Strain Fitness in Escherichia coli BW25113 around b1115

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmfd and ycfT are separated by 127 nucleotidesycfT and lolC are separated by 261 nucleotides b1114: mfd - transcription-repair coupling factor (NCBI), at 1,169,741 to 1,173,187 mfd b1115: ycfT - predicted inner membrane protein (NCBI), at 1,173,315 to 1,174,388 ycfT b1116: lolC - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,174,650 to 1,175,849 lolC Position (kb) 1173 1174 1175Strain fitness (log2 ratio) -2 -1 0 1 2at 1172.574 kb on - strand, within mfdat 1172.758 kb on - strand, within mfdat 1172.772 kb on + strand, within mfdat 1172.872 kb on + strandat 1172.925 kb on + strandat 1172.941 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.071 kb on + strandat 1173.089 kb on + strandat 1173.148 kb on - strandat 1173.173 kb on - strandat 1173.192 kb on + strandat 1173.218 kb on - strandat 1173.463 kb on - strand, within ycfTat 1173.506 kb on - strand, within ycfTat 1173.506 kb on - strand, within ycfTat 1173.541 kb on - strand, within ycfTat 1173.593 kb on + strand, within ycfTat 1173.602 kb on - strand, within ycfTat 1173.602 kb on - strand, within ycfTat 1173.710 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.747 kb on + strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.767 kb on - strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.111 kb on + strand, within ycfTat 1174.176 kb on - strand, within ycfTat 1174.176 kb on - strand, within ycfTat 1174.267 kb on + strand, within ycfTat 1174.457 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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1,172,574 - mfd b1114 0.82 +0.7
1,172,758 - mfd b1114 0.88 -0.6
1,172,772 + mfd b1114 0.88 -1.8
1,172,872 + -1.4
1,172,925 + +0.8
1,172,941 + +0.4
1,173,058 + -0.4
1,173,058 + -0.1
1,173,058 + +0.5
1,173,058 + +1.0
1,173,071 + -2.5
1,173,089 + -0.4
1,173,148 - -1.1
1,173,173 - -1.0
1,173,192 + +0.2
1,173,218 - +1.9
1,173,463 - ycfT b1115 0.14 -1.4
1,173,506 - ycfT b1115 0.18 +1.4
1,173,506 - ycfT b1115 0.18 -1.6
1,173,541 - ycfT b1115 0.21 +0.2
1,173,593 + ycfT b1115 0.26 -1.7
1,173,602 - ycfT b1115 0.27 +0.9
1,173,602 - ycfT b1115 0.27 -0.1
1,173,710 + ycfT b1115 0.37 -1.6
1,173,720 + ycfT b1115 0.38 +0.4
1,173,720 + ycfT b1115 0.38 -0.5
1,173,747 + ycfT b1115 0.40 -0.8
1,173,757 - ycfT b1115 0.41 -1.4
1,173,757 - ycfT b1115 0.41 -0.8
1,173,767 - ycfT b1115 0.42 -2.0
1,173,791 + ycfT b1115 0.44 +0.9
1,173,791 + ycfT b1115 0.44 -1.1
1,174,091 - ycfT b1115 0.72 +0.7
1,174,091 - ycfT b1115 0.72 +0.6
1,174,111 + ycfT b1115 0.74 -1.3
1,174,176 - ycfT b1115 0.80 +1.0
1,174,176 - ycfT b1115 0.80 -2.0
1,174,267 + ycfT b1115 0.89 -0.1
1,174,457 + -0.3

Or see this region's nucleotide sequence