Experiment: Control_TnG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mazG and chpA are separated by 70 nucleotides chpA and chpR overlap by 1 nucleotides chpR and relA are separated by 77 nucleotides
b2781: mazG - nucleoside triphosphate pyrophosphohydrolase (NCBI), at 2,907,916 to 2,908,707
mazG
b2782: chpA - toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI), at 2,908,778 to 2,909,113
chpA
b2783: chpR - antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI), at 2,909,113 to 2,909,361
chpR
b2784: relA - (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI), at 2,909,439 to 2,911,673
relA
Position (kb)
2908
2909
2910 Strain fitness (log2 ratio)
-1
0
1 at 2907.991 kb on - strand at 2908.016 kb on - strand, within mazG at 2908.022 kb on - strand, within mazG at 2908.082 kb on + strand, within mazG at 2908.122 kb on - strand, within mazG at 2908.157 kb on - strand, within mazG at 2908.172 kb on + strand, within mazG at 2908.172 kb on + strand, within mazG at 2908.377 kb on - strand, within mazG at 2908.538 kb on + strand, within mazG at 2908.571 kb on + strand, within mazG at 2908.624 kb on + strand, within mazG at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.682 kb on - strand at 2908.754 kb on - strand at 2908.754 kb on - strand at 2908.764 kb on - strand at 2908.764 kb on - strand at 2908.772 kb on + strand at 2908.772 kb on + strand at 2908.854 kb on + strand, within chpA at 2908.991 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.253 kb on + strand, within chpR at 2909.258 kb on + strand, within chpR at 2909.311 kb on + strand, within chpR at 2909.487 kb on + strand at 2909.487 kb on + strand at 2909.490 kb on - strand at 2909.490 kb on - strand at 2909.556 kb on - strand at 2909.669 kb on - strand, within relA at 2909.776 kb on + strand, within relA at 2909.776 kb on + strand, within relA at 2910.051 kb on - strand, within relA at 2910.051 kb on - strand, within relA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_TnG remove 2,907,991 - +0.3 2,908,016 - mazG b2781 0.13 -0.1 2,908,022 - mazG b2781 0.13 -0.6 2,908,082 + mazG b2781 0.21 +0.3 2,908,122 - mazG b2781 0.26 -0.3 2,908,157 - mazG b2781 0.30 -0.7 2,908,172 + mazG b2781 0.32 +0.2 2,908,172 + mazG b2781 0.32 -0.4 2,908,377 - mazG b2781 0.58 -0.4 2,908,538 + mazG b2781 0.79 +0.7 2,908,571 + mazG b2781 0.83 +0.1 2,908,624 + mazG b2781 0.89 -0.1 2,908,674 + -1.0 2,908,674 + -1.1 2,908,674 + +0.6 2,908,674 + +0.1 2,908,682 - -0.7 2,908,754 - -0.2 2,908,754 - +0.1 2,908,764 - +0.5 2,908,764 - -1.0 2,908,772 + -0.1 2,908,772 + +0.5 2,908,854 + chpA b2782 0.23 -0.6 2,908,991 - chpA b2782 0.63 -0.6 2,909,017 - chpA b2782 0.71 +0.1 2,909,017 - chpA b2782 0.71 -0.8 2,909,032 + chpA b2782 0.76 -1.3 2,909,032 + chpA b2782 0.76 -0.3 2,909,253 + chpR b2783 0.56 +0.0 2,909,258 + chpR b2783 0.58 -0.1 2,909,311 + chpR b2783 0.80 -0.4 2,909,487 + +0.9 2,909,487 + -0.1 2,909,490 - +0.4 2,909,490 - +0.7 2,909,556 - +0.6 2,909,669 - relA b2784 0.10 +0.6 2,909,776 + relA b2784 0.15 +1.2 2,909,776 + relA b2784 0.15 -0.2 2,910,051 - relA b2784 0.27 +0.4 2,910,051 - relA b2784 0.27 -0.6
Or see this region's nucleotide sequence