Strain Fitness in Escherichia coli BW25113 around b2731

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypE and fhlA are separated by 73 nucleotidesfhlA and ygbA are separated by 36 nucleotidesygbA and mutS are separated by 286 nucleotides b2730: hypE - carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq), at 2,851,276 to 2,852,286 hypE b2731: fhlA - DNA-binding transcriptional activator (NCBI), at 2,852,360 to 2,854,438 fhlA b2732: ygbA - hypothetical protein (NCBI), at 2,854,475 to 2,854,828 ygbA b2733: mutS - DNA mismatch repair protein (NCBI), at 2,855,115 to 2,857,676 mutS Position (kb) 2852 2853 2854 2855Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2851.420 kb on - strand, within hypEat 2851.438 kb on - strand, within hypEat 2851.468 kb on + strand, within hypEat 2851.480 kb on + strand, within hypEat 2851.480 kb on + strand, within hypEat 2851.524 kb on - strand, within hypEat 2851.538 kb on + strand, within hypEat 2851.538 kb on + strand, within hypEat 2851.831 kb on - strand, within hypEat 2851.874 kb on + strand, within hypEat 2851.892 kb on - strand, within hypEat 2851.903 kb on + strand, within hypEat 2851.940 kb on - strand, within hypEat 2852.009 kb on - strand, within hypEat 2852.024 kb on + strand, within hypEat 2852.024 kb on + strand, within hypEat 2852.046 kb on - strand, within hypEat 2852.046 kb on - strand, within hypEat 2852.108 kb on - strand, within hypEat 2852.108 kb on - strand, within hypEat 2852.199 kb on - strandat 2852.199 kb on - strandat 2852.234 kb on - strandat 2852.234 kb on - strandat 2852.300 kb on + strandat 2852.300 kb on + strandat 2852.331 kb on + strandat 2852.331 kb on + strandat 2852.339 kb on - strandat 2852.364 kb on + strandat 2852.364 kb on + strandat 2852.428 kb on - strandat 2852.428 kb on - strandat 2852.457 kb on - strandat 2852.524 kb on + strandat 2852.612 kb on - strand, within fhlAat 2852.696 kb on - strand, within fhlAat 2852.796 kb on - strand, within fhlAat 2852.853 kb on + strand, within fhlAat 2852.866 kb on - strand, within fhlAat 2852.866 kb on - strand, within fhlAat 2852.896 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.962 kb on + strand, within fhlAat 2852.990 kb on - strand, within fhlAat 2853.030 kb on + strand, within fhlAat 2853.167 kb on - strand, within fhlAat 2853.183 kb on + strand, within fhlAat 2853.183 kb on + strand, within fhlAat 2853.230 kb on - strand, within fhlAat 2853.251 kb on + strand, within fhlAat 2853.298 kb on - strand, within fhlAat 2853.298 kb on - strand, within fhlAat 2853.462 kb on - strand, within fhlAat 2853.518 kb on + strand, within fhlAat 2853.526 kb on - strand, within fhlAat 2853.526 kb on - strand, within fhlAat 2853.869 kb on - strand, within fhlAat 2854.040 kb on - strand, within fhlAat 2854.040 kb on - strand, within fhlAat 2854.115 kb on + strand, within fhlAat 2854.129 kb on - strand, within fhlAat 2854.131 kb on + strand, within fhlAat 2854.192 kb on - strand, within fhlAat 2854.260 kb on + strandat 2854.282 kb on - strandat 2854.529 kb on - strand, within ygbAat 2854.552 kb on + strand, within ygbAat 2854.586 kb on - strand, within ygbAat 2854.622 kb on - strand, within ygbAat 2854.652 kb on - strand, within ygbAat 2854.652 kb on - strand, within ygbAat 2854.984 kb on + strandat 2854.984 kb on + strandat 2855.167 kb on - strandat 2855.314 kb on + strandat 2855.314 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,851,420 - hypE b2730 0.14 -3.6
2,851,438 - hypE b2730 0.16 +2.5
2,851,468 + hypE b2730 0.19 +1.0
2,851,480 + hypE b2730 0.20 -0.1
2,851,480 + hypE b2730 0.20 -1.7
2,851,524 - hypE b2730 0.25 -1.1
2,851,538 + hypE b2730 0.26 -1.8
2,851,538 + hypE b2730 0.26 -0.5
2,851,831 - hypE b2730 0.55 +1.5
2,851,874 + hypE b2730 0.59 +0.0
2,851,892 - hypE b2730 0.61 +0.3
2,851,903 + hypE b2730 0.62 -1.8
2,851,940 - hypE b2730 0.66 -3.8
2,852,009 - hypE b2730 0.73 -1.6
2,852,024 + hypE b2730 0.74 -2.4
2,852,024 + hypE b2730 0.74 -1.8
2,852,046 - hypE b2730 0.76 -0.3
2,852,046 - hypE b2730 0.76 -1.5
2,852,108 - hypE b2730 0.82 +2.2
2,852,108 - hypE b2730 0.82 -0.4
2,852,199 - +2.1
2,852,199 - -0.7
2,852,234 - +1.5
2,852,234 - -0.1
2,852,300 + -2.5
2,852,300 + -2.3
2,852,331 + -1.3
2,852,331 + -1.3
2,852,339 - +1.3
2,852,364 + -1.8
2,852,364 + +1.3
2,852,428 - +0.5
2,852,428 - +0.5
2,852,457 - -0.2
2,852,524 + -1.6
2,852,612 - fhlA b2731 0.12 +0.4
2,852,696 - fhlA b2731 0.16 -0.8
2,852,796 - fhlA b2731 0.21 -2.8
2,852,853 + fhlA b2731 0.24 -1.9
2,852,866 - fhlA b2731 0.24 +0.9
2,852,866 - fhlA b2731 0.24 -1.6
2,852,896 + fhlA b2731 0.26 -2.0
2,852,962 + fhlA b2731 0.29 -2.0
2,852,962 + fhlA b2731 0.29 -2.1
2,852,962 + fhlA b2731 0.29 -1.8
2,852,990 - fhlA b2731 0.30 -2.5
2,853,030 + fhlA b2731 0.32 +3.3
2,853,167 - fhlA b2731 0.39 -0.0
2,853,183 + fhlA b2731 0.40 -2.0
2,853,183 + fhlA b2731 0.40 -0.8
2,853,230 - fhlA b2731 0.42 -2.8
2,853,251 + fhlA b2731 0.43 -1.7
2,853,298 - fhlA b2731 0.45 +1.8
2,853,298 - fhlA b2731 0.45 -1.0
2,853,462 - fhlA b2731 0.53 +0.1
2,853,518 + fhlA b2731 0.56 +0.5
2,853,526 - fhlA b2731 0.56 -1.3
2,853,526 - fhlA b2731 0.56 -2.0
2,853,869 - fhlA b2731 0.73 +2.4
2,854,040 - fhlA b2731 0.81 -0.7
2,854,040 - fhlA b2731 0.81 -1.1
2,854,115 + fhlA b2731 0.84 -1.7
2,854,129 - fhlA b2731 0.85 +0.2
2,854,131 + fhlA b2731 0.85 -1.7
2,854,192 - fhlA b2731 0.88 +0.9
2,854,260 + -0.1
2,854,282 - -0.5
2,854,529 - ygbA b2732 0.15 +1.7
2,854,552 + ygbA b2732 0.22 +1.6
2,854,586 - ygbA b2732 0.31 -0.4
2,854,622 - ygbA b2732 0.42 -1.2
2,854,652 - ygbA b2732 0.50 +0.6
2,854,652 - ygbA b2732 0.50 -4.0
2,854,984 + +2.0
2,854,984 + +1.0
2,855,167 - +1.7
2,855,314 + +1.5
2,855,314 + -0.0

Or see this region's nucleotide sequence