand, within hypFat 2834.951 kb on - strand, within hypFat 2834.951 kb on - strand, within hypFat 2834.955 kb on + strand, within hypFat 2834.972 kb on + strand, within hypFat 2835.007 kb on + strand, within hypFat 2835.007 kb on + strand, within hypFat 2835.051 kb on + strand, within hypFat 2835.051 kb on + strand, within hypFat 2835.172 kb on - strand, within hypFat 2835.172 kb on - strand, within hypFat 2835.235 kb on - strandat 2835.264 kb on - strandat 2835.306 kb on + strandat 2835.415 kb on + strandat 2835.522 kb on - strandat 2835.522 kb on - strandat 2835.729 kb on - strand, within hydNat 2835.731 kb on - strand, within hydNat 2835.812 kb on - strand, within hydNat 2835.849 kb on - strand, within hydNat 2835.849 kb on - strand, within hydNat 2835.902 kb on + strand, within hydNat 2835.933 kb on + strand, within hydNat 2835.982 kb on + strand, within hydNat 2836.033 kb on - strand, within hydNat 2836.033 kb on - strand, within hydNat 2836.043 kb on - strand, within hydNat 2836.043 kb on - strand, within hydNat 2836.078 kb on - strandat 2836.436 kb on + strand, within ascGat 2836.444 kb on - strand, within ascGat 2836.444 kb on - strand, within ascGat 2836.444 kb on - strand, within ascG

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,832,281 + norW b2711 0.31 -0.5
2,832,281 + norW b2711 0.31 +0.2
2,832,364 + norW b2711 0.38 -0.5
2,832,384 - norW b2711 0.40 -0.9
2,832,384 - norW b2711 0.40 -1.7
2,832,526 + norW b2711 0.52 -0.4
2,832,896 - norW b2711 0.85 +2.2
2,832,956 + +2.5
2,833,005 + +1.6
2,833,005 + -1.0
2,833,046 + +0.8
2,833,054 - +0.0
2,833,119 + -0.2
2,833,119 + +0.5
2,833,350 + -2.1
2,833,350 + +0.7
2,833,353 - -1.5
2,833,371 + +2.7
2,833,399 - -2.4
2,833,399 - -2.5
2,833,408 + -2.1
2,833,423 + hypF b2712 0.10 -4.1
2,833,423 + hypF b2712 0.10 +2.5
2,833,581 + hypF b2712 0.17 -2.2
2,833,699 + hypF b2712 0.22 +2.2
2,833,734 + hypF b2712 0.24 +1.5
2,833,934 - hypF b2712 0.33 -2.5
2,833,962 + hypF b2712 0.34 +0.6
2,833,962 + hypF b2712 0.34 +1.3
2,833,980 - hypF b2712 0.35 +1.0
2,834,076 - hypF b2712 0.39 +1.0
2,834,208 - hypF b2712 0.45 +1.9
2,834,211 + hypF b2712 0.45 +1.6
2,834,211 + hypF b2712 0.45 +2.8
2,834,358 + hypF b2712 0.52 -1.4
2,834,547 + hypF b2712 0.60 -3.1
2,834,574 + hypF b2712 0.61 -0.5
2,834,574 + hypF b2712 0.61 -1.7
2,834,576 - hypF b2712 0.61 -0.5
2,834,576 - hypF b2712 0.61 +1.3
2,834,689 - hypF b2712 0.66 +0.5
2,834,832 - hypF b2712 0.73 +0.2
2,834,862 + hypF b2712 0.74 -0.4
2,834,878 - hypF b2712 0.75 +1.5
2,834,951 - hypF b2712 0.78 -0.5
2,834,951 - hypF b2712 0.78 +1.3
2,834,955 + hypF b2712 0.78 +1.5
2,834,972 + hypF b2712 0.79 -2.6
2,835,007 + hypF b2712 0.80 -2.4
2,835,007 + hypF b2712 0.80 -2.4
2,835,051 + hypF b2712 0.82 +1.4
2,835,051 + hypF b2712 0.82 -1.7
2,835,172 - hypF b2712 0.88 +1.0
2,835,172 - hypF b2712 0.88 +2.5
2,835,235 - +0.0
2,835,264 - +1.2
2,835,306 + -3.6
2,835,415 + -1.8
2,835,522 - -1.1
2,835,522 - -3.1
2,835,729 - hydN b2713 0.24 +0.0
2,835,731 - hydN b2713 0.25 -1.2
2,835,812 - hydN b2713 0.40 +0.3
2,835,849 - hydN b2713 0.47 -2.1
2,835,849 - hydN b2713 0.47 +1.5
2,835,902 + hydN b2713 0.57 +1.9
2,835,933 + hydN b2713 0.63 -2.0
2,835,982 + hydN b2713 0.72 +1.0
2,836,033 - hydN b2713 0.82 +1.1
2,836,033 - hydN b2713 0.82 -0.5
2,836,043 - hydN b2713 0.84 +1.8
2,836,043 - hydN b2713 0.84 +0.8
2,836,078 - -3.5
2,836,436 + ascG b2714 0.16 -2.2
2,836,444 - ascG b2714 0.17 -1.9
2,836,444 - ascG b2714 0.17 -1.4
2,836,444 - ascG b2714 0.17 +0.2

Or see this region's nucleotide sequence