Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdG and nfnB are separated by 107 nucleotides nfnB and ybdF are separated by 93 nucleotides ybdF and ybdJ are separated by 64 nucleotides ybdJ and ybdK are separated by 65 nucleotides
b0577: ybdG - predicted mechanosensitive channel (NCBI), at 602,639 to 603,886
ybdG
b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647
nfnB
b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109
ybdF
b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422
ybdJ
b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606
ybdK
Position (kb) 603
604
605 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 603.065 kb on + strand, within ybdG at 603.079 kb on + strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.088 kb on - strand, within ybdG at 603.088 kb on - strand, within ybdG at 603.107 kb on - strand, within ybdG at 603.107 kb on - strand, within ybdG at 603.130 kb on - strand, within ybdG at 603.130 kb on - strand, within ybdG at 603.134 kb on + strand, within ybdG at 603.163 kb on + strand, within ybdG at 603.164 kb on + strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.196 kb on - strand, within ybdG at 603.196 kb on - strand, within ybdG at 603.197 kb on - strand, within ybdG at 603.197 kb on - strand, within ybdG at 603.225 kb on - strand, within ybdG at 603.277 kb on + strand, within ybdG at 603.301 kb on + strand, within ybdG at 603.312 kb on - strand, within ybdG at 603.347 kb on + strand, within ybdG at 603.355 kb on - strand, within ybdG at 603.369 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.380 kb on + strand, within ybdG at 603.402 kb on + strand, within ybdG at 603.402 kb on + strand, within ybdG at 603.403 kb on - strand, within ybdG at 603.403 kb on - strand, within ybdG at 603.408 kb on - strand, within ybdG at 603.417 kb on - strand, within ybdG at 603.436 kb on + strand, within ybdG at 603.444 kb on - strand, within ybdG at 603.446 kb on + strand, within ybdG at 603.457 kb on - strand, within ybdG at 603.479 kb on - strand, within ybdG at 603.528 kb on + strand, within ybdG at 603.538 kb on - strand, within ybdG at 603.540 kb on - strand, within ybdG at 603.540 kb on - strand, within ybdG at 603.542 kb on + strand, within ybdG at 603.551 kb on - strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.558 kb on - strand, within ybdG at 603.558 kb on - strand, within ybdG at 603.558 kb on - strand, within ybdG at 603.560 kb on + strand, within ybdG at 603.560 kb on + strand, within ybdG at 603.560 kb on + strand, within ybdG at 603.561 kb on + strand, within ybdG at 603.561 kb on - strand, within ybdG at 603.561 kb on - strand, within ybdG at 603.564 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.567 kb on + strand, within ybdG at 603.568 kb on - strand, within ybdG at 603.572 kb on + strand, within ybdG at 603.572 kb on + strand, within ybdG at 603.575 kb on - strand, within ybdG at 603.577 kb on + strand, within ybdG at 603.577 kb on + strand, within ybdG at 603.577 kb on - strand, within ybdG at 603.580 kb on - strand, within ybdG at 603.580 kb on - strand, within ybdG at 603.580 kb on - strand, within ybdG at 603.582 kb on + strand, within ybdG at 603.582 kb on - strand, within ybdG at 603.594 kb on - strand, within ybdG at 603.656 kb on + strand, within ybdG at 603.656 kb on + strand, within ybdG at 603.659 kb on - strand, within ybdG at 603.659 kb on - strand, within ybdG at 603.663 kb on - strand, within ybdG at 603.663 kb on - strand, within ybdG at 603.714 kb on + strand, within ybdG at 603.728 kb on - strand, within ybdG at 603.728 kb on - strand, within ybdG at 603.762 kb on - strand at 603.762 kb on - strand at 603.762 kb on - strand at 603.769 kb on + strand at 603.769 kb on + strand at 603.779 kb on - strand at 603.825 kb on + strand at 603.828 kb on - strand at 603.828 kb on - strand at 603.830 kb on + strand at 603.830 kb on + strand at 603.830 kb on + strand at 603.831 kb on + strand at 603.838 kb on - strand at 603.838 kb on - strand at 603.839 kb on - strand at 603.842 kb on + strand at 603.842 kb on + strand at 603.857 kb on + strand at 603.857 kb on + strand at 603.858 kb on - strand at 603.864 kb on + strand at 603.864 kb on + strand at 603.881 kb on - strand at 603.881 kb on - strand at 603.897 kb on + strand at 603.897 kb on + strand at 603.899 kb on + strand at 603.899 kb on + strand at 603.900 kb on + strand at 603.904 kb on - strand at 603.905 kb on - strand at 603.905 kb on - strand at 603.905 kb on - strand at 603.907 kb on - strand at 603.910 kb on + strand at 603.910 kb on + strand at 603.910 kb on + strand at 603.910 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.918 kb on - strand at 603.922 kb on + strand at 603.940 kb on - strand at 603.940 kb on - strand at 603.978 kb on - strand at 603.991 kb on + strand at 604.002 kb on + strand at 604.031 kb on + strand at 604.034 kb on - strand at 604.034 kb on - strand at 604.039 kb on - strand at 604.074 kb on - strand, within nfnB at 604.084 kb on - strand, within nfnB at 604.097 kb on + strand, within nfnB at 604.097 kb on + strand, within nfnB at 604.210 kb on + strand, within nfnB at 604.210 kb on + strand, within nfnB at 604.249 kb on + strand, within nfnB at 604.347 kb on + strand, within nfnB at 604.404 kb on - strand, within nfnB at 604.425 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.538 kb on + strand, within nfnB at 604.540 kb on + strand, within nfnB at 604.777 kb on - strand at 604.871 kb on + strand, within ybdF at 604.888 kb on - strand, within ybdF at 604.894 kb on - strand, within ybdF at 605.086 kb on + strand at 605.180 kb on - strand at 605.180 kb on - strand at 605.335 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.472 kb on - strand at 605.498 kb on + strand at 605.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 603,065 + ybdG b0577 0.34 +0.6 603,079 + ybdG b0577 0.35 +0.6 603,084 - ybdG b0577 0.36 +1.5 603,084 - ybdG b0577 0.36 +0.0 603,084 - ybdG b0577 0.36 +0.3 603,084 - ybdG b0577 0.36 +2.6 603,084 - ybdG b0577 0.36 +0.4 603,084 - ybdG b0577 0.36 +1.6 603,084 - ybdG b0577 0.36 -2.3 603,088 - ybdG b0577 0.36 -3.0 603,088 - ybdG b0577 0.36 +1.8 603,107 - ybdG b0577 0.38 +0.8 603,107 - ybdG b0577 0.38 -2.5 603,130 - ybdG b0577 0.39 -2.2 603,130 - ybdG b0577 0.39 -3.8 603,134 + ybdG b0577 0.40 +3.1 603,163 + ybdG b0577 0.42 -0.4 603,164 + ybdG b0577 0.42 +0.0 603,172 - ybdG b0577 0.43 +0.5 603,172 - ybdG b0577 0.43 +3.2 603,172 - ybdG b0577 0.43 -4.3 603,172 - ybdG b0577 0.43 +0.0 603,172 - ybdG b0577 0.43 -1.7 603,172 - ybdG b0577 0.43 +0.3 603,196 - ybdG b0577 0.45 +0.8 603,196 - ybdG b0577 0.45 -2.3 603,197 - ybdG b0577 0.45 -2.6 603,197 - ybdG b0577 0.45 -1.3 603,225 - ybdG b0577 0.47 +0.8 603,277 + ybdG b0577 0.51 +1.2 603,301 + ybdG b0577 0.53 -0.4 603,312 - ybdG b0577 0.54 +0.6 603,347 + ybdG b0577 0.57 +1.2 603,355 - ybdG b0577 0.57 -1.2 603,369 - ybdG b0577 0.58 -1.1 603,375 - ybdG b0577 0.59 -0.1 603,375 - ybdG b0577 0.59 -1.0 603,375 - ybdG b0577 0.59 -1.2 603,375 - ybdG b0577 0.59 -1.2 603,375 - ybdG b0577 0.59 -3.1 603,380 + ybdG b0577 0.59 +1.6 603,402 + ybdG b0577 0.61 -1.6 603,402 + ybdG b0577 0.61 -1.6 603,403 - ybdG b0577 0.61 +1.5 603,403 - ybdG b0577 0.61 -2.1 603,408 - ybdG b0577 0.62 -2.7 603,417 - ybdG b0577 0.62 +0.7 603,436 + ybdG b0577 0.64 -2.1 603,444 - ybdG b0577 0.65 -2.4 603,446 + ybdG b0577 0.65 +0.6 603,457 - ybdG b0577 0.66 -0.3 603,479 - ybdG b0577 0.67 +0.5 603,528 + ybdG b0577 0.71 +0.0 603,538 - ybdG b0577 0.72 +0.2 603,540 - ybdG b0577 0.72 +0.5 603,540 - ybdG b0577 0.72 -0.4 603,542 + ybdG b0577 0.72 +0.0 603,551 - ybdG b0577 0.73 -1.2 603,553 + ybdG b0577 0.73 +0.2 603,553 + ybdG b0577 0.73 -0.7 603,553 + ybdG b0577 0.73 +2.8 603,558 - ybdG b0577 0.74 -0.4 603,558 - ybdG b0577 0.74 -0.7 603,558 - ybdG b0577 0.74 -2.4 603,560 + ybdG b0577 0.74 -1.7 603,560 + ybdG b0577 0.74 -1.8 603,560 + ybdG b0577 0.74 +0.0 603,561 + ybdG b0577 0.74 -1.3 603,561 - ybdG b0577 0.74 -0.6 603,561 - ybdG b0577 0.74 +0.0 603,564 + ybdG b0577 0.74 +2.0 603,567 + ybdG b0577 0.74 -0.5 603,567 + ybdG b0577 0.74 -1.2 603,567 + ybdG b0577 0.74 -1.0 603,567 + ybdG b0577 0.74 -0.5 603,567 + ybdG b0577 0.74 -1.6 603,567 + ybdG b0577 0.74 +0.0 603,568 - ybdG b0577 0.74 +1.9 603,572 + ybdG b0577 0.75 +0.1 603,572 + ybdG b0577 0.75 +2.1 603,575 - ybdG b0577 0.75 -2.3 603,577 + ybdG b0577 0.75 -0.1 603,577 + ybdG b0577 0.75 -1.4 603,577 - ybdG b0577 0.75 -1.7 603,580 - ybdG b0577 0.75 -2.7 603,580 - ybdG b0577 0.75 -1.5 603,580 - ybdG b0577 0.75 -1.4 603,582 + ybdG b0577 0.76 -2.1 603,582 - ybdG b0577 0.76 +0.8 603,594 - ybdG b0577 0.77 -0.5 603,656 + ybdG b0577 0.81 -0.4 603,656 + ybdG b0577 0.81 -0.7 603,659 - ybdG b0577 0.82 -0.2 603,659 - ybdG b0577 0.82 -1.4 603,663 - ybdG b0577 0.82 +0.6 603,663 - ybdG b0577 0.82 -0.4 603,714 + ybdG b0577 0.86 +1.6 603,728 - ybdG b0577 0.87 +2.4 603,728 - ybdG b0577 0.87 -1.6 603,762 - +0.6 603,762 - -2.1 603,762 - -1.2 603,769 + -1.2 603,769 + -1.2 603,779 - -1.7 603,825 + -1.5 603,828 - -1.7 603,828 - -3.0 603,830 + +0.1 603,830 + +0.6 603,830 + -2.1 603,831 + +1.1 603,838 - -1.9 603,838 - -2.2 603,839 - -1.2 603,842 + -0.1 603,842 + -0.7 603,857 + -2.6 603,857 + +1.5 603,858 - +0.3 603,864 + +1.8 603,864 + -1.4 603,881 - +0.3 603,881 - -0.9 603,897 + +0.5 603,897 + -0.1 603,899 + -0.5 603,899 + -0.5 603,900 + -0.8 603,904 - -2.3 603,905 - -0.3 603,905 - -2.7 603,905 - +0.9 603,907 - +0.4 603,910 + -0.1 603,910 + +2.7 603,910 + -1.5 603,910 - -0.7 603,918 - -1.9 603,918 - +0.3 603,918 - +1.1 603,918 - +0.4 603,918 - +0.7 603,918 - +0.6 603,918 - +1.1 603,922 + -2.5 603,940 - -1.5 603,940 - +0.5 603,978 - +0.1 603,991 + +0.5 604,002 + -0.7 604,031 + -0.4 604,034 - -0.5 604,034 - +0.1 604,039 - +0.9 604,074 - nfnB b0578 0.12 -0.1 604,084 - nfnB b0578 0.14 -0.0 604,097 + nfnB b0578 0.16 -0.1 604,097 + nfnB b0578 0.16 -1.2 604,210 + nfnB b0578 0.33 -0.8 604,210 + nfnB b0578 0.33 +2.2 604,249 + nfnB b0578 0.39 +0.5 604,347 + nfnB b0578 0.54 +2.6 604,404 - nfnB b0578 0.63 -2.2 604,425 - nfnB b0578 0.66 +1.1 604,488 - nfnB b0578 0.76 -1.5 604,488 - nfnB b0578 0.76 -0.5 604,488 - nfnB b0578 0.76 -2.2 604,538 + nfnB b0578 0.83 -0.4 604,540 + nfnB b0578 0.83 -0.1 604,777 - +1.4 604,871 + ybdF b0579 0.35 -1.7 604,888 - ybdF b0579 0.40 -0.3 604,894 - ybdF b0579 0.41 -1.1 605,086 + +0.9 605,180 - -2.0 605,180 - -1.8 605,335 + ybdJ b0580 0.65 +2.1 605,355 + ybdJ b0580 0.73 -1.2 605,355 + ybdJ b0580 0.73 +0.0 605,472 - -2.1 605,498 + -3.0 605,549 - +1.6
Or see this region's nucleotide sequence