Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdoD and ydcH are separated by 139 nucleotides ydcH and rimL are separated by 62 nucleotides rimL and ydcK overlap by 9 nucleotides ydcK and tehA are separated by 123 nucleotides
b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535
mdoD
b1426: ydcH - orf, hypothetical protein (VIMSS), at 1,496,675 to 1,496,899
ydcH
b1427: rimL - ribosomal-protein-L7/L12-serine acetyltransferase (NCBI), at 1,496,962 to 1,497,501
rimL
b1428: ydcK - predicted enzyme (NCBI), at 1,497,493 to 1,498,473
ydcK
b1429: tehA - potassium-tellurite ethidium and proflavin transporter (NCBI), at 1,498,597 to 1,499,589
tehA
Position (kb)
1497
1498
1499 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1496.669 kb on - strand at 1496.669 kb on - strand at 1496.673 kb on - strand at 1496.808 kb on - strand, within ydcH at 1496.896 kb on - strand at 1497.142 kb on - strand, within rimL at 1497.231 kb on + strand, within rimL at 1497.266 kb on + strand, within rimL at 1497.328 kb on - strand, within rimL at 1497.407 kb on + strand, within rimL at 1497.636 kb on + strand, within ydcK at 1497.662 kb on + strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.765 kb on + strand, within ydcK at 1497.766 kb on + strand, within ydcK at 1497.792 kb on - strand, within ydcK at 1497.805 kb on + strand, within ydcK at 1497.829 kb on - strand, within ydcK at 1498.038 kb on + strand, within ydcK at 1498.172 kb on + strand, within ydcK at 1498.172 kb on + strand, within ydcK at 1498.189 kb on - strand, within ydcK at 1498.189 kb on - strand, within ydcK at 1498.219 kb on - strand, within ydcK at 1498.471 kb on - strand at 1498.779 kb on - strand, within tehA at 1499.017 kb on - strand, within tehA at 1499.151 kb on + strand, within tehA
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,496,669 - +0.1 1,496,669 - -1.3 1,496,673 - -0.7 1,496,808 - ydcH b1426 0.59 +0.3 1,496,896 - -1.9 1,497,142 - rimL b1427 0.33 -1.8 1,497,231 + rimL b1427 0.50 +1.7 1,497,266 + rimL b1427 0.56 +1.2 1,497,328 - rimL b1427 0.68 -0.1 1,497,407 + rimL b1427 0.82 +2.9 1,497,636 + ydcK b1428 0.15 +0.7 1,497,662 + ydcK b1428 0.17 +1.4 1,497,751 - ydcK b1428 0.26 -2.7 1,497,751 - ydcK b1428 0.26 +3.3 1,497,751 - ydcK b1428 0.26 +1.0 1,497,765 + ydcK b1428 0.28 +0.5 1,497,766 + ydcK b1428 0.28 -0.1 1,497,792 - ydcK b1428 0.30 -1.8 1,497,805 + ydcK b1428 0.32 +1.9 1,497,829 - ydcK b1428 0.34 -0.9 1,498,038 + ydcK b1428 0.56 +2.4 1,498,172 + ydcK b1428 0.69 +0.1 1,498,172 + ydcK b1428 0.69 +1.7 1,498,189 - ydcK b1428 0.71 +3.0 1,498,189 - ydcK b1428 0.71 -1.1 1,498,219 - ydcK b1428 0.74 +2.3 1,498,471 - +2.4 1,498,779 - tehA b1429 0.18 -0.3 1,499,017 - tehA b1429 0.42 +0.1 1,499,151 + tehA b1429 0.56 -0.9
Or see this region's nucleotide sequence