Strain Fitness in Escherichia coli BW25113 around b2222

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntatoC and atoD are separated by 195 nucleotidesatoD and atoA overlap by 1 nucleotidesatoA and atoE overlap by 4 nucleotides b2220: atoC - fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI), at 2,319,888 to 2,321,273 atoC b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131 atoD b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781 atoA b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100 atoE Position (kb) 2322 2323Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2321.179 kb on - strandat 2321.194 kb on - strandat 2321.196 kb on + strandat 2321.196 kb on + strandat 2321.236 kb on - strandat 2321.261 kb on + strandat 2321.266 kb on + strandat 2321.496 kb on - strandat 2321.519 kb on - strandat 2321.700 kb on + strand, within atoDat 2321.707 kb on - strand, within atoDat 2321.818 kb on + strand, within atoDat 2321.967 kb on + strand, within atoDat 2322.085 kb on - strandat 2322.255 kb on - strand, within atoAat 2322.362 kb on - strand, within atoAat 2322.391 kb on + strand, within atoAat 2322.410 kb on - strand, within atoAat 2322.423 kb on - strand, within atoAat 2322.629 kb on - strand, within atoAat 2322.892 kb on + strandat 2322.927 kb on + strand, within atoEat 2322.978 kb on - strand, within atoEat 2322.978 kb on - strand, within atoEat 2322.999 kb on + strand, within atoEat 2323.113 kb on - strand, within atoEat 2323.139 kb on + strand, within atoEat 2323.194 kb on - strand, within atoEat 2323.260 kb on + strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.403 kb on + strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.426 kb on - strand, within atoEat 2323.475 kb on - strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.608 kb on + strand, within atoE

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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2,321,179 - +2.4
2,321,194 - +0.7
2,321,196 + -2.2
2,321,196 + +0.5
2,321,236 - -3.0
2,321,261 + -1.3
2,321,266 + +0.7
2,321,496 - +0.8
2,321,519 - -0.2
2,321,700 + atoD b2221 0.35 -2.6
2,321,707 - atoD b2221 0.36 -1.6
2,321,818 + atoD b2221 0.53 +0.6
2,321,967 + atoD b2221 0.75 +0.0
2,322,085 - -2.1
2,322,255 - atoA b2222 0.19 +0.2
2,322,362 - atoA b2222 0.35 +0.1
2,322,391 + atoA b2222 0.40 -2.9
2,322,410 - atoA b2222 0.43 -1.5
2,322,423 - atoA b2222 0.45 -1.8
2,322,629 - atoA b2222 0.76 +0.7
2,322,892 + -2.5
2,322,927 + atoE b2223 0.11 +1.1
2,322,978 - atoE b2223 0.15 -0.3
2,322,978 - atoE b2223 0.15 +3.3
2,322,999 + atoE b2223 0.17 -2.6
2,323,113 - atoE b2223 0.25 -0.3
2,323,139 + atoE b2223 0.27 +0.2
2,323,194 - atoE b2223 0.31 +0.4
2,323,260 + atoE b2223 0.36 -2.3
2,323,321 - atoE b2223 0.41 -1.4
2,323,321 - atoE b2223 0.41 -0.8
2,323,403 + atoE b2223 0.47 +0.4
2,323,417 - atoE b2223 0.48 +1.1
2,323,417 - atoE b2223 0.48 -0.9
2,323,426 - atoE b2223 0.49 -1.0
2,323,475 - atoE b2223 0.53 -2.7
2,323,601 + atoE b2223 0.62 +2.6
2,323,601 + atoE b2223 0.62 +1.6
2,323,608 + atoE b2223 0.63 -1.1

Or see this region's nucleotide sequence