Strain Fitness in Escherichia coli BW25113 around b1908

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttyrP and yecA are separated by 61 nucleotidesyecA and leuZ are separated by 195 nucleotidesleuZ and cysT are separated by 12 nucleotidescysT and glyW are separated by 54 nucleotidesglyW and pgsA are separated by 151 nucleotides b1907: tyrP - tyrosine transporter (NCBI), at 1,987,705 to 1,988,916 tyrP b1908: yecA - conserved metal-binding protein (NCBI), at 1,988,978 to 1,989,643 yecA b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925 leuZ b1910: cysT - tRNA-Cys (NCBI), at 1,989,938 to 1,990,011 cysT b1911: glyW - tRNA-Gly (NCBI), at 1,990,066 to 1,990,141 glyW b1912: pgsA - phosphatidylglycerophosphate synthetase (NCBI), at 1,990,293 to 1,990,841 pgsA Position (kb) 1988 1989 1990Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1988.066 kb on - strand, within tyrPat 1988.183 kb on + strand, within tyrPat 1988.309 kb on - strand, within tyrPat 1988.344 kb on - strand, within tyrPat 1988.420 kb on + strand, within tyrPat 1988.420 kb on + strand, within tyrPat 1988.478 kb on + strand, within tyrPat 1988.706 kb on - strand, within tyrPat 1988.860 kb on + strandat 1988.898 kb on + strandat 1988.957 kb on - strandat 1989.096 kb on + strand, within yecAat 1989.154 kb on - strand, within yecAat 1989.198 kb on + strand, within yecAat 1989.216 kb on - strand, within yecAat 1989.216 kb on - strand, within yecAat 1989.265 kb on + strand, within yecAat 1989.348 kb on + strand, within yecAat 1989.348 kb on + strand, within yecAat 1989.454 kb on + strand, within yecA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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1,988,066 - tyrP b1907 0.30 -1.7
1,988,183 + tyrP b1907 0.39 +0.2
1,988,309 - tyrP b1907 0.50 -1.7
1,988,344 - tyrP b1907 0.53 -2.2
1,988,420 + tyrP b1907 0.59 -1.7
1,988,420 + tyrP b1907 0.59 +4.1
1,988,478 + tyrP b1907 0.64 -2.3
1,988,706 - tyrP b1907 0.83 -1.1
1,988,860 + +1.3
1,988,898 + +1.4
1,988,957 - -0.6
1,989,096 + yecA b1908 0.18 -1.1
1,989,154 - yecA b1908 0.26 +1.0
1,989,198 + yecA b1908 0.33 -1.1
1,989,216 - yecA b1908 0.36 -0.7
1,989,216 - yecA b1908 0.36 -2.8
1,989,265 + yecA b1908 0.43 +0.2
1,989,348 + yecA b1908 0.56 -1.7
1,989,348 + yecA b1908 0.56 +0.3
1,989,454 + yecA b1908 0.71 +0.7

Or see this region's nucleotide sequence