Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intF and ptwF are separated by 109 nucleotides ptwF and yagP are separated by 126 nucleotides yagP and ykgT overlap by 6 nucleotides ykgT and yagQ overlap by 22 nucleotides yagQ and yagR are separated by 9 nucleotides
b0281: intF - CP4-6 prophage; predicted phage integrase (NCBI), at 294,920 to 296,320
intF
b4629: ptwF - tRNA-OTHER (RefSeq), at 296,430 to 296,478
ptwF
b4694: yagP - no description, at 296,605 to 296,943
yagP
b4695: ykgT - no description, at 296,938 to 297,015
ykgT
b0283: yagQ - hypothetical protein (NCBI), at 296,994 to 297,950
yagQ
b0284: yagR - predicted oxidoreductase with molybdenum-binding domain (NCBI), at 297,960 to 300,158
yagR
Position (kb) 296
297
298 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 296.002 kb on + strand, within intF at 296.012 kb on + strand, within intF at 296.012 kb on + strand, within intF at 296.027 kb on + strand, within intF at 296.059 kb on + strand, within intF at 296.059 kb on + strand, within intF at 296.202 kb on - strand at 296.204 kb on - strand at 296.204 kb on - strand at 296.456 kb on - strand, within ptwF at 296.543 kb on + strand at 296.543 kb on + strand at 296.551 kb on - strand at 296.635 kb on - strand at 296.635 kb on - strand at 296.655 kb on + strand, within yagP at 296.813 kb on + strand, within yagP at 296.813 kb on + strand, within yagP at 296.818 kb on - strand, within yagP at 296.818 kb on - strand, within yagP at 296.896 kb on + strand, within yagP at 296.930 kb on + strand at 296.991 kb on + strand, within ykgT at 296.993 kb on - strand, within ykgT at 296.993 kb on - strand, within ykgT at 297.046 kb on - strand at 297.064 kb on + strand at 297.064 kb on + strand at 297.068 kb on + strand at 297.068 kb on + strand at 297.082 kb on - strand at 297.111 kb on - strand, within yagQ at 297.111 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.193 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.432 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.513 kb on + strand, within yagQ at 297.581 kb on + strand, within yagQ at 297.950 kb on - strand at 298.066 kb on + strand at 298.138 kb on - strand at 298.138 kb on - strand at 298.187 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.449 kb on + strand, within yagR at 298.449 kb on + strand, within yagR at 298.549 kb on - strand, within yagR at 298.581 kb on + strand, within yagR at 298.632 kb on + strand, within yagR at 298.728 kb on + strand, within yagR at 298.738 kb on + strand, within yagR at 298.770 kb on + strand, within yagR at 298.903 kb on + strand, within yagR at 298.906 kb on - strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 296,002 + intF b0281 0.77 -2.1 296,012 + intF b0281 0.78 +0.8 296,012 + intF b0281 0.78 -2.1 296,027 + intF b0281 0.79 -1.3 296,059 + intF b0281 0.81 +0.0 296,059 + intF b0281 0.81 +0.1 296,202 - -1.9 296,204 - +2.1 296,204 - -2.0 296,456 - ptwF b4629 0.53 +0.5 296,543 + +0.5 296,543 + -1.6 296,551 - -1.5 296,635 - -1.8 296,635 - -2.0 296,655 + yagP b4694 0.15 -3.7 296,813 + yagP b4694 0.61 -0.8 296,813 + yagP b4694 0.61 -2.4 296,818 - yagP b4694 0.63 -1.6 296,818 - yagP b4694 0.63 -2.7 296,896 + yagP b4694 0.86 +0.0 296,930 + +0.9 296,991 + ykgT b4695 0.68 +2.4 296,993 - ykgT b4695 0.71 +0.5 296,993 - ykgT b4695 0.71 +4.7 297,046 - -3.1 297,064 + +0.8 297,064 + +1.2 297,068 + -2.8 297,068 + +1.5 297,082 - +0.4 297,111 - yagQ b0283 0.12 +1.2 297,111 - yagQ b0283 0.12 -2.5 297,144 - yagQ b0283 0.16 -3.0 297,144 - yagQ b0283 0.16 +0.6 297,185 + yagQ b0283 0.20 -1.6 297,185 + yagQ b0283 0.20 +2.1 297,193 - yagQ b0283 0.21 +1.5 297,226 - yagQ b0283 0.24 +0.5 297,226 - yagQ b0283 0.24 -1.5 297,316 - yagQ b0283 0.34 -2.7 297,316 - yagQ b0283 0.34 +1.5 297,432 - yagQ b0283 0.46 +2.3 297,455 - yagQ b0283 0.48 -1.1 297,455 - yagQ b0283 0.48 +1.6 297,513 + yagQ b0283 0.54 -2.3 297,581 + yagQ b0283 0.61 +1.1 297,950 - +2.1 298,066 + +2.3 298,138 - -2.7 298,138 - -1.0 298,187 - yagR b0284 0.10 -0.1 298,267 - yagR b0284 0.14 +1.3 298,267 - yagR b0284 0.14 -1.6 298,449 + yagR b0284 0.22 +1.6 298,449 + yagR b0284 0.22 +2.1 298,549 - yagR b0284 0.27 -3.8 298,581 + yagR b0284 0.28 -1.5 298,632 + yagR b0284 0.31 -0.1 298,728 + yagR b0284 0.35 -2.8 298,738 + yagR b0284 0.35 +3.2 298,770 + yagR b0284 0.37 -3.5 298,903 + yagR b0284 0.43 +0.8 298,906 - yagR b0284 0.43 -2.2 298,908 + yagR b0284 0.43 -2.5 298,908 + yagR b0284 0.43 +2.9 298,908 + yagR b0284 0.43 -0.3
Or see this region's nucleotide sequence