Strain Fitness in Escherichia coli BW25113 around b1886

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttap and tar are separated by 45 nucleotidestar and cheW are separated by 144 nucleotidescheW and cheA are separated by 20 nucleotides b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008 tap b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715 tar b1887: cheW - purine-binding chemotaxis protein (NCBI), at 1,970,860 to 1,971,363 cheW b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348 cheA Position (kb) 1969 1970 1971Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1968.203 kb on + strand, within tapat 1968.218 kb on + strand, within tapat 1968.238 kb on - strand, within tapat 1968.316 kb on + strand, within tapat 1968.329 kb on - strand, within tapat 1968.571 kb on - strand, within tapat 1968.651 kb on + strand, within tapat 1968.651 kb on + strand, within tapat 1968.660 kb on + strand, within tapat 1968.786 kb on - strand, within tapat 1968.786 kb on - strand, within tapat 1968.828 kb on - strand, within tapat 1968.852 kb on + strandat 1969.099 kb on + strandat 1969.099 kb on + strandat 1969.425 kb on - strand, within tarat 1969.705 kb on + strand, within tarat 1969.705 kb on + strand, within tarat 1969.713 kb on - strand, within tarat 1969.833 kb on + strand, within tarat 1969.925 kb on - strand, within tarat 1969.957 kb on + strand, within tarat 1970.104 kb on + strand, within tarat 1970.121 kb on - strand, within tarat 1970.185 kb on - strand, within tarat 1970.212 kb on + strand, within tarat 1970.217 kb on - strand, within tarat 1970.217 kb on - strand, within tarat 1970.267 kb on + strand, within tarat 1970.267 kb on + strand, within tarat 1970.292 kb on - strand, within tarat 1970.292 kb on - strand, within tarat 1970.319 kb on + strand, within tarat 1970.369 kb on + strand, within tarat 1970.369 kb on + strand, within tarat 1970.390 kb on - strand, within tarat 1970.488 kb on - strand, within tarat 1970.489 kb on + strand, within tarat 1970.497 kb on + strand, within tarat 1970.502 kb on + strand, within tarat 1970.602 kb on + strandat 1970.656 kb on - strandat 1970.666 kb on + strandat 1970.676 kb on + strandat 1970.765 kb on - strandat 1970.884 kb on + strandat 1970.989 kb on - strand, within cheWat 1971.037 kb on - strand, within cheWat 1971.075 kb on + strand, within cheWat 1971.116 kb on + strand, within cheWat 1971.129 kb on - strand, within cheWat 1971.140 kb on - strand, within cheWat 1971.165 kb on + strand, within cheWat 1971.165 kb on + strand, within cheWat 1971.173 kb on - strand, within cheWat 1971.247 kb on + strand, within cheWat 1971.247 kb on + strand, within cheWat 1971.250 kb on + strand, within cheWat 1971.251 kb on + strand, within cheWat 1971.251 kb on + strand, within cheWat 1971.272 kb on + strand, within cheWat 1971.275 kb on - strand, within cheWat 1971.539 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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1,968,203 + tap b1885 0.50 +1.3
1,968,218 + tap b1885 0.51 -0.3
1,968,238 - tap b1885 0.52 +0.4
1,968,316 + tap b1885 0.57 -1.7
1,968,329 - tap b1885 0.58 -0.3
1,968,571 - tap b1885 0.73 +1.7
1,968,651 + tap b1885 0.78 -1.4
1,968,651 + tap b1885 0.78 -2.4
1,968,660 + tap b1885 0.78 -2.8
1,968,786 - tap b1885 0.86 +1.0
1,968,786 - tap b1885 0.86 -1.2
1,968,828 - tap b1885 0.89 -0.1
1,968,852 + -3.3
1,969,099 + +1.4
1,969,099 + -0.2
1,969,425 - tar b1886 0.22 +0.9
1,969,705 + tar b1886 0.39 +0.4
1,969,705 + tar b1886 0.39 -2.6
1,969,713 - tar b1886 0.40 +0.6
1,969,833 + tar b1886 0.47 +0.3
1,969,925 - tar b1886 0.52 +1.8
1,969,957 + tar b1886 0.54 +0.9
1,970,104 + tar b1886 0.63 +0.4
1,970,121 - tar b1886 0.64 +2.7
1,970,185 - tar b1886 0.68 +0.2
1,970,212 + tar b1886 0.70 +3.1
1,970,217 - tar b1886 0.70 -0.2
1,970,217 - tar b1886 0.70 -0.8
1,970,267 + tar b1886 0.73 +3.3
1,970,267 + tar b1886 0.73 -0.7
1,970,292 - tar b1886 0.74 -0.9
1,970,292 - tar b1886 0.74 -1.9
1,970,319 + tar b1886 0.76 +0.2
1,970,369 + tar b1886 0.79 +1.0
1,970,369 + tar b1886 0.79 +1.4
1,970,390 - tar b1886 0.80 +2.4
1,970,488 - tar b1886 0.86 +0.1
1,970,489 + tar b1886 0.86 -1.5
1,970,497 + tar b1886 0.87 -1.9
1,970,502 + tar b1886 0.87 -0.6
1,970,602 + -1.5
1,970,656 - +2.3
1,970,666 + -0.9
1,970,676 + -0.2
1,970,765 - -2.1
1,970,884 + +1.8
1,970,989 - cheW b1887 0.26 -1.8
1,971,037 - cheW b1887 0.35 -2.1
1,971,075 + cheW b1887 0.43 -1.7
1,971,116 + cheW b1887 0.51 -4.6
1,971,129 - cheW b1887 0.53 -2.8
1,971,140 - cheW b1887 0.56 +1.7
1,971,165 + cheW b1887 0.61 -0.6
1,971,165 + cheW b1887 0.61 +1.8
1,971,173 - cheW b1887 0.62 -0.1
1,971,247 + cheW b1887 0.77 -0.9
1,971,247 + cheW b1887 0.77 -3.6
1,971,250 + cheW b1887 0.77 +0.2
1,971,251 + cheW b1887 0.78 -0.4
1,971,251 + cheW b1887 0.78 -2.3
1,971,272 + cheW b1887 0.82 +1.5
1,971,275 - cheW b1887 0.82 -0.1
1,971,539 + -0.6

Or see this region's nucleotide sequence