Strain Fitness in Escherichia coli BW25113 around b1748

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastA and astC overlap by 4 nucleotidesastC and xthA are separated by 445 nucleotides b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789 astA b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006 astC b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258 xthA Position (kb) 1828 1829 1830 1831Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1827.814 kb on + strandat 1827.814 kb on + strandat 1827.870 kb on - strand, within astAat 1827.875 kb on + strand, within astAat 1828.010 kb on - strand, within astAat 1828.010 kb on - strand, within astAat 1828.024 kb on - strand, within astAat 1828.076 kb on - strand, within astAat 1828.076 kb on - strand, within astAat 1828.129 kb on - strand, within astAat 1828.236 kb on + strand, within astAat 1828.424 kb on - strand, within astAat 1828.629 kb on + strand, within astAat 1828.765 kb on + strandat 1829.079 kb on + strand, within astCat 1829.172 kb on + strand, within astCat 1829.172 kb on + strand, within astCat 1829.304 kb on - strand, within astCat 1829.304 kb on - strand, within astCat 1829.492 kb on - strand, within astCat 1829.492 kb on - strand, within astCat 1829.911 kb on - strandat 1829.911 kb on - strandat 1829.941 kb on - strandat 1830.075 kb on + strandat 1830.078 kb on + strandat 1830.078 kb on + strandat 1830.093 kb on - strandat 1830.238 kb on - strandat 1830.238 kb on - strandat 1830.375 kb on + strandat 1830.422 kb on - strandat 1830.640 kb on - strand, within xthAat 1830.904 kb on + strand, within xthAat 1830.904 kb on + strand, within xthAat 1830.979 kb on + strand, within xthA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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1,827,814 + -1.3
1,827,814 + +1.8
1,827,870 - astA b1747 0.11 +0.4
1,827,875 + astA b1747 0.12 -1.5
1,828,010 - astA b1747 0.25 +1.8
1,828,010 - astA b1747 0.25 +2.7
1,828,024 - astA b1747 0.26 +0.6
1,828,076 - astA b1747 0.31 +2.3
1,828,076 - astA b1747 0.31 +1.4
1,828,129 - astA b1747 0.36 +0.4
1,828,236 + astA b1747 0.46 +2.5
1,828,424 - astA b1747 0.65 -0.7
1,828,629 + astA b1747 0.84 +1.8
1,828,765 + +1.2
1,829,079 + astC b1748 0.24 -1.9
1,829,172 + astC b1748 0.32 -1.3
1,829,172 + astC b1748 0.32 +3.1
1,829,304 - astC b1748 0.42 -2.1
1,829,304 - astC b1748 0.42 +1.2
1,829,492 - astC b1748 0.58 -1.3
1,829,492 - astC b1748 0.58 -2.3
1,829,911 - -2.2
1,829,911 - -1.0
1,829,941 - +0.7
1,830,075 + -1.6
1,830,078 + -3.0
1,830,078 + -0.2
1,830,093 - +0.1
1,830,238 - +1.8
1,830,238 - -1.2
1,830,375 + +0.2
1,830,422 - +0.0
1,830,640 - xthA b1749 0.23 -1.0
1,830,904 + xthA b1749 0.56 +1.8
1,830,904 + xthA b1749 0.56 +0.8
1,830,979 + xthA b1749 0.65 +0.5

Or see this region's nucleotide sequence