Strain Fitness in Escherichia coli BW25113 around b2429

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeU and murP are separated by 3 nucleotidesmurP and b2430 are separated by 4 nucleotides b2428: yfeU - N-acetylmuramic acid-6-phosphate etherase (NCBI), at 2,543,795 to 2,544,691 yfeU b2429: murP - N-acetylmuramic acid phosphotransfer permease (NCBI), at 2,544,695 to 2,546,119 murP b2430: b2430 - putative beta-lactamase (VIMSS), at 2,546,124 to 2,547,428 b2430 Position (kb) 2544 2545 2546 2547Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2543.880 kb on - strandat 2543.935 kb on - strand, within yfeUat 2544.000 kb on + strand, within yfeUat 2544.058 kb on - strand, within yfeUat 2544.130 kb on - strand, within yfeUat 2544.130 kb on - strand, within yfeUat 2544.390 kb on - strand, within yfeUat 2544.425 kb on + strand, within yfeUat 2544.425 kb on + strand, within yfeUat 2544.686 kb on + strandat 2544.693 kb on - strandat 2544.704 kb on - strandat 2544.717 kb on - strandat 2544.727 kb on + strandat 2544.801 kb on + strandat 2544.801 kb on + strandat 2544.811 kb on - strandat 2544.811 kb on - strandat 2544.964 kb on - strand, within murPat 2545.000 kb on - strand, within murPat 2545.234 kb on - strand, within murPat 2545.234 kb on - strand, within murPat 2545.234 kb on - strand, within murPat 2545.236 kb on - strand, within murPat 2545.306 kb on - strand, within murPat 2545.347 kb on + strand, within murPat 2545.445 kb on - strand, within murPat 2545.471 kb on - strand, within murPat 2545.471 kb on - strand, within murPat 2545.494 kb on - strand, within murPat 2545.494 kb on - strand, within murPat 2545.559 kb on + strand, within murPat 2545.559 kb on + strand, within murPat 2545.620 kb on + strand, within murPat 2545.637 kb on + strand, within murPat 2545.761 kb on - strand, within murPat 2545.768 kb on + strand, within murPat 2545.768 kb on + strand, within murPat 2545.786 kb on - strand, within murPat 2545.886 kb on + strand, within murPat 2546.146 kb on - strandat 2546.233 kb on - strandat 2546.239 kb on + strandat 2546.412 kb on + strand, within b2430at 2546.420 kb on - strand, within b2430at 2546.420 kb on - strand, within b2430at 2546.423 kb on + strand, within b2430at 2546.489 kb on - strand, within b2430at 2546.489 kb on - strand, within b2430at 2546.663 kb on - strand, within b2430at 2546.674 kb on + strand, within b2430at 2546.758 kb on + strand, within b2430at 2546.844 kb on + strand, within b2430at 2546.969 kb on - strand, within b2430at 2546.969 kb on - strand, within b2430at 2547.030 kb on + strand, within b2430at 2547.062 kb on - strand, within b2430at 2547.062 kb on - strand, within b2430at 2547.071 kb on - strand, within b2430at 2547.087 kb on - strand, within b2430

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,543,880 - +1.3
2,543,935 - yfeU b2428 0.16 -2.2
2,544,000 + yfeU b2428 0.23 -1.8
2,544,058 - yfeU b2428 0.29 -1.1
2,544,130 - yfeU b2428 0.37 -0.1
2,544,130 - yfeU b2428 0.37 -2.3
2,544,390 - yfeU b2428 0.66 +2.4
2,544,425 + yfeU b2428 0.70 +0.1
2,544,425 + yfeU b2428 0.70 +0.8
2,544,686 + +0.4
2,544,693 - -0.2
2,544,704 - +2.2
2,544,717 - -0.6
2,544,727 + -1.9
2,544,801 + -0.0
2,544,801 + -0.9
2,544,811 - +1.3
2,544,811 - +0.5
2,544,964 - murP b2429 0.19 -2.6
2,545,000 - murP b2429 0.21 -0.1
2,545,234 - murP b2429 0.38 +0.9
2,545,234 - murP b2429 0.38 +1.2
2,545,234 - murP b2429 0.38 -1.1
2,545,236 - murP b2429 0.38 -1.6
2,545,306 - murP b2429 0.43 -1.1
2,545,347 + murP b2429 0.46 -1.2
2,545,445 - murP b2429 0.53 -0.8
2,545,471 - murP b2429 0.54 +0.7
2,545,471 - murP b2429 0.54 -1.1
2,545,494 - murP b2429 0.56 +0.8
2,545,494 - murP b2429 0.56 +0.5
2,545,559 + murP b2429 0.61 -1.3
2,545,559 + murP b2429 0.61 -1.4
2,545,620 + murP b2429 0.65 +1.4
2,545,637 + murP b2429 0.66 -0.7
2,545,761 - murP b2429 0.75 -2.5
2,545,768 + murP b2429 0.75 -1.0
2,545,768 + murP b2429 0.75 -1.4
2,545,786 - murP b2429 0.77 -0.3
2,545,886 + murP b2429 0.84 +1.0
2,546,146 - +2.2
2,546,233 - -1.2
2,546,239 + +0.9
2,546,412 + b2430 b2430 0.22 -3.4
2,546,420 - b2430 b2430 0.23 -0.2
2,546,420 - b2430 b2430 0.23 +2.1
2,546,423 + b2430 b2430 0.23 +0.7
2,546,489 - b2430 b2430 0.28 -2.8
2,546,489 - b2430 b2430 0.28 +1.5
2,546,663 - b2430 b2430 0.41 -2.8
2,546,674 + b2430 b2430 0.42 +1.4
2,546,758 + b2430 b2430 0.49 +0.9
2,546,844 + b2430 b2430 0.55 +2.1
2,546,969 - b2430 b2430 0.65 +1.7
2,546,969 - b2430 b2430 0.65 -0.7
2,547,030 + b2430 b2430 0.69 -2.0
2,547,062 - b2430 b2430 0.72 +0.9
2,547,062 - b2430 b2430 0.72 -2.9
2,547,071 - b2430 b2430 0.73 +0.8
2,547,087 - b2430 b2430 0.74 +1.4

Or see this region's nucleotide sequence