Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaJ and cpsG are separated by 54 nucleotides cpsG and cpsB are separated by 104 nucleotides
b2047: wcaJ - predicted UDP-glucose lipid carrier transferase (NCBI), at 2,118,184 to 2,119,578
wcaJ
b2048: cpsG - phosphomannomutase (NCBI), at 2,119,633 to 2,121,003
cpsG
b2049: cpsB - mannose-1-phosphate guanyltransferase (NCBI), at 2,121,108 to 2,122,544
cpsB
Position (kb)
2119
2120
2121
2122 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2119.085 kb on - strand, within wcaJ at 2119.106 kb on - strand, within wcaJ at 2119.436 kb on - strand, within wcaJ at 2119.436 kb on - strand, within wcaJ at 2119.439 kb on + strand at 2119.439 kb on + strand at 2119.476 kb on + strand at 2119.520 kb on + strand at 2119.536 kb on - strand at 2119.599 kb on + strand at 2119.796 kb on + strand, within cpsG at 2119.959 kb on + strand, within cpsG at 2120.189 kb on + strand, within cpsG at 2120.628 kb on - strand, within cpsG at 2120.645 kb on - strand, within cpsG at 2120.645 kb on - strand, within cpsG at 2120.689 kb on + strand, within cpsG at 2120.759 kb on + strand, within cpsG at 2120.759 kb on + strand, within cpsG at 2120.782 kb on - strand, within cpsG at 2120.814 kb on + strand, within cpsG at 2120.823 kb on - strand, within cpsG at 2120.880 kb on - strand at 2120.880 kb on - strand at 2121.026 kb on + strand at 2121.026 kb on + strand at 2121.027 kb on + strand at 2121.354 kb on + strand, within cpsB at 2121.354 kb on + strand, within cpsB at 2121.452 kb on + strand, within cpsB at 2121.452 kb on + strand, within cpsB at 2121.504 kb on + strand, within cpsB at 2121.605 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.969 kb on + strand, within cpsB
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 2,119,085 - wcaJ b2047 0.65 -0.6 2,119,106 - wcaJ b2047 0.66 -1.6 2,119,436 - wcaJ b2047 0.90 -1.7 2,119,436 - wcaJ b2047 0.90 -0.6 2,119,439 + -0.7 2,119,439 + -1.7 2,119,476 + +1.1 2,119,520 + -3.3 2,119,536 - +1.5 2,119,599 + +0.2 2,119,796 + cpsG b2048 0.12 +0.4 2,119,959 + cpsG b2048 0.24 +0.4 2,120,189 + cpsG b2048 0.41 -1.8 2,120,628 - cpsG b2048 0.73 -1.2 2,120,645 - cpsG b2048 0.74 -1.4 2,120,645 - cpsG b2048 0.74 +1.2 2,120,689 + cpsG b2048 0.77 -2.6 2,120,759 + cpsG b2048 0.82 -1.1 2,120,759 + cpsG b2048 0.82 -0.2 2,120,782 - cpsG b2048 0.84 -1.8 2,120,814 + cpsG b2048 0.86 -2.2 2,120,823 - cpsG b2048 0.87 +1.0 2,120,880 - +0.1 2,120,880 - -0.9 2,121,026 + -1.2 2,121,026 + -0.1 2,121,027 + -2.8 2,121,354 + cpsB b2049 0.17 -1.2 2,121,354 + cpsB b2049 0.17 +1.3 2,121,452 + cpsB b2049 0.24 -0.7 2,121,452 + cpsB b2049 0.24 +1.8 2,121,504 + cpsB b2049 0.28 -1.9 2,121,605 + cpsB b2049 0.35 +0.7 2,121,742 + cpsB b2049 0.44 -1.1 2,121,742 + cpsB b2049 0.44 -1.1 2,121,969 + cpsB b2049 0.60 +3.0
Or see this region's nucleotide sequence