Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potA and pepT are separated by 249 nucleotides pepT and ycfD are separated by 48 nucleotides
b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817
potA
b1127: pepT - peptidase T (NCBI), at 1,185,067 to 1,186,293
pepT
b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463
ycfD
Position (kb)
1185
1186
1187 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1184.139 kb on - strand, within potA at 1184.163 kb on + strand, within potA at 1184.163 kb on + strand, within potA at 1184.286 kb on + strand, within potA at 1184.293 kb on + strand, within potA at 1184.334 kb on - strand, within potA at 1184.361 kb on - strand, within potA at 1184.599 kb on - strand, within potA at 1184.666 kb on + strand, within potA at 1184.692 kb on + strand, within potA at 1184.712 kb on + strand at 1184.724 kb on + strand at 1184.732 kb on - strand at 1184.847 kb on + strand at 1185.128 kb on + strand at 1185.135 kb on - strand at 1185.135 kb on - strand at 1185.153 kb on + strand at 1185.190 kb on + strand, within pepT at 1185.276 kb on + strand, within pepT at 1185.296 kb on + strand, within pepT at 1185.409 kb on + strand, within pepT at 1185.639 kb on + strand, within pepT at 1185.647 kb on + strand, within pepT at 1185.720 kb on + strand, within pepT at 1185.746 kb on - strand, within pepT at 1185.779 kb on - strand, within pepT at 1185.886 kb on + strand, within pepT at 1185.986 kb on - strand, within pepT at 1186.009 kb on + strand, within pepT at 1186.182 kb on - strand at 1186.468 kb on - strand, within ycfD at 1186.507 kb on - strand, within ycfD at 1186.533 kb on - strand, within ycfD at 1186.778 kb on + strand, within ycfD at 1186.778 kb on + strand, within ycfD at 1186.805 kb on - strand, within ycfD at 1186.876 kb on - strand, within ycfD at 1186.942 kb on - strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1187.202 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 1,184,139 - potA b1126 0.40 -2.2 1,184,163 + potA b1126 0.42 +0.9 1,184,163 + potA b1126 0.42 -2.5 1,184,286 + potA b1126 0.53 -2.4 1,184,293 + potA b1126 0.54 -1.4 1,184,334 - potA b1126 0.57 -3.9 1,184,361 - potA b1126 0.60 -0.1 1,184,599 - potA b1126 0.81 +1.9 1,184,666 + potA b1126 0.87 -3.4 1,184,692 + potA b1126 0.89 +0.2 1,184,712 + +0.3 1,184,724 + +0.3 1,184,732 - +3.2 1,184,847 + +0.6 1,185,128 + +0.8 1,185,135 - -2.1 1,185,135 - +0.2 1,185,153 + -4.0 1,185,190 + pepT b1127 0.10 +0.1 1,185,276 + pepT b1127 0.17 -2.0 1,185,296 + pepT b1127 0.19 -1.1 1,185,409 + pepT b1127 0.28 +0.3 1,185,639 + pepT b1127 0.47 -2.0 1,185,647 + pepT b1127 0.47 -2.2 1,185,720 + pepT b1127 0.53 -2.1 1,185,746 - pepT b1127 0.55 -0.4 1,185,779 - pepT b1127 0.58 +1.3 1,185,886 + pepT b1127 0.67 -0.8 1,185,986 - pepT b1127 0.75 -0.8 1,186,009 + pepT b1127 0.77 -0.5 1,186,182 - +2.1 1,186,468 - ycfD b1128 0.11 -2.6 1,186,507 - ycfD b1128 0.15 -0.6 1,186,533 - ycfD b1128 0.17 -4.1 1,186,778 + ycfD b1128 0.39 -2.0 1,186,778 + ycfD b1128 0.39 -2.7 1,186,805 - ycfD b1128 0.41 -0.7 1,186,876 - ycfD b1128 0.48 -2.0 1,186,942 - ycfD b1128 0.53 -1.2 1,186,950 + ycfD b1128 0.54 +0.5 1,186,950 + ycfD b1128 0.54 -0.2 1,187,202 + ycfD b1128 0.77 -2.3 1,187,292 + ycfD b1128 0.85 -2.1 1,187,292 + ycfD b1128 0.85 -2.6
Or see this region's nucleotide sequence