Strain Fitness in Escherichia coli BW25113 around b0685

Experiment: Ste41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfur and uof overlap by 8 nucleotidesuof and fldA are separated by 209 nucleotidesfldA and ybfE are separated by 139 nucleotidesybfE and ybfF are separated by 139 nucleotides b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869 fur b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948 uof b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688 fldA b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121 ybfE b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF Position (kb) 710 711 712Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 709.861 kb on - strandat 710.067 kb on - strandat 710.080 kb on + strandat 710.148 kb on - strandat 710.148 kb on - strandat 710.163 kb on - strandat 710.751 kb on + strandat 710.780 kb on - strandat 710.780 kb on - strandat 710.787 kb on + strandat 710.798 kb on + strandat 710.805 kb on - strandat 710.805 kb on - strandat 710.807 kb on + strandat 710.901 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 711.159 kb on - strandat 711.400 kb on - strand, within ybfFat 711.569 kb on + strand, within ybfFat 711.668 kb on - strand, within ybfFat 711.685 kb on + strand, within ybfFat 711.728 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.900 kb on - strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.933 kb on + strand, within ybfFat 712.060 kb on + strandat 712.077 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ste41
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709,861 - -3.1
710,067 - -0.2
710,080 + +0.3
710,148 - +0.1
710,148 - -1.7
710,163 - -3.6
710,751 + +2.8
710,780 - +2.5
710,780 - +0.6
710,787 + -2.6
710,798 + -1.3
710,805 - +1.1
710,805 - +2.1
710,807 + +2.1
710,901 + ybfE b0685 0.25 -1.9
710,924 + ybfE b0685 0.33 -1.7
710,924 + ybfE b0685 0.33 -0.1
711,159 - +0.5
711,400 - ybfF b0686 0.18 -0.5
711,569 + ybfF b0686 0.40 -0.8
711,668 - ybfF b0686 0.53 -0.3
711,685 + ybfF b0686 0.55 +1.5
711,728 - ybfF b0686 0.61 -3.2
711,730 - ybfF b0686 0.61 -2.7
711,730 - ybfF b0686 0.61 +0.7
711,803 - ybfF b0686 0.71 -2.0
711,803 - ybfF b0686 0.71 +0.2
711,900 - ybfF b0686 0.84 +0.8
711,923 + ybfF b0686 0.87 -0.6
711,923 + ybfF b0686 0.87 +0.9
711,933 + ybfF b0686 0.88 -1.1
712,060 + +0.7
712,077 + +1.7

Or see this region's nucleotide sequence