Experiment: Control_TnG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yicF and gmk are separated by 257 nucleotides gmk and rpoZ are separated by 54 nucleotides rpoZ and spoT are separated by 18 nucleotides
b3647: yicF - putative enzyme (VIMSS), at 3,817,511 to 3,819,193
yicF
b3648: gmk - guanylate kinase (NCBI), at 3,819,451 to 3,820,074
gmk
b3649: rpoZ - DNA-directed RNA polymerase subunit omega (NCBI), at 3,820,129 to 3,820,404
rpoZ
b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531
spoT
Position (kb)
3820
3821 Strain fitness (log2 ratio)
-2
-1
0
1 at 3819.130 kb on - strand at 3819.173 kb on - strand at 3819.191 kb on + strand at 3819.197 kb on - strand at 3819.197 kb on - strand at 3819.212 kb on - strand at 3819.234 kb on + strand at 3819.252 kb on - strand at 3819.252 kb on - strand at 3819.303 kb on - strand at 3819.394 kb on - strand at 3819.394 kb on - strand at 3820.087 kb on - strand at 3820.111 kb on - strand at 3820.144 kb on + strand at 3820.144 kb on + strand at 3820.151 kb on + strand at 3820.205 kb on + strand, within rpoZ at 3820.205 kb on + strand, within rpoZ at 3820.210 kb on + strand, within rpoZ at 3820.297 kb on + strand, within rpoZ at 3820.297 kb on + strand, within rpoZ at 3820.340 kb on + strand, within rpoZ at 3820.341 kb on - strand, within rpoZ at 3820.365 kb on + strand, within rpoZ at 3820.420 kb on + strand at 3820.420 kb on + strand at 3820.448 kb on - strand at 3820.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_TnG remove 3,819,130 - -0.1 3,819,173 - -0.1 3,819,191 + -0.2 3,819,197 - -0.2 3,819,197 - +0.2 3,819,212 - -0.8 3,819,234 + -0.2 3,819,252 - +0.7 3,819,252 - +0.1 3,819,303 - +0.3 3,819,394 - -0.7 3,819,394 - -0.1 3,820,087 - +0.1 3,820,111 - -0.4 3,820,144 + +0.4 3,820,144 + -1.3 3,820,151 + +0.7 3,820,205 + rpoZ b3649 0.28 +0.6 3,820,205 + rpoZ b3649 0.28 -2.3 3,820,210 + rpoZ b3649 0.29 -0.7 3,820,297 + rpoZ b3649 0.61 -2.0 3,820,297 + rpoZ b3649 0.61 +0.7 3,820,340 + rpoZ b3649 0.76 -0.7 3,820,341 - rpoZ b3649 0.77 +0.3 3,820,365 + rpoZ b3649 0.86 -0.5 3,820,420 + -1.2 3,820,420 + -0.0 3,820,448 - -0.4 3,820,452 + -0.1
Or see this region's nucleotide sequence