Experiment: Control_TnG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt adhE and ychE are separated by 476 nucleotides ychE and oppA are separated by 737 nucleotides
b1241: adhE - fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI), at 1,294,669 to 1,297,344
adhE
b1242: ychE - predicted inner membrane protein (NCBI), at 1,297,821 to 1,298,468
ychE
b1243: oppA - oligopeptide transporter subunit (NCBI), at 1,299,206 to 1,300,837
oppA
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1296.997 kb on - strand, within adhE at 1297.301 kb on + strand at 1297.311 kb on - strand at 1297.349 kb on - strand at 1297.349 kb on - strand at 1297.391 kb on + strand at 1297.500 kb on + strand at 1297.500 kb on + strand at 1297.531 kb on - strand at 1297.531 kb on - strand at 1297.558 kb on - strand at 1297.750 kb on - strand at 1297.787 kb on - strand at 1297.936 kb on - strand, within ychE at 1298.207 kb on - strand, within ychE at 1298.242 kb on - strand, within ychE at 1298.242 kb on - strand, within ychE at 1298.286 kb on - strand, within ychE at 1298.300 kb on + strand, within ychE at 1298.334 kb on - strand, within ychE at 1298.383 kb on + strand, within ychE at 1298.706 kb on - strand at 1298.706 kb on - strand at 1298.778 kb on + strand at 1298.778 kb on + strand at 1298.844 kb on + strand at 1298.852 kb on + strand at 1298.937 kb on - strand at 1299.060 kb on + strand at 1299.129 kb on + strand at 1299.130 kb on - strand at 1299.130 kb on - strand at 1299.134 kb on + strand at 1299.139 kb on - strand at 1299.142 kb on - strand at 1299.172 kb on + strand at 1299.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_TnG remove 1,296,997 - adhE b1241 0.87 +0.6 1,297,301 + -0.3 1,297,311 - -0.9 1,297,349 - +0.3 1,297,349 - +0.1 1,297,391 + -0.1 1,297,500 + +0.5 1,297,500 + +0.7 1,297,531 - +1.5 1,297,531 - +0.4 1,297,558 - -0.4 1,297,750 - +0.2 1,297,787 - -0.2 1,297,936 - ychE b1242 0.18 -0.3 1,298,207 - ychE b1242 0.60 -0.6 1,298,242 - ychE b1242 0.65 -1.0 1,298,242 - ychE b1242 0.65 -0.4 1,298,286 - ychE b1242 0.72 -0.9 1,298,300 + ychE b1242 0.74 -1.8 1,298,334 - ychE b1242 0.79 -0.7 1,298,383 + ychE b1242 0.87 +0.3 1,298,706 - -0.3 1,298,706 - +0.6 1,298,778 + -0.7 1,298,778 + +0.2 1,298,844 + +0.5 1,298,852 + +0.1 1,298,937 - -0.0 1,299,060 + -0.6 1,299,129 + +0.4 1,299,130 - -0.5 1,299,130 - +3.0 1,299,134 + -0.0 1,299,139 - -0.2 1,299,142 - +1.0 1,299,172 + +0.1 1,299,290 + -0.0
Or see this region's nucleotide sequence