Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemH and aes overlap by 4 nucleotides aes and gsk are separated by 151 nucleotides
b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241
hemH
b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197
aes
b0477: gsk - inosine/guanosine kinase (NCBI), at 499,349 to 500,653
gsk
Position (kb) 498
499
500 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 498.393 kb on + strand, within aes at 498.393 kb on + strand, within aes at 498.461 kb on + strand, within aes at 498.464 kb on + strand, within aes at 498.476 kb on + strand, within aes at 498.490 kb on + strand, within aes at 498.531 kb on - strand, within aes at 498.531 kb on - strand, within aes at 498.546 kb on + strand, within aes at 498.546 kb on + strand, within aes at 498.564 kb on - strand, within aes at 498.564 kb on - strand, within aes at 498.624 kb on - strand, within aes at 498.675 kb on - strand, within aes at 498.730 kb on - strand, within aes at 498.740 kb on + strand, within aes at 498.839 kb on - strand, within aes at 498.894 kb on - strand, within aes at 498.942 kb on + strand, within aes at 498.960 kb on - strand, within aes at 499.132 kb on + strand at 499.138 kb on - strand at 499.285 kb on - strand at 499.453 kb on + strand at 499.457 kb on - strand at 499.526 kb on - strand, within gsk at 499.550 kb on + strand, within gsk at 499.558 kb on + strand, within gsk at 499.631 kb on + strand, within gsk at 499.631 kb on + strand, within gsk at 499.639 kb on + strand, within gsk at 499.656 kb on - strand, within gsk at 499.656 kb on - strand, within gsk at 499.656 kb on - strand, within gsk at 499.803 kb on + strand, within gsk at 499.803 kb on + strand, within gsk at 499.856 kb on - strand, within gsk at 499.913 kb on + strand, within gsk at 499.913 kb on + strand, within gsk at 499.951 kb on + strand, within gsk at 499.952 kb on + strand, within gsk at 500.002 kb on + strand, within gsk at 500.027 kb on - strand, within gsk at 500.072 kb on - strand, within gsk at 500.168 kb on + strand, within gsk at 500.176 kb on - strand, within gsk at 500.186 kb on - strand, within gsk
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 498,393 + aes b0476 0.16 -2.5 498,393 + aes b0476 0.16 -0.7 498,461 + aes b0476 0.23 -2.4 498,464 + aes b0476 0.24 +0.9 498,476 + aes b0476 0.25 -1.6 498,490 + aes b0476 0.26 +1.4 498,531 - aes b0476 0.31 -1.4 498,531 - aes b0476 0.31 +1.6 498,546 + aes b0476 0.32 -2.0 498,546 + aes b0476 0.32 -0.7 498,564 - aes b0476 0.34 +2.6 498,564 - aes b0476 0.34 +1.5 498,624 - aes b0476 0.40 +0.5 498,675 - aes b0476 0.46 -2.0 498,730 - aes b0476 0.51 -1.9 498,740 + aes b0476 0.52 -2.4 498,839 - aes b0476 0.63 -3.4 498,894 - aes b0476 0.68 +1.0 498,942 + aes b0476 0.73 +0.5 498,960 - aes b0476 0.75 -2.8 499,132 + -1.6 499,138 - -0.3 499,285 - -0.1 499,453 + -2.6 499,457 - +0.3 499,526 - gsk b0477 0.14 -1.8 499,550 + gsk b0477 0.15 +1.5 499,558 + gsk b0477 0.16 +0.5 499,631 + gsk b0477 0.22 +2.8 499,631 + gsk b0477 0.22 -0.7 499,639 + gsk b0477 0.22 -2.3 499,656 - gsk b0477 0.24 -1.2 499,656 - gsk b0477 0.24 -0.1 499,656 - gsk b0477 0.24 +0.7 499,803 + gsk b0477 0.35 -1.0 499,803 + gsk b0477 0.35 +0.0 499,856 - gsk b0477 0.39 -1.6 499,913 + gsk b0477 0.43 +2.6 499,913 + gsk b0477 0.43 +2.6 499,951 + gsk b0477 0.46 +0.3 499,952 + gsk b0477 0.46 +1.1 500,002 + gsk b0477 0.50 +0.7 500,027 - gsk b0477 0.52 +1.2 500,072 - gsk b0477 0.55 +2.9 500,168 + gsk b0477 0.63 +1.6 500,176 - gsk b0477 0.63 +0.0 500,186 - gsk b0477 0.64 -1.6
Or see this region's nucleotide sequence