Strain Fitness in Escherichia coli BW25113 around b2450

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyffQ and yffR are separated by 10 nucleotidesyffR and yffS are separated by 150 nucleotidesyffS and eutA are separated by 148 nucleotides b2448: yffQ - CPZ-55 prophage; predicted protein (RefSeq), at 2,561,614 to 2,561,991 yffQ b2449: yffR - CPZ-55 prophage; predicted protein (NCBI), at 2,562,002 to 2,562,394 yffR b2450: yffS - CPZ-55 prophage; predicted protein (RefSeq), at 2,562,545 to 2,563,354 yffS b2451: eutA - reactivating factor for ethanolamine ammonia lyase (NCBI), at 2,563,503 to 2,564,906 eutA Position (kb) 2562 2563 2564Strain fitness (log2 ratio) -3 -2 -1 0 1at 2561.561 kb on + strandat 2561.561 kb on + strandat 2561.607 kb on - strandat 2561.652 kb on - strand, within yffQat 2561.668 kb on - strand, within yffQat 2561.668 kb on - strand, within yffQat 2561.805 kb on + strand, within yffQat 2561.872 kb on - strand, within yffQat 2561.872 kb on - strand, within yffQat 2561.872 kb on - strand, within yffQat 2561.964 kb on + strandat 2561.964 kb on + strandat 2562.073 kb on + strand, within yffRat 2562.073 kb on + strand, within yffRat 2562.092 kb on + strand, within yffRat 2562.092 kb on + strand, within yffRat 2562.114 kb on + strand, within yffRat 2562.122 kb on - strand, within yffRat 2562.140 kb on - strand, within yffRat 2562.169 kb on - strand, within yffRat 2562.407 kb on - strandat 2562.416 kb on + strandat 2562.607 kb on - strandat 2562.611 kb on + strandat 2562.611 kb on + strandat 2562.693 kb on - strand, within yffSat 2562.777 kb on + strand, within yffSat 2562.777 kb on + strand, within yffSat 2563.370 kb on - strandat 2563.429 kb on - strandat 2563.754 kb on - strand, within eutAat 2563.754 kb on - strand, within eutAat 2563.862 kb on - strand, within eutAat 2563.862 kb on - strand, within eutAat 2563.913 kb on - strand, within eutAat 2563.949 kb on - strand, within eutAat 2564.001 kb on + strand, within eutAat 2564.037 kb on - strand, within eutAat 2564.285 kb on + strand, within eutA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,561,561 + -0.4
2,561,561 + +0.6
2,561,607 - -1.5
2,561,652 - yffQ b2448 0.10 -2.5
2,561,668 - yffQ b2448 0.14 -1.2
2,561,668 - yffQ b2448 0.14 +1.4
2,561,805 + yffQ b2448 0.51 +0.9
2,561,872 - yffQ b2448 0.68 -2.9
2,561,872 - yffQ b2448 0.68 +1.3
2,561,872 - yffQ b2448 0.68 -1.0
2,561,964 + -1.7
2,561,964 + -1.7
2,562,073 + yffR b2449 0.18 +0.5
2,562,073 + yffR b2449 0.18 -2.3
2,562,092 + yffR b2449 0.23 -1.2
2,562,092 + yffR b2449 0.23 -3.2
2,562,114 + yffR b2449 0.28 -2.1
2,562,122 - yffR b2449 0.31 +1.1
2,562,140 - yffR b2449 0.35 +0.6
2,562,169 - yffR b2449 0.42 -1.0
2,562,407 - +0.2
2,562,416 + -3.2
2,562,607 - -1.9
2,562,611 + -0.2
2,562,611 + -0.3
2,562,693 - yffS b2450 0.18 -1.2
2,562,777 + yffS b2450 0.29 -1.2
2,562,777 + yffS b2450 0.29 -1.0
2,563,370 - +1.2
2,563,429 - +1.6
2,563,754 - eutA b2451 0.18 -0.4
2,563,754 - eutA b2451 0.18 -3.0
2,563,862 - eutA b2451 0.26 -0.3
2,563,862 - eutA b2451 0.26 +0.4
2,563,913 - eutA b2451 0.29 -1.0
2,563,949 - eutA b2451 0.32 +0.3
2,564,001 + eutA b2451 0.35 -0.3
2,564,037 - eutA b2451 0.38 +0.0
2,564,285 + eutA b2451 0.56 +0.7

Or see this region's nucleotide sequence