Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hlyE and umuD are separated by 372 nucleotides umuD and umuC overlap by 1 nucleotides
b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617
hlyE
b1183: umuD - DNA polymerase V, subunit D (NCBI), at 1,229,990 to 1,230,409
umuD
b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677
umuC
Position (kb)
1229
1230
1231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1229.017 kb on - strand, within hlyE at 1229.017 kb on - strand, within hlyE at 1229.079 kb on - strand, within hlyE at 1229.256 kb on + strand, within hlyE at 1229.669 kb on - strand at 1229.703 kb on - strand at 1229.704 kb on - strand at 1229.718 kb on - strand at 1229.718 kb on - strand at 1229.838 kb on - strand at 1229.840 kb on - strand at 1229.842 kb on - strand at 1229.842 kb on - strand at 1229.844 kb on - strand at 1229.851 kb on - strand at 1229.851 kb on - strand at 1229.853 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.861 kb on + strand at 1229.897 kb on - strand at 1229.897 kb on - strand at 1230.140 kb on + strand, within umuD at 1230.307 kb on + strand, within umuD at 1230.446 kb on + strand at 1230.893 kb on + strand, within umuC at 1230.956 kb on + strand, within umuC at 1231.013 kb on - strand, within umuC at 1231.029 kb on + strand, within umuC at 1231.038 kb on - strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.208 kb on + strand at 1231.208 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.320 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.382 kb on - strand, within umuC
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,229,017 - hlyE b1182 0.34 +1.8 1,229,017 - hlyE b1182 0.34 +1.8 1,229,079 - hlyE b1182 0.41 +1.8 1,229,256 + hlyE b1182 0.60 +1.8 1,229,669 - +1.8 1,229,703 - -0.8 1,229,704 - -0.2 1,229,718 - +1.8 1,229,718 - +0.8 1,229,838 - -2.1 1,229,840 - +0.3 1,229,842 - -0.2 1,229,842 - -1.6 1,229,844 - +0.2 1,229,851 - +4.3 1,229,851 - +1.3 1,229,853 - +1.3 1,229,859 - -0.3 1,229,859 - -0.6 1,229,859 - +1.1 1,229,861 + +1.8 1,229,897 - -0.8 1,229,897 - +0.2 1,230,140 + umuD b1183 0.36 +1.7 1,230,307 + umuD b1183 0.75 +1.8 1,230,446 + -0.4 1,230,893 + umuC b1184 0.38 -1.6 1,230,956 + umuC b1184 0.43 -0.6 1,231,013 - umuC b1184 0.48 +0.2 1,231,029 + umuC b1184 0.49 +0.3 1,231,038 - umuC b1184 0.50 -2.2 1,231,109 + umuC b1184 0.55 -1.6 1,231,109 + umuC b1184 0.55 -0.2 1,231,208 + +0.2 1,231,208 + umuC b1184 0.63 -2.9 1,231,270 + umuC b1184 0.68 -1.1 1,231,270 + umuC b1184 0.68 +2.0 1,231,320 + umuC b1184 0.72 +2.7 1,231,359 + umuC b1184 0.75 +1.3 1,231,359 + umuC b1184 0.75 +0.2 1,231,382 - umuC b1184 0.77 -0.2
Or see this region's nucleotide sequence