Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaH and paaI overlap by 11 nucleotides paaI and paaJ overlap by 1 nucleotides paaJ and paaK are separated by 26 nucleotides
b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505
paaH
b1396: paaI - predicted thioesterase (NCBI), at 1,458,495 to 1,458,917
paaI
b1397: paaJ - acetyl-CoA acetyltransferase (NCBI), at 1,458,917 to 1,460,122
paaJ
b1398: paaK - phenylacetyl-CoA ligase (NCBI), at 1,460,149 to 1,461,462
paaK
Position (kb)
1458
1459
1460
1461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1457.921 kb on - strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.941 kb on + strand, within paaH at 1457.979 kb on + strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.104 kb on - strand, within paaH at 1458.104 kb on - strand, within paaH at 1458.138 kb on + strand, within paaH at 1458.207 kb on + strand, within paaH at 1458.487 kb on - strand at 1458.499 kb on + strand at 1458.558 kb on - strand, within paaI at 1458.590 kb on - strand, within paaI at 1458.590 kb on - strand, within paaI at 1458.602 kb on + strand, within paaI at 1458.602 kb on + strand, within paaI at 1458.679 kb on - strand, within paaI at 1458.689 kb on - strand, within paaI at 1458.786 kb on + strand, within paaI at 1458.909 kb on - strand at 1458.909 kb on - strand at 1458.914 kb on - strand at 1458.914 kb on - strand at 1459.088 kb on + strand, within paaJ at 1459.188 kb on + strand, within paaJ at 1459.188 kb on + strand, within paaJ at 1459.425 kb on + strand, within paaJ at 1459.425 kb on + strand, within paaJ at 1459.608 kb on + strand, within paaJ at 1459.647 kb on + strand at 1459.698 kb on + strand, within paaJ at 1459.778 kb on - strand, within paaJ at 1459.803 kb on + strand, within paaJ at 1459.882 kb on + strand, within paaJ at 1459.898 kb on + strand, within paaJ at 1459.916 kb on - strand, within paaJ at 1459.976 kb on + strand, within paaJ at 1460.080 kb on - strand at 1460.092 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,457,921 - paaH b1395 0.59 +0.2 1,457,921 - paaH b1395 0.59 -0.9 1,457,941 + paaH b1395 0.60 +1.1 1,457,979 + paaH b1395 0.63 +2.3 1,458,061 - paaH b1395 0.69 +3.5 1,458,061 - paaH b1395 0.69 +3.1 1,458,104 - paaH b1395 0.72 +0.1 1,458,104 - paaH b1395 0.72 -0.1 1,458,138 + paaH b1395 0.74 -1.9 1,458,207 + paaH b1395 0.79 +1.6 1,458,487 - +0.2 1,458,499 + +0.1 1,458,558 - paaI b1396 0.15 +3.1 1,458,590 - paaI b1396 0.22 +2.8 1,458,590 - paaI b1396 0.22 +0.2 1,458,602 + paaI b1396 0.25 -0.4 1,458,602 + paaI b1396 0.25 +0.9 1,458,679 - paaI b1396 0.43 -1.0 1,458,689 - paaI b1396 0.46 +0.4 1,458,786 + paaI b1396 0.69 -0.6 1,458,909 - -2.1 1,458,909 - +0.9 1,458,914 - -1.0 1,458,914 - -0.0 1,459,088 + paaJ b1397 0.14 -0.5 1,459,188 + paaJ b1397 0.22 +2.0 1,459,188 + paaJ b1397 0.22 -0.3 1,459,425 + paaJ b1397 0.42 -1.7 1,459,425 + paaJ b1397 0.42 -0.7 1,459,608 + paaJ b1397 0.57 -1.2 1,459,647 + +1.7 1,459,698 + paaJ b1397 0.65 -0.1 1,459,778 - paaJ b1397 0.71 -3.4 1,459,803 + paaJ b1397 0.73 +1.1 1,459,882 + paaJ b1397 0.80 +0.1 1,459,898 + paaJ b1397 0.81 -1.7 1,459,916 - paaJ b1397 0.83 -1.5 1,459,976 + paaJ b1397 0.88 -1.6 1,460,080 - -2.7 1,460,092 + +2.5
Or see this region's nucleotide sequence