Strain Fitness in Escherichia coli BW25113 around b1990

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnac and asnV are separated by 326 nucleotidesasnV and erfK are separated by 55 nucleotideserfK and cobT are separated by 64 nucleotides b1988: nac - DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI), at 2,059,040 to 2,059,957 nac b1989: asnV - tRNA-Asn (NCBI), at 2,060,284 to 2,060,359 asnV b1990: erfK - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,060,415 to 2,061,347 erfK b1991: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI), at 2,061,412 to 2,062,491 cobT Position (kb) 2060 2061 2062Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2059.487 kb on + strand, within nacat 2059.541 kb on - strand, within nacat 2059.584 kb on + strand, within nacat 2059.584 kb on + strand, within nacat 2059.643 kb on + strand, within nacat 2059.714 kb on - strand, within nacat 2059.896 kb on + strandat 2060.029 kb on + strandat 2060.165 kb on + strandat 2060.263 kb on - strandat 2060.346 kb on + strand, within asnVat 2060.520 kb on + strand, within erfKat 2060.520 kb on + strand, within erfKat 2060.530 kb on - strand, within erfKat 2060.536 kb on + strand, within erfKat 2060.573 kb on + strand, within erfKat 2060.610 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.715 kb on + strand, within erfKat 2060.730 kb on + strand, within erfKat 2060.730 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.821 kb on - strand, within erfKat 2060.976 kb on - strand, within erfKat 2060.976 kb on - strand, within erfKat 2060.983 kb on + strand, within erfKat 2060.989 kb on - strand, within erfKat 2061.007 kb on - strand, within erfKat 2061.078 kb on - strand, within erfKat 2061.200 kb on - strand, within erfKat 2061.211 kb on - strand, within erfKat 2061.216 kb on - strand, within erfKat 2061.365 kb on - strandat 2061.365 kb on - strandat 2061.426 kb on - strandat 2061.426 kb on - strandat 2061.517 kb on + strandat 2061.734 kb on + strand, within cobTat 2061.734 kb on + strand, within cobTat 2061.871 kb on + strand, within cobTat 2061.871 kb on + strand, within cobTat 2061.890 kb on - strand, within cobTat 2061.955 kb on - strand, within cobTat 2061.955 kb on - strand, within cobTat 2061.974 kb on + strand, within cobTat 2062.013 kb on - strand, within cobTat 2062.105 kb on + strand, within cobTat 2062.134 kb on - strand, within cobTat 2062.134 kb on - strand, within cobTat 2062.238 kb on - strand, within cobTat 2062.238 kb on - strand, within cobTat 2062.333 kb on + strand, within cobTat 2062.333 kb on + strand, within cobT

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,059,487 + nac b1988 0.49 -0.4
2,059,541 - nac b1988 0.55 +1.5
2,059,584 + nac b1988 0.59 -1.1
2,059,584 + nac b1988 0.59 +0.8
2,059,643 + nac b1988 0.66 +1.9
2,059,714 - nac b1988 0.73 +1.0
2,059,896 + +2.6
2,060,029 + +1.4
2,060,165 + -2.1
2,060,263 - +1.0
2,060,346 + asnV b1989 0.82 +1.4
2,060,520 + erfK b1990 0.11 -2.3
2,060,520 + erfK b1990 0.11 +2.2
2,060,530 - erfK b1990 0.12 +1.7
2,060,536 + erfK b1990 0.13 +2.3
2,060,573 + erfK b1990 0.17 +0.4
2,060,610 - erfK b1990 0.21 +0.9
2,060,630 - erfK b1990 0.23 +0.0
2,060,630 - erfK b1990 0.23 +0.6
2,060,715 + erfK b1990 0.32 +0.7
2,060,730 + erfK b1990 0.34 -1.8
2,060,730 - erfK b1990 0.34 -3.2
2,060,743 - erfK b1990 0.35 -2.1
2,060,743 - erfK b1990 0.35 +0.6
2,060,821 - erfK b1990 0.44 +2.6
2,060,976 - erfK b1990 0.60 -0.9
2,060,976 - erfK b1990 0.60 +0.6
2,060,983 + erfK b1990 0.61 +1.6
2,060,989 - erfK b1990 0.62 -0.2
2,061,007 - erfK b1990 0.63 -2.3
2,061,078 - erfK b1990 0.71 -1.1
2,061,200 - erfK b1990 0.84 +0.5
2,061,211 - erfK b1990 0.85 +1.9
2,061,216 - erfK b1990 0.86 -1.3
2,061,365 - +1.7
2,061,365 - +0.3
2,061,426 - -2.4
2,061,426 - +0.7
2,061,517 + -1.1
2,061,734 + cobT b1991 0.30 +3.7
2,061,734 + cobT b1991 0.30 -0.3
2,061,871 + cobT b1991 0.42 +0.8
2,061,871 + cobT b1991 0.42 -1.0
2,061,890 - cobT b1991 0.44 +1.9
2,061,955 - cobT b1991 0.50 +1.7
2,061,955 - cobT b1991 0.50 -0.9
2,061,974 + cobT b1991 0.52 +2.2
2,062,013 - cobT b1991 0.56 -2.2
2,062,105 + cobT b1991 0.64 +3.0
2,062,134 - cobT b1991 0.67 +2.0
2,062,134 - cobT b1991 0.67 -1.5
2,062,238 - cobT b1991 0.76 -0.9
2,062,238 - cobT b1991 0.76 -1.3
2,062,333 + cobT b1991 0.85 +1.3
2,062,333 + cobT b1991 0.85 +0.2

Or see this region's nucleotide sequence