Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfbG and yfbH overlap by 4 nucleotides yfbH and arnT overlap by 1 nucleotides arnT and yfbW overlap by 4 nucleotides yfbW and b2258 overlap by 1 nucleotides b2258 and pmrD overlap by 7 nucleotides
b2255: yfbG - hypothetical protein (NCBI), at 2,366,061 to 2,368,043
yfbG
b2256: yfbH - hypothetical protein (NCBI), at 2,368,040 to 2,368,930
yfbH
b2257: arnT - 4-amino-4-deoxy-L-arabinose transferase (NCBI), at 2,368,930 to 2,370,582
arnT
b4544: yfbW - hypothetical protein (NCBI), at 2,370,579 to 2,370,914
yfbW
b2258: b2258 - putative transport/receptor protein (VIMSS), at 2,370,914 to 2,371,300
b2258
b2259: pmrD - polymyxin resistance protein B (VIMSS), at 2,371,294 to 2,371,560
pmrD
Position (kb)
2368
2369
2370
2371 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2367.936 kb on + strand at 2368.161 kb on + strand, within yfbH at 2368.161 kb on + strand, within yfbH at 2368.165 kb on + strand, within yfbH at 2368.165 kb on + strand, within yfbH at 2368.358 kb on + strand, within yfbH at 2368.453 kb on - strand, within yfbH at 2368.600 kb on + strand, within yfbH at 2368.608 kb on - strand, within yfbH at 2368.633 kb on + strand, within yfbH at 2368.633 kb on + strand, within yfbH at 2368.668 kb on - strand, within yfbH at 2368.691 kb on + strand, within yfbH at 2368.796 kb on + strand, within yfbH at 2368.805 kb on + strand, within yfbH at 2368.834 kb on + strand, within yfbH at 2368.857 kb on + strand at 2368.892 kb on + strand at 2368.923 kb on + strand at 2368.990 kb on - strand at 2369.236 kb on - strand, within arnT at 2369.265 kb on - strand, within arnT at 2369.327 kb on + strand, within arnT at 2369.327 kb on + strand, within arnT at 2369.668 kb on + strand, within arnT at 2369.668 kb on + strand, within arnT at 2369.742 kb on + strand, within arnT at 2369.762 kb on - strand, within arnT at 2369.762 kb on - strand, within arnT at 2369.881 kb on - strand, within arnT at 2370.286 kb on - strand, within arnT at 2370.294 kb on - strand, within arnT at 2370.308 kb on + strand, within arnT at 2370.308 kb on + strand, within arnT at 2370.326 kb on - strand, within arnT at 2370.493 kb on + strand at 2370.551 kb on + strand at 2370.608 kb on - strand at 2370.715 kb on + strand, within yfbW at 2370.722 kb on - strand, within yfbW at 2370.723 kb on - strand, within yfbW at 2370.723 kb on - strand, within yfbW at 2370.747 kb on + strand, within yfbW at 2370.747 kb on + strand, within yfbW at 2370.909 kb on + strand at 2370.909 kb on - strand at 2370.911 kb on + strand at 2371.053 kb on + strand, within b2258 at 2371.397 kb on - strand, within pmrD at 2371.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 2,367,936 + +0.1 2,368,161 + yfbH b2256 0.14 -1.8 2,368,161 + yfbH b2256 0.14 -3.9 2,368,165 + yfbH b2256 0.14 +0.5 2,368,165 + yfbH b2256 0.14 -2.8 2,368,358 + yfbH b2256 0.36 +0.8 2,368,453 - yfbH b2256 0.46 +1.4 2,368,600 + yfbH b2256 0.63 +1.0 2,368,608 - yfbH b2256 0.64 +1.2 2,368,633 + yfbH b2256 0.67 +0.3 2,368,633 + yfbH b2256 0.67 -2.1 2,368,668 - yfbH b2256 0.70 +0.6 2,368,691 + yfbH b2256 0.73 +1.7 2,368,796 + yfbH b2256 0.85 -2.1 2,368,805 + yfbH b2256 0.86 +1.0 2,368,834 + yfbH b2256 0.89 -0.0 2,368,857 + -0.3 2,368,892 + +0.1 2,368,923 + +0.8 2,368,990 - +0.6 2,369,236 - arnT b2257 0.19 +1.6 2,369,265 - arnT b2257 0.20 -1.8 2,369,327 + arnT b2257 0.24 +0.8 2,369,327 + arnT b2257 0.24 +1.1 2,369,668 + arnT b2257 0.45 +3.0 2,369,668 + arnT b2257 0.45 -0.4 2,369,742 + arnT b2257 0.49 -0.4 2,369,762 - arnT b2257 0.50 -1.9 2,369,762 - arnT b2257 0.50 -2.1 2,369,881 - arnT b2257 0.58 +1.6 2,370,286 - arnT b2257 0.82 +1.6 2,370,294 - arnT b2257 0.83 +1.3 2,370,308 + arnT b2257 0.83 +2.4 2,370,308 + arnT b2257 0.83 +3.2 2,370,326 - arnT b2257 0.84 +1.7 2,370,493 + -1.4 2,370,551 + +2.7 2,370,608 - -2.3 2,370,715 + yfbW b4544 0.40 -1.3 2,370,722 - yfbW b4544 0.43 -1.8 2,370,723 - yfbW b4544 0.43 -2.4 2,370,723 - yfbW b4544 0.43 +3.6 2,370,747 + yfbW b4544 0.50 -1.0 2,370,747 + yfbW b4544 0.50 -1.4 2,370,909 + +0.4 2,370,909 - +2.2 2,370,911 + -2.3 2,371,053 + b2258 b2258 0.36 +0.8 2,371,397 - pmrD b2259 0.39 -2.8 2,371,562 - -2.0
Or see this region's nucleotide sequence