Strain Fitness in Escherichia coli BW25113 around b0475

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntadk and hemH are separated by 235 nucleotideshemH and aes overlap by 4 nucleotides b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197 aes Position (kb) 497 498 499Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 498.393 kb on + strand, within aesat 498.393 kb on + strand, within aesat 498.461 kb on + strand, within aesat 498.464 kb on + strand, within aesat 498.476 kb on + strand, within aesat 498.490 kb on + strand, within aesat 498.531 kb on - strand, within aesat 498.531 kb on - strand, within aesat 498.546 kb on + strand, within aesat 498.546 kb on + strand, within aesat 498.564 kb on - strand, within aesat 498.564 kb on - strand, within aesat 498.624 kb on - strand, within aesat 498.675 kb on - strand, within aesat 498.730 kb on - strand, within aesat 498.740 kb on + strand, within aesat 498.839 kb on - strand, within aesat 498.894 kb on - strand, within aesat 498.942 kb on + strand, within aesat 498.960 kb on - strand, within aesat 499.132 kb on + strandat 499.138 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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498,393 + aes b0476 0.16 -2.5
498,393 + aes b0476 0.16 -0.7
498,461 + aes b0476 0.23 -2.4
498,464 + aes b0476 0.24 +0.9
498,476 + aes b0476 0.25 -1.6
498,490 + aes b0476 0.26 +1.4
498,531 - aes b0476 0.31 -1.4
498,531 - aes b0476 0.31 +1.6
498,546 + aes b0476 0.32 -2.0
498,546 + aes b0476 0.32 -0.7
498,564 - aes b0476 0.34 +2.6
498,564 - aes b0476 0.34 +1.5
498,624 - aes b0476 0.40 +0.5
498,675 - aes b0476 0.46 -2.0
498,730 - aes b0476 0.51 -1.9
498,740 + aes b0476 0.52 -2.4
498,839 - aes b0476 0.63 -3.4
498,894 - aes b0476 0.68 +1.0
498,942 + aes b0476 0.73 +0.5
498,960 - aes b0476 0.75 -2.8
499,132 + -1.6
499,138 - -0.3

Or see this region's nucleotide sequence