Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tppB and gst are separated by 105 nucleotides gst and pdxY are separated by 43 nucleotides pdxY and tyrS are separated by 58 nucleotides
b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295
tppB
b1635: gst - glutathionine S-transferase (NCBI), at 1,712,401 to 1,713,006
gst
b1636: pdxY - pyridoxine kinase (NCBI), at 1,713,050 to 1,713,913
pdxY
b1637: tyrS - tyrosyl-tRNA synthetase (NCBI), at 1,713,972 to 1,715,246
tyrS
Position (kb)
1712
1713
1714 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1711.402 kb on + strand, within tppB at 1711.402 kb on + strand, within tppB at 1711.536 kb on + strand, within tppB at 1711.536 kb on + strand, within tppB at 1711.582 kb on - strand, within tppB at 1711.803 kb on - strand, within tppB at 1711.932 kb on - strand, within tppB at 1711.978 kb on - strand, within tppB at 1712.070 kb on - strand, within tppB at 1712.104 kb on - strand, within tppB at 1712.104 kb on - strand, within tppB at 1712.109 kb on + strand, within tppB at 1712.543 kb on - strand, within gst at 1712.623 kb on - strand, within gst at 1712.715 kb on + strand, within gst at 1713.214 kb on + strand, within pdxY at 1713.223 kb on + strand, within pdxY at 1713.223 kb on + strand, within pdxY at 1713.223 kb on + strand, within pdxY at 1713.352 kb on - strand, within pdxY at 1713.469 kb on + strand, within pdxY at 1713.546 kb on - strand, within pdxY at 1713.571 kb on + strand, within pdxY at 1713.571 kb on + strand, within pdxY at 1713.624 kb on - strand, within pdxY at 1713.889 kb on + strand at 1713.889 kb on + strand at 1713.913 kb on + strand at 1713.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,711,402 + tppB b1634 0.41 +0.0 1,711,402 + tppB b1634 0.41 -1.7 1,711,536 + tppB b1634 0.49 -0.4 1,711,536 + tppB b1634 0.49 -1.2 1,711,582 - tppB b1634 0.52 +0.6 1,711,803 - tppB b1634 0.67 -0.4 1,711,932 - tppB b1634 0.76 -1.2 1,711,978 - tppB b1634 0.79 +1.6 1,712,070 - tppB b1634 0.85 -1.4 1,712,104 - tppB b1634 0.87 +0.0 1,712,104 - tppB b1634 0.87 +1.6 1,712,109 + tppB b1634 0.88 -1.6 1,712,543 - gst b1635 0.23 +1.2 1,712,623 - gst b1635 0.37 +2.1 1,712,715 + gst b1635 0.52 -0.2 1,713,214 + pdxY b1636 0.19 -0.8 1,713,223 + pdxY b1636 0.20 +0.6 1,713,223 + pdxY b1636 0.20 -1.0 1,713,223 + pdxY b1636 0.20 -1.0 1,713,352 - pdxY b1636 0.35 +3.1 1,713,469 + pdxY b1636 0.48 -0.4 1,713,546 - pdxY b1636 0.57 -0.4 1,713,571 + pdxY b1636 0.60 +0.2 1,713,571 + pdxY b1636 0.60 -1.2 1,713,624 - pdxY b1636 0.66 +0.2 1,713,889 + -0.7 1,713,889 + -1.5 1,713,913 + +1.2 1,713,913 + -1.5
Or see this region's nucleotide sequence