Experiment: Control_TnG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and secB overlap by 1 nucleotides secB and grxC are separated by 62 nucleotides grxC and yibN are separated by 141 nucleotides yibN and gpmI are separated by 244 nucleotides
b3608: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (NCBI), at 3,780,665 to 3,781,684
gpsA
b3609: secB - export protein SecB (NCBI), at 3,781,684 to 3,782,151
secB
b3610: grxC - glutaredoxin 3 (NCBI), at 3,782,214 to 3,782,465
grxC
b3611: yibN - predicted rhodanese-related sulfurtransferase (NCBI), at 3,782,607 to 3,783,038
yibN
b3612: gpmI - phosphoglyceromutase (NCBI), at 3,783,283 to 3,784,827
gpmI
Position (kb)
3782
3783
3784 Strain fitness (log2 ratio)
-1
0
1 at 3781.687 kb on - strand at 3781.716 kb on - strand at 3781.744 kb on - strand, within secB at 3781.790 kb on - strand, within secB at 3781.862 kb on - strand, within secB at 3781.890 kb on - strand, within secB at 3781.930 kb on - strand, within secB at 3781.930 kb on - strand, within secB at 3781.967 kb on - strand, within secB at 3781.980 kb on - strand, within secB at 3781.990 kb on - strand, within secB at 3781.990 kb on - strand, within secB at 3782.150 kb on - strand at 3782.150 kb on - strand at 3782.159 kb on - strand at 3782.185 kb on - strand at 3782.192 kb on + strand at 3782.195 kb on - strand at 3782.200 kb on - strand at 3782.200 kb on - strand at 3782.200 kb on - strand at 3782.220 kb on - strand at 3782.241 kb on - strand, within grxC at 3782.260 kb on - strand, within grxC at 3782.264 kb on - strand, within grxC at 3782.264 kb on - strand, within grxC at 3782.275 kb on + strand, within grxC at 3782.277 kb on + strand, within grxC at 3782.277 kb on + strand, within grxC at 3782.280 kb on + strand, within grxC at 3782.280 kb on + strand, within grxC at 3782.283 kb on - strand, within grxC at 3782.308 kb on + strand, within grxC at 3782.308 kb on + strand, within grxC at 3782.314 kb on - strand, within grxC at 3782.337 kb on + strand, within grxC at 3782.366 kb on - strand, within grxC at 3782.366 kb on - strand, within grxC at 3782.381 kb on + strand, within grxC at 3782.381 kb on + strand, within grxC at 3782.381 kb on - strand, within grxC at 3782.395 kb on + strand, within grxC at 3782.416 kb on - strand, within grxC at 3782.433 kb on + strand, within grxC at 3782.527 kb on + strand at 3782.527 kb on + strand at 3782.529 kb on + strand at 3782.535 kb on - strand at 3782.545 kb on - strand at 3782.561 kb on + strand at 3782.578 kb on + strand at 3782.589 kb on - strand at 3782.646 kb on + strand at 3782.656 kb on + strand, within yibN at 3782.708 kb on - strand, within yibN at 3782.709 kb on - strand, within yibN at 3782.726 kb on + strand, within yibN at 3782.726 kb on + strand, within yibN at 3782.726 kb on + strand, within yibN at 3782.729 kb on - strand, within yibN at 3782.729 kb on - strand, within yibN at 3782.729 kb on - strand, within yibN at 3782.766 kb on - strand, within yibN at 3782.769 kb on + strand, within yibN at 3782.769 kb on + strand, within yibN at 3782.787 kb on - strand, within yibN at 3782.854 kb on - strand, within yibN at 3782.858 kb on + strand, within yibN at 3782.892 kb on + strand, within yibN at 3782.892 kb on + strand, within yibN at 3782.999 kb on + strand at 3783.007 kb on - strand at 3783.092 kb on - strand at 3783.092 kb on - strand at 3783.107 kb on + strand at 3783.111 kb on - strand at 3783.111 kb on - strand at 3783.304 kb on + strand at 3783.326 kb on + strand at 3783.326 kb on + strand at 3783.346 kb on + strand at 3783.346 kb on - strand at 3783.386 kb on + strand at 3783.393 kb on + strand at 3783.393 kb on + strand at 3783.394 kb on + strand at 3783.406 kb on + strand at 3783.406 kb on + strand at 3783.444 kb on - strand, within gpmI at 3783.485 kb on + strand, within gpmI at 3783.485 kb on + strand, within gpmI at 3783.485 kb on + strand, within gpmI at 3783.490 kb on + strand, within gpmI at 3783.505 kb on - strand, within gpmI at 3783.511 kb on + strand, within gpmI at 3783.511 kb on + strand, within gpmI at 3783.527 kb on - strand, within gpmI at 3783.527 kb on - strand, within gpmI at 3783.528 kb on - strand, within gpmI at 3783.528 kb on - strand, within gpmI at 3783.537 kb on - strand, within gpmI at 3783.537 kb on - strand, within gpmI at 3783.619 kb on + strand, within gpmI at 3783.623 kb on + strand, within gpmI at 3783.623 kb on + strand, within gpmI at 3783.657 kb on - strand, within gpmI at 3783.667 kb on + strand, within gpmI at 3783.667 kb on + strand, within gpmI at 3783.667 kb on - strand, within gpmI at 3783.678 kb on + strand, within gpmI at 3783.678 kb on + strand, within gpmI at 3783.680 kb on - strand, within gpmI at 3783.680 kb on - strand, within gpmI at 3783.741 kb on - strand, within gpmI at 3783.764 kb on + strand, within gpmI at 3783.764 kb on + strand, within gpmI at 3783.764 kb on + strand, within gpmI at 3783.764 kb on + strand, within gpmI at 3783.764 kb on + strand, within gpmI at 3783.772 kb on - strand, within gpmI at 3783.811 kb on + strand, within gpmI at 3783.811 kb on + strand, within gpmI at 3783.836 kb on - strand, within gpmI at 3783.843 kb on + strand, within gpmI at 3783.929 kb on - strand at 3783.929 kb on - strand, within gpmI at 3783.948 kb on + strand, within gpmI at 3783.966 kb on - strand, within gpmI at 3783.966 kb on - strand, within gpmI at 3784.005 kb on - strand, within gpmI at 3784.005 kb on - strand, within gpmI at 3784.005 kb on - strand, within gpmI at 3784.009 kb on + strand, within gpmI
Per-strain Table
Position Strand Gene LocusTag Fraction Control_TnG remove 3,781,687 - -0.9 3,781,716 - +0.6 3,781,744 - secB b3609 0.13 -1.7 3,781,790 - secB b3609 0.23 +0.3 3,781,862 - secB b3609 0.38 +0.4 3,781,890 - secB b3609 0.44 -1.5 3,781,930 - secB b3609 0.53 +0.5 3,781,930 - secB b3609 0.53 -0.6 3,781,967 - secB b3609 0.60 -0.8 3,781,980 - secB b3609 0.63 +0.4 3,781,990 - secB b3609 0.65 +0.3 3,781,990 - secB b3609 0.65 -1.0 3,782,150 - -0.1 3,782,150 - -0.8 3,782,159 - +0.0 3,782,185 - +0.2 3,782,192 + +0.4 3,782,195 - +0.5 3,782,200 - +0.0 3,782,200 - +1.2 3,782,200 - +0.5 3,782,220 - +0.0 3,782,241 - grxC b3610 0.11 -0.6 3,782,260 - grxC b3610 0.18 +0.0 3,782,264 - grxC b3610 0.20 +0.0 3,782,264 - grxC b3610 0.20 -1.6 3,782,275 + grxC b3610 0.24 -0.6 3,782,277 + grxC b3610 0.25 +1.1 3,782,277 + grxC b3610 0.25 -1.0 3,782,280 + grxC b3610 0.26 -0.6 3,782,280 + grxC b3610 0.26 +1.0 3,782,283 - grxC b3610 0.27 +0.4 3,782,308 + grxC b3610 0.37 -0.5 3,782,308 + grxC b3610 0.37 -0.0 3,782,314 - grxC b3610 0.40 +1.1 3,782,337 + grxC b3610 0.49 +0.4 3,782,366 - grxC b3610 0.60 -0.8 3,782,366 - grxC b3610 0.60 -0.5 3,782,381 + grxC b3610 0.66 -1.4 3,782,381 + grxC b3610 0.66 -0.2 3,782,381 - grxC b3610 0.66 +0.6 3,782,395 + grxC b3610 0.72 +1.6 3,782,416 - grxC b3610 0.80 +0.0 3,782,433 + grxC b3610 0.87 +0.5 3,782,527 + -0.9 3,782,527 + -0.2 3,782,529 + -0.1 3,782,535 - -0.3 3,782,545 - -0.6 3,782,561 + +0.3 3,782,578 + -0.3 3,782,589 - -0.0 3,782,646 + +0.8 3,782,656 + yibN b3611 0.11 -1.5 3,782,708 - yibN b3611 0.23 -0.3 3,782,709 - yibN b3611 0.24 -0.4 3,782,726 + yibN b3611 0.28 -0.2 3,782,726 + yibN b3611 0.28 -0.7 3,782,726 + yibN b3611 0.28 -0.8 3,782,729 - yibN b3611 0.28 -0.6 3,782,729 - yibN b3611 0.28 -0.3 3,782,729 - yibN b3611 0.28 -0.6 3,782,766 - yibN b3611 0.37 -1.0 3,782,769 + yibN b3611 0.38 -0.2 3,782,769 + yibN b3611 0.38 -0.7 3,782,787 - yibN b3611 0.42 +0.8 3,782,854 - yibN b3611 0.57 -1.1 3,782,858 + yibN b3611 0.58 +0.2 3,782,892 + yibN b3611 0.66 +0.2 3,782,892 + yibN b3611 0.66 -1.7 3,782,999 + -0.0 3,783,007 - -0.6 3,783,092 - +0.2 3,783,092 - +0.4 3,783,107 + +0.7 3,783,111 - -0.1 3,783,111 - -0.4 3,783,304 + -0.3 3,783,326 + -0.4 3,783,326 + +0.3 3,783,346 + -0.2 3,783,346 - -0.1 3,783,386 + -0.2 3,783,393 + +1.3 3,783,393 + -0.2 3,783,394 + -0.5 3,783,406 + -0.4 3,783,406 + -0.4 3,783,444 - gpmI b3612 0.10 +0.9 3,783,485 + gpmI b3612 0.13 +0.9 3,783,485 + gpmI b3612 0.13 -0.6 3,783,485 + gpmI b3612 0.13 +0.8 3,783,490 + gpmI b3612 0.13 +0.0 3,783,505 - gpmI b3612 0.14 -0.3 3,783,511 + gpmI b3612 0.15 +0.3 3,783,511 + gpmI b3612 0.15 -0.3 3,783,527 - gpmI b3612 0.16 -0.3 3,783,527 - gpmI b3612 0.16 -0.6 3,783,528 - gpmI b3612 0.16 +0.2 3,783,528 - gpmI b3612 0.16 +0.0 3,783,537 - gpmI b3612 0.16 +0.7 3,783,537 - gpmI b3612 0.16 +0.5 3,783,619 + gpmI b3612 0.22 -1.0 3,783,623 + gpmI b3612 0.22 -0.8 3,783,623 + gpmI b3612 0.22 +0.1 3,783,657 - gpmI b3612 0.24 +0.1 3,783,667 + gpmI b3612 0.25 +0.2 3,783,667 + gpmI b3612 0.25 -0.2 3,783,667 - gpmI b3612 0.25 -0.2 3,783,678 + gpmI b3612 0.26 +0.3 3,783,678 + gpmI b3612 0.26 +0.3 3,783,680 - gpmI b3612 0.26 +0.1 3,783,680 - gpmI b3612 0.26 +0.4 3,783,741 - gpmI b3612 0.30 +0.5 3,783,764 + gpmI b3612 0.31 +0.1 3,783,764 + gpmI b3612 0.31 -0.7 3,783,764 + gpmI b3612 0.31 -0.0 3,783,764 + gpmI b3612 0.31 -1.2 3,783,764 + gpmI b3612 0.31 +0.3 3,783,772 - gpmI b3612 0.32 +0.3 3,783,811 + gpmI b3612 0.34 +0.2 3,783,811 + gpmI b3612 0.34 +0.1 3,783,836 - gpmI b3612 0.36 -0.7 3,783,843 + gpmI b3612 0.36 -0.5 3,783,929 - -0.4 3,783,929 - gpmI b3612 0.42 +1.2 3,783,948 + gpmI b3612 0.43 -0.1 3,783,966 - gpmI b3612 0.44 -0.1 3,783,966 - gpmI b3612 0.44 -0.1 3,784,005 - gpmI b3612 0.47 +0.6 3,784,005 - gpmI b3612 0.47 -0.4 3,784,005 - gpmI b3612 0.47 +0.1 3,784,009 + gpmI b3612 0.47 -0.4
Or see this region's nucleotide sequence