Strain Fitness in Escherichia coli BW25113 around b3826

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpldB and yigL are separated by 7 nucleotidesyigL and yigM are separated by 75 nucleotidesyigM and metR overlap by 113 nucleotides b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215 pldB b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023 yigL b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998 yigM b3828: metR - DNA-binding transcriptional activator, homocysteine-binding (NCBI), at 4,009,886 to 4,010,839 metR Position (kb) 4008 4009 4010Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4007.264 kb on - strandat 4007.264 kb on - strandat 4007.289 kb on - strandat 4007.289 kb on - strandat 4007.319 kb on - strand, within pldBat 4007.345 kb on + strand, within pldBat 4007.345 kb on + strand, within pldBat 4007.354 kb on - strand, within pldBat 4007.357 kb on + strand, within pldBat 4007.357 kb on + strand, within pldBat 4007.361 kb on + strand, within pldBat 4007.364 kb on - strand, within pldBat 4007.366 kb on + strand, within pldBat 4007.379 kb on - strand, within pldBat 4007.380 kb on + strand, within pldBat 4007.382 kb on + strand, within pldBat 4007.411 kb on + strand, within pldBat 4007.421 kb on - strand, within pldBat 4007.421 kb on - strand, within pldBat 4007.483 kb on + strand, within pldBat 4007.483 kb on + strand, within pldBat 4007.491 kb on - strand, within pldBat 4007.518 kb on - strand, within pldBat 4007.526 kb on - strand, within pldBat 4007.559 kb on + strand, within pldBat 4007.559 kb on + strand, within pldBat 4007.564 kb on + strand, within pldBat 4007.565 kb on + strand, within pldBat 4007.590 kb on + strand, within pldBat 4007.614 kb on - strand, within pldBat 4007.652 kb on + strand, within pldBat 4007.667 kb on + strand, within pldBat 4007.667 kb on + strand, within pldBat 4007.731 kb on + strand, within pldBat 4007.731 kb on + strand, within pldBat 4007.749 kb on - strand, within pldBat 4007.749 kb on - strand, within pldBat 4007.811 kb on + strand, within pldBat 4007.811 kb on + strand, within pldBat 4007.861 kb on + strand, within pldBat 4007.869 kb on - strand, within pldBat 4007.869 kb on - strand, within pldBat 4007.871 kb on - strand, within pldBat 4007.875 kb on + strand, within pldBat 4007.875 kb on + strand, within pldBat 4007.881 kb on - strand, within pldBat 4007.903 kb on - strand, within pldBat 4008.002 kb on - strand, within pldBat 4008.002 kb on - strand, within pldBat 4008.017 kb on + strand, within pldBat 4008.017 kb on - strand, within pldBat 4008.025 kb on - strand, within pldBat 4008.041 kb on + strand, within pldBat 4008.054 kb on - strand, within pldBat 4008.060 kb on - strand, within pldBat 4008.066 kb on + strand, within pldBat 4008.086 kb on + strand, within pldBat 4008.086 kb on + strand, within pldBat 4008.225 kb on + strandat 4008.230 kb on + strandat 4008.230 kb on + strandat 4008.302 kb on + strandat 4008.302 kb on + strandat 4008.310 kb on - strand, within yigLat 4008.350 kb on + strand, within yigLat 4008.350 kb on + strand, within yigLat 4008.393 kb on - strand, within yigLat 4008.416 kb on + strand, within yigLat 4008.430 kb on + strand, within yigLat 4008.489 kb on - strand, within yigLat 4008.495 kb on + strand, within yigLat 4008.496 kb on - strand, within yigLat 4008.505 kb on + strand, within yigLat 4008.526 kb on - strand, within yigLat 4008.526 kb on - strand, within yigLat 4008.529 kb on + strand, within yigLat 4008.572 kb on + strand, within yigLat 4008.572 kb on + strand, within yigLat 4008.621 kb on + strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.632 kb on + strand, within yigLat 4008.677 kb on + strand, within yigLat 4008.677 kb on + strand, within yigLat 4008.680 kb on + strand, within yigLat 4008.688 kb on - strand, within yigLat 4008.708 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.762 kb on - strand, within yigLat 4008.762 kb on - strand, within yigLat 4008.903 kb on - strand, within yigLat 4008.910 kb on + strand, within yigLat 4008.918 kb on - strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.968 kb on + strandat 4009.067 kb on - strandat 4009.082 kb on - strandat 4009.134 kb on - strandat 4009.176 kb on - strandat 4009.176 kb on - strandat 4009.205 kb on - strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.283 kb on - strand, within yigMat 4009.283 kb on - strand, within yigMat 4009.311 kb on - strand, within yigMat 4009.334 kb on + strand, within yigMat 4009.334 kb on + strand, within yigMat 4009.338 kb on - strand, within yigMat 4009.348 kb on - strand, within yigMat 4009.348 kb on - strand, within yigMat 4009.406 kb on - strand, within yigMat 4009.428 kb on + strand, within yigMat 4009.434 kb on + strand, within yigMat 4009.434 kb on + strand, within yigMat 4009.451 kb on - strand, within yigMat 4009.495 kb on - strand, within yigMat 4009.495 kb on - strand, within yigMat 4009.506 kb on + strand, within yigMat 4009.524 kb on - strand, within yigMat 4009.600 kb on + strand, within yigMat 4009.772 kb on + strand, within yigMat 4009.839 kb on - strand, within yigMat 4009.839 kb on - strand, within yigMat 4009.845 kb on - strand, within yigMat 4009.845 kb on - strand, within yigMat 4009.874 kb on + strand, within yigMat 4009.904 kb on + strandat 4009.917 kb on - strandat 4009.921 kb on - strandat 4009.921 kb on - strandat 4009.947 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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4,007,264 - -1.6
4,007,264 - -1.4
4,007,289 - +1.0
4,007,289 - +0.5
4,007,319 - pldB b3825 0.12 -2.6
4,007,345 + pldB b3825 0.15 -3.0
4,007,345 + pldB b3825 0.15 +1.2
4,007,354 - pldB b3825 0.16 -0.7
4,007,357 + pldB b3825 0.16 -2.4
4,007,357 + pldB b3825 0.16 -1.8
4,007,361 + pldB b3825 0.16 +1.3
4,007,364 - pldB b3825 0.17 -3.2
4,007,366 + pldB b3825 0.17 -2.9
4,007,379 - pldB b3825 0.18 +1.3
4,007,380 + pldB b3825 0.18 -0.7
4,007,382 + pldB b3825 0.18 -0.3
4,007,411 + pldB b3825 0.21 -0.3
4,007,421 - pldB b3825 0.22 +0.3
4,007,421 - pldB b3825 0.22 +1.0
4,007,483 + pldB b3825 0.28 +0.8
4,007,483 + pldB b3825 0.28 +0.0
4,007,491 - pldB b3825 0.29 +1.0
4,007,518 - pldB b3825 0.32 -2.3
4,007,526 - pldB b3825 0.33 -0.5
4,007,559 + pldB b3825 0.36 -2.1
4,007,559 + pldB b3825 0.36 +1.5
4,007,564 + pldB b3825 0.36 +0.1
4,007,565 + pldB b3825 0.36 -1.3
4,007,590 + pldB b3825 0.39 +0.4
4,007,614 - pldB b3825 0.41 +0.9
4,007,652 + pldB b3825 0.45 +0.4
4,007,667 + pldB b3825 0.46 -0.4
4,007,667 + pldB b3825 0.46 +0.2
4,007,731 + pldB b3825 0.53 +1.3
4,007,731 + pldB b3825 0.53 -2.5
4,007,749 - pldB b3825 0.54 -0.6
4,007,749 - pldB b3825 0.54 +1.2
4,007,811 + pldB b3825 0.60 +0.4
4,007,811 + pldB b3825 0.60 +1.9
4,007,861 + pldB b3825 0.65 -1.1
4,007,869 - pldB b3825 0.66 +1.4
4,007,869 - pldB b3825 0.66 -2.0
4,007,871 - pldB b3825 0.66 +0.4
4,007,875 + pldB b3825 0.67 -2.8
4,007,875 + pldB b3825 0.67 +0.4
4,007,881 - pldB b3825 0.67 -1.4
4,007,903 - pldB b3825 0.69 -0.8
4,008,002 - pldB b3825 0.79 -0.9
4,008,002 - pldB b3825 0.79 +0.7
4,008,017 + pldB b3825 0.81 -1.4
4,008,017 - pldB b3825 0.81 +0.7
4,008,025 - pldB b3825 0.81 -0.2
4,008,041 + pldB b3825 0.83 -1.8
4,008,054 - pldB b3825 0.84 -0.6
4,008,060 - pldB b3825 0.85 -1.2
4,008,066 + pldB b3825 0.85 +0.6
4,008,086 + pldB b3825 0.87 +0.8
4,008,086 + pldB b3825 0.87 +3.6
4,008,225 + -1.8
4,008,230 + -0.5
4,008,230 + -0.5
4,008,302 + +2.6
4,008,302 + +0.4
4,008,310 - yigL b3826 0.11 -2.9
4,008,350 + yigL b3826 0.16 -0.5
4,008,350 + yigL b3826 0.16 +1.2
4,008,393 - yigL b3826 0.21 -0.8
4,008,416 + yigL b3826 0.24 -0.5
4,008,430 + yigL b3826 0.26 +0.7
4,008,489 - yigL b3826 0.33 -0.6
4,008,495 + yigL b3826 0.34 -0.7
4,008,496 - yigL b3826 0.34 -2.3
4,008,505 + yigL b3826 0.35 -3.2
4,008,526 - yigL b3826 0.38 +0.3
4,008,526 - yigL b3826 0.38 +0.9
4,008,529 + yigL b3826 0.38 -3.2
4,008,572 + yigL b3826 0.44 +1.9
4,008,572 + yigL b3826 0.44 -1.2
4,008,621 + yigL b3826 0.50 +1.2
4,008,629 - yigL b3826 0.51 +2.1
4,008,629 - yigL b3826 0.51 -0.6
4,008,629 - yigL b3826 0.51 -1.4
4,008,632 + yigL b3826 0.51 +2.6
4,008,677 + yigL b3826 0.57 -0.3
4,008,677 + yigL b3826 0.57 +0.2
4,008,680 + yigL b3826 0.57 -0.2
4,008,688 - yigL b3826 0.58 -1.0
4,008,708 + yigL b3826 0.61 -4.1
4,008,754 + yigL b3826 0.66 +2.7
4,008,754 + yigL b3826 0.66 -2.9
4,008,754 + yigL b3826 0.66 -1.7
4,008,754 + yigL b3826 0.66 +2.3
4,008,762 - yigL b3826 0.67 -1.4
4,008,762 - yigL b3826 0.67 +1.1
4,008,903 - yigL b3826 0.85 -1.9
4,008,910 + yigL b3826 0.86 -0.8
4,008,918 - yigL b3826 0.87 -1.2
4,008,920 + yigL b3826 0.87 -2.6
4,008,920 + yigL b3826 0.87 -2.1
4,008,920 + yigL b3826 0.87 -2.1
4,008,968 + +2.5
4,009,067 - +0.9
4,009,082 - -0.5
4,009,134 - -2.2
4,009,176 - +0.3
4,009,176 - -4.4
4,009,205 - yigM b3827 0.12 -0.2
4,009,275 + yigM b3827 0.20 -3.3
4,009,275 + yigM b3827 0.20 +1.7
4,009,275 + yigM b3827 0.20 -1.4
4,009,275 + yigM b3827 0.20 +0.5
4,009,275 + yigM b3827 0.20 -0.6
4,009,275 + yigM b3827 0.20 +0.5
4,009,283 - yigM b3827 0.20 -1.4
4,009,283 - yigM b3827 0.20 -1.2
4,009,311 - yigM b3827 0.24 +1.6
4,009,334 + yigM b3827 0.26 +1.3
4,009,334 + yigM b3827 0.26 +2.8
4,009,338 - yigM b3827 0.27 -0.6
4,009,348 - yigM b3827 0.28 +0.6
4,009,348 - yigM b3827 0.28 +1.8
4,009,406 - yigM b3827 0.34 +1.3
4,009,428 + yigM b3827 0.37 +0.6
4,009,434 + yigM b3827 0.37 +2.4
4,009,434 + yigM b3827 0.37 -1.5
4,009,451 - yigM b3827 0.39 +0.8
4,009,495 - yigM b3827 0.44 +1.0
4,009,495 - yigM b3827 0.44 -1.6
4,009,506 + yigM b3827 0.45 -0.6
4,009,524 - yigM b3827 0.47 -0.6
4,009,600 + yigM b3827 0.56 +0.2
4,009,772 + yigM b3827 0.75 +0.9
4,009,839 - yigM b3827 0.82 +2.7
4,009,839 - yigM b3827 0.82 +0.1
4,009,845 - yigM b3827 0.83 -2.0
4,009,845 - yigM b3827 0.83 -1.8
4,009,874 + yigM b3827 0.86 -2.3
4,009,904 + -0.6
4,009,917 - +1.4
4,009,921 - +1.1
4,009,921 - -0.9
4,009,947 - +0.5

Or see this region's nucleotide sequence