Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheZ and cheY are separated by 10 nucleotides cheY and cheB are separated by 14 nucleotides cheB and cheR are separated by 2 nucleotides cheR and tap are separated by 18 nucleotides
b1881: cheZ - chemotaxis regulator, protein phosphatase for CheY (NCBI), at 1,964,417 to 1,965,061
cheZ
b1882: cheY - chemotaxis regulator transmitting signal to flagellar motor component (NCBI), at 1,965,072 to 1,965,461
cheY
b1883: cheB - chemotaxis-specific methylesterase (NCBI), at 1,965,476 to 1,966,525
cheB
b1884: cheR - chemotaxis regulator, protein-glutamate methyltransferase (NCBI), at 1,966,528 to 1,967,388
cheR
b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008
tap
Position (kb) 1965
1966
1967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1964.507 kb on - strand, within cheZ at 1964.609 kb on - strand, within cheZ at 1964.847 kb on - strand, within cheZ at 1964.889 kb on + strand, within cheZ at 1965.270 kb on + strand, within cheY at 1965.696 kb on - strand, within cheB at 1965.815 kb on - strand, within cheB at 1966.252 kb on + strand, within cheB at 1966.273 kb on + strand, within cheB at 1966.356 kb on - strand, within cheB at 1966.514 kb on + strand at 1966.553 kb on - strand at 1966.628 kb on + strand, within cheR at 1966.628 kb on + strand, within cheR at 1966.690 kb on - strand, within cheR at 1966.733 kb on + strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.890 kb on - strand, within cheR at 1967.084 kb on - strand, within cheR at 1967.202 kb on + strand, within cheR at 1967.413 kb on - strand at 1967.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,964,507 - cheZ b1881 0.14 +0.8 1,964,609 - cheZ b1881 0.30 +1.0 1,964,847 - cheZ b1881 0.67 +0.3 1,964,889 + cheZ b1881 0.73 -1.5 1,965,270 + cheY b1882 0.51 +0.8 1,965,696 - cheB b1883 0.21 -3.2 1,965,815 - cheB b1883 0.32 -1.2 1,966,252 + cheB b1883 0.74 -1.8 1,966,273 + cheB b1883 0.76 +1.5 1,966,356 - cheB b1883 0.84 -1.5 1,966,514 + +2.1 1,966,553 - +2.4 1,966,628 + cheR b1884 0.12 -2.0 1,966,628 + cheR b1884 0.12 +0.6 1,966,690 - cheR b1884 0.19 -1.0 1,966,733 + cheR b1884 0.24 -1.4 1,966,883 - cheR b1884 0.41 +0.9 1,966,883 - cheR b1884 0.41 +1.9 1,966,890 - cheR b1884 0.42 -0.9 1,967,084 - cheR b1884 0.65 -0.4 1,967,202 + cheR b1884 0.78 -1.9 1,967,413 - -1.0 1,967,413 - +1.1
Or see this region's nucleotide sequence