Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt maeB and talA are separated by 288 nucleotides talA and tktB are separated by 19 nucleotides
b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399
maeB
b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638
talA
b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661
tktB
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2575.869 kb on + strand, within maeB at 2576.054 kb on + strand, within maeB at 2576.062 kb on - strand, within maeB at 2576.062 kb on - strand, within maeB at 2576.164 kb on + strand, within maeB at 2576.174 kb on - strand at 2576.281 kb on - strand at 2576.376 kb on + strand at 2576.391 kb on - strand at 2576.391 kb on - strand at 2576.462 kb on + strand at 2576.472 kb on + strand at 2576.480 kb on - strand at 2576.480 kb on - strand at 2576.586 kb on - strand at 2576.853 kb on - strand, within talA at 2576.984 kb on - strand, within talA at 2577.042 kb on - strand, within talA at 2577.044 kb on + strand, within talA at 2577.044 kb on + strand, within talA at 2577.060 kb on - strand, within talA at 2577.060 kb on - strand, within talA at 2577.263 kb on + strand, within talA at 2577.266 kb on + strand, within talA at 2577.491 kb on - strand, within talA at 2577.659 kb on - strand at 2577.696 kb on + strand at 2577.704 kb on - strand at 2577.706 kb on - strand at 2577.706 kb on - strand at 2577.856 kb on - strand at 2577.856 kb on - strand at 2578.022 kb on + strand, within tktB at 2578.083 kb on - strand, within tktB at 2578.083 kb on - strand, within tktB at 2578.169 kb on + strand, within tktB at 2578.352 kb on + strand, within tktB at 2578.372 kb on + strand, within tktB at 2578.439 kb on + strand, within tktB at 2578.439 kb on + strand, within tktB at 2578.624 kb on + strand, within tktB
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 2,575,869 + maeB b2463 0.77 -0.3 2,576,054 + maeB b2463 0.85 -0.5 2,576,062 - maeB b2463 0.85 +1.1 2,576,062 - maeB b2463 0.85 -1.0 2,576,164 + maeB b2463 0.90 +1.7 2,576,174 - +1.0 2,576,281 - +1.3 2,576,376 + +0.1 2,576,391 - -2.7 2,576,391 - -1.5 2,576,462 + +0.0 2,576,472 + +0.3 2,576,480 - +2.3 2,576,480 - -2.8 2,576,586 - -0.4 2,576,853 - talA b2464 0.17 -0.9 2,576,984 - talA b2464 0.31 -0.2 2,577,042 - talA b2464 0.37 -0.7 2,577,044 + talA b2464 0.37 +0.9 2,577,044 + talA b2464 0.37 -0.7 2,577,060 - talA b2464 0.39 -1.7 2,577,060 - talA b2464 0.39 +1.7 2,577,263 + talA b2464 0.60 -1.8 2,577,266 + talA b2464 0.61 +0.6 2,577,491 - talA b2464 0.84 -1.1 2,577,659 - -0.5 2,577,696 + -1.2 2,577,704 - +0.3 2,577,706 - +0.3 2,577,706 - -1.3 2,577,856 - +0.7 2,577,856 - +0.9 2,578,022 + tktB b2465 0.18 +0.9 2,578,083 - tktB b2465 0.21 -1.8 2,578,083 - tktB b2465 0.21 +2.5 2,578,169 + tktB b2465 0.25 +2.2 2,578,352 + tktB b2465 0.35 -1.5 2,578,372 + tktB b2465 0.36 +0.9 2,578,439 + tktB b2465 0.39 +0.7 2,578,439 + tktB b2465 0.39 +0.5 2,578,624 + tktB b2465 0.48 -0.8
Or see this region's nucleotide sequence