Strain Fitness in Escherichia coli BW25113 around b1451

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyncB and b1450 are separated by 197 nucleotidesb1450 and yncD are separated by 35 nucleotidesyncD and yncE are separated by 241 nucleotides b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088 yncB b1450: b1450 - orf, hypothetical protein (VIMSS), at 1,518,286 to 1,518,951 b1450 b1451: yncD - predicted iron outer membrane transporter (NCBI), at 1,518,987 to 1,521,089 yncD b1452: yncE - hypothetical protein (NCBI), at 1,521,331 to 1,522,392 yncE Position (kb) 1518 1519 1520 1521 1522Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1518.047 kb on - strandat 1518.065 kb on - strandat 1518.067 kb on - strandat 1518.147 kb on + strandat 1518.175 kb on - strandat 1518.175 kb on - strandat 1518.200 kb on + strandat 1518.225 kb on - strandat 1518.237 kb on + strandat 1518.254 kb on + strandat 1518.295 kb on - strandat 1518.330 kb on + strandat 1518.338 kb on - strandat 1518.349 kb on - strandat 1518.354 kb on + strand, within b1450at 1518.423 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.495 kb on + strand, within b1450at 1518.498 kb on + strand, within b1450at 1518.506 kb on + strand, within b1450at 1518.746 kb on - strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.767 kb on + strand, within b1450at 1518.775 kb on - strand, within b1450at 1518.779 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.871 kb on + strand, within b1450at 1518.872 kb on + strand, within b1450at 1518.954 kb on - strandat 1518.974 kb on + strandat 1519.053 kb on - strandat 1519.084 kb on - strandat 1519.089 kb on - strandat 1519.228 kb on - strand, within yncDat 1519.274 kb on - strand, within yncDat 1519.282 kb on - strand, within yncDat 1519.282 kb on - strand, within yncDat 1519.284 kb on - strand, within yncDat 1519.296 kb on + strand, within yncDat 1519.313 kb on + strand, within yncDat 1519.313 kb on + strand, within yncDat 1519.426 kb on + strand, within yncDat 1519.485 kb on - strand, within yncDat 1519.551 kb on - strand, within yncDat 1519.645 kb on + strand, within yncDat 1519.653 kb on - strand, within yncDat 1519.653 kb on - strand, within yncDat 1519.784 kb on - strand, within yncDat 1519.828 kb on + strand, within yncDat 1520.084 kb on - strand, within yncDat 1520.202 kb on + strand, within yncDat 1520.920 kb on - strandat 1520.920 kb on - strandat 1521.210 kb on - strandat 1521.495 kb on + strand, within yncEat 1521.525 kb on + strand, within yncEat 1521.525 kb on + strand, within yncEat 1521.556 kb on - strand, within yncEat 1521.593 kb on + strand, within yncEat 1521.593 kb on + strand, within yncEat 1521.642 kb on + strand, within yncEat 1521.660 kb on - strand, within yncEat 1521.660 kb on - strand, within yncEat 1521.932 kb on - strand, within yncEat 1522.035 kb on + strand, within yncEat 1522.067 kb on - strand, within yncE

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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1,518,047 - +1.7
1,518,065 - +1.7
1,518,067 - +1.7
1,518,147 + -1.3
1,518,175 - +1.7
1,518,175 - -0.6
1,518,200 + -0.1
1,518,225 - +0.7
1,518,237 + -0.9
1,518,254 + +0.3
1,518,295 - -0.6
1,518,330 + -0.5
1,518,338 - +1.7
1,518,349 - +1.7
1,518,354 + b1450 b1450 0.10 -1.1
1,518,423 + b1450 b1450 0.21 +1.5
1,518,425 + b1450 b1450 0.21 -1.4
1,518,425 + b1450 b1450 0.21 +2.9
1,518,425 + b1450 b1450 0.21 +1.7
1,518,425 + b1450 b1450 0.21 +0.1
1,518,495 + b1450 b1450 0.31 +1.5
1,518,498 + b1450 b1450 0.32 -0.4
1,518,506 + b1450 b1450 0.33 +0.4
1,518,746 - b1450 b1450 0.69 +1.7
1,518,756 + b1450 b1450 0.71 -1.1
1,518,756 + b1450 b1450 0.71 +3.4
1,518,767 + b1450 b1450 0.72 +0.3
1,518,775 - b1450 b1450 0.73 +1.7
1,518,779 + b1450 b1450 0.74 -0.1
1,518,844 + b1450 b1450 0.84 +0.7
1,518,844 + b1450 b1450 0.84 +0.1
1,518,871 + b1450 b1450 0.88 -0.6
1,518,872 + b1450 b1450 0.88 -0.7
1,518,954 - +0.1
1,518,974 + +0.7
1,519,053 - -2.0
1,519,084 - -1.6
1,519,089 - +0.7
1,519,228 - yncD b1451 0.11 -1.2
1,519,274 - yncD b1451 0.14 +0.7
1,519,282 - yncD b1451 0.14 -2.2
1,519,282 - yncD b1451 0.14 +2.8
1,519,284 - yncD b1451 0.14 +1.4
1,519,296 + yncD b1451 0.15 +1.7
1,519,313 + yncD b1451 0.16 +0.1
1,519,313 + yncD b1451 0.16 +1.7
1,519,426 + yncD b1451 0.21 +1.1
1,519,485 - yncD b1451 0.24 -2.1
1,519,551 - yncD b1451 0.27 -1.4
1,519,645 + yncD b1451 0.31 +1.5
1,519,653 - yncD b1451 0.32 -0.3
1,519,653 - yncD b1451 0.32 -0.5
1,519,784 - yncD b1451 0.38 +1.3
1,519,828 + yncD b1451 0.40 +2.2
1,520,084 - yncD b1451 0.52 +0.9
1,520,202 + yncD b1451 0.58 +0.1
1,520,920 - +1.5
1,520,920 - +0.4
1,521,210 - +0.7
1,521,495 + yncE b1452 0.15 -1.0
1,521,525 + yncE b1452 0.18 -0.6
1,521,525 + yncE b1452 0.18 +0.9
1,521,556 - yncE b1452 0.21 +3.5
1,521,593 + yncE b1452 0.25 -2.4
1,521,593 + yncE b1452 0.25 -1.3
1,521,642 + yncE b1452 0.29 -0.2
1,521,660 - yncE b1452 0.31 -0.1
1,521,660 - yncE b1452 0.31 -0.3
1,521,932 - yncE b1452 0.57 +0.3
1,522,035 + yncE b1452 0.66 +2.0
1,522,067 - yncE b1452 0.69 -0.3

Or see this region's nucleotide sequence