Experiment: Ste41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadR and yadS are separated by 46 nucleotides yadS and btuF are separated by 37 nucleotides btuF and pfs overlap by 8 nucleotides pfs and dgt are separated by 83 nucleotides
b0156: yadR - hypothetical protein (NCBI), at 176,610 to 176,954
yadR
b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624
yadS
b0158: btuF - vitamin B12-transporter protein BtuF (NCBI), at 177,662 to 178,462
btuF
b0159: pfs - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI), at 178,455 to 179,153
pfs
b0160: dgt - deoxyguanosinetriphosphate triphosphohydrolase (NCBI), at 179,237 to 180,754
dgt
Position (kb) 177
178
179 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.002 kb on - strand at 177.019 kb on - strand at 177.019 kb on - strand at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.221 kb on - strand, within yadS at 177.257 kb on + strand, within yadS at 177.300 kb on - strand, within yadS at 177.300 kb on - strand, within yadS at 177.333 kb on + strand, within yadS at 177.341 kb on - strand, within yadS at 177.531 kb on + strand, within yadS at 177.578 kb on + strand at 177.596 kb on - strand at 177.596 kb on - strand at 177.616 kb on + strand at 177.616 kb on + strand at 177.625 kb on + strand at 177.625 kb on + strand at 177.697 kb on + strand at 177.714 kb on - strand at 177.726 kb on + strand at 177.735 kb on - strand at 178.094 kb on - strand, within btuF at 178.094 kb on - strand, within btuF at 178.138 kb on + strand, within btuF at 178.138 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.333 kb on + strand, within btuF at 178.440 kb on + strand at 178.440 kb on + strand at 178.448 kb on - strand at 178.452 kb on + strand at 179.187 kb on + strand at 179.228 kb on + strand at 179.291 kb on + strand at 179.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ste41 remove 177,002 - +1.2 177,019 - +0.8 177,019 - -1.9 177,084 - yadS b0157 0.13 -2.1 177,084 - yadS b0157 0.13 +2.3 177,084 - yadS b0157 0.13 +1.6 177,084 - yadS b0157 0.13 +1.1 177,084 - yadS b0157 0.13 +2.0 177,084 - yadS b0157 0.13 -0.5 177,221 - yadS b0157 0.35 -1.5 177,257 + yadS b0157 0.41 +2.3 177,300 - yadS b0157 0.48 +0.7 177,300 - yadS b0157 0.48 -0.0 177,333 + yadS b0157 0.53 +0.2 177,341 - yadS b0157 0.54 +1.0 177,531 + yadS b0157 0.85 -2.7 177,578 + -1.5 177,596 - -1.8 177,596 - -0.7 177,616 + -1.3 177,616 + +0.6 177,625 + +2.0 177,625 + +1.4 177,697 + +0.2 177,714 - +0.9 177,726 + +0.1 177,735 - -1.1 178,094 - btuF b0158 0.54 -1.3 178,094 - btuF b0158 0.54 +0.2 178,138 + btuF b0158 0.59 -0.8 178,138 - btuF b0158 0.59 -2.8 178,150 - btuF b0158 0.61 +0.4 178,150 - btuF b0158 0.61 +1.9 178,304 - btuF b0158 0.80 -2.8 178,304 - btuF b0158 0.80 +0.5 178,333 + btuF b0158 0.84 -0.8 178,440 + -0.9 178,440 + -1.3 178,448 - +1.8 178,452 + -1.1 179,187 + -0.2 179,228 + -2.0 179,291 + +0.2 179,378 + -0.2
Or see this region's nucleotide sequence