Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliN overlap by 4 nucleotides fliN and fliO are separated by 2 nucleotides fliO and fliP overlap by 1 nucleotides
b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115
fliM
b1946: fliN - flagellar motor switch protein (NCBI), at 2,019,112 to 2,019,525
fliN
b1947: fliO - flagellar biosynthesis (VIMSS), at 2,019,528 to 2,019,893
fliO
b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630
fliP
Position (kb)
2019
2020 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2018.299 kb on + strand, within fliM at 2018.411 kb on + strand, within fliM at 2018.420 kb on - strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.652 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.700 kb on + strand, within fliM at 2018.870 kb on + strand, within fliM at 2018.875 kb on + strand, within fliM at 2018.890 kb on - strand, within fliM at 2019.025 kb on + strand at 2019.106 kb on + strand at 2019.397 kb on + strand, within fliN at 2019.437 kb on + strand, within fliN at 2019.538 kb on + strand at 2019.538 kb on + strand at 2019.995 kb on + strand, within fliP at 2020.000 kb on + strand, within fliP at 2020.231 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.375 kb on + strand, within fliP at 2020.382 kb on - strand, within fliP
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 2,018,299 + fliM b1945 0.19 +1.0 2,018,411 + fliM b1945 0.30 +0.5 2,018,420 - fliM b1945 0.31 -0.2 2,018,466 + fliM b1945 0.35 -3.5 2,018,466 + fliM b1945 0.35 -2.6 2,018,608 - fliM b1945 0.49 -1.7 2,018,608 - fliM b1945 0.49 -0.8 2,018,610 - fliM b1945 0.50 -0.2 2,018,610 - fliM b1945 0.50 -1.6 2,018,652 - fliM b1945 0.54 -2.1 2,018,689 - fliM b1945 0.58 +1.3 2,018,689 - fliM b1945 0.58 -2.6 2,018,700 + fliM b1945 0.59 -0.4 2,018,870 + fliM b1945 0.76 -3.4 2,018,875 + fliM b1945 0.76 +2.0 2,018,890 - fliM b1945 0.78 -0.1 2,019,025 + +1.5 2,019,106 + -0.9 2,019,397 + fliN b1946 0.69 -0.7 2,019,437 + fliN b1946 0.79 -0.4 2,019,538 + -1.5 2,019,538 + +2.0 2,019,995 + fliP b1948 0.14 +0.2 2,020,000 + fliP b1948 0.14 -0.6 2,020,231 - fliP b1948 0.46 +2.4 2,020,359 - fliP b1948 0.63 +0.3 2,020,359 - fliP b1948 0.63 -1.4 2,020,375 + fliP b1948 0.65 +0.8 2,020,382 - fliP b1948 0.66 +0.7
Or see this region's nucleotide sequence