Experiment: Control_TnG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt treA and dhaM are separated by 319 nucleotides dhaM and dhaL are separated by 10 nucleotides dhaL and b1200 are separated by 10 nucleotides
b1197: treA - periplasmic trehalase (NCBI), at 1,244,902 to 1,246,599
treA
b1198: dhaM - fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component (RefSeq), at 1,246,919 to 1,248,337
dhaM
b1199: dhaL - dihydroxyacetone kinase, C-terminal domain (NCBI), at 1,248,348 to 1,248,980
dhaL
b1200: b1200 - putative dihydroxyacetone kinase (EC 2.7.1.2) (VIMSS), at 1,248,991 to 1,250,061
b1200
Position (kb)
1246
1247
1248
1249 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1245.952 kb on + strand, within treA at 1245.952 kb on + strand, within treA at 1246.107 kb on - strand, within treA at 1246.158 kb on + strand, within treA at 1246.160 kb on + strand, within treA at 1246.160 kb on + strand, within treA at 1246.168 kb on - strand, within treA at 1246.185 kb on - strand, within treA at 1246.410 kb on - strand, within treA at 1246.505 kb on - strand at 1246.513 kb on - strand at 1246.666 kb on - strand at 1246.696 kb on - strand at 1246.799 kb on - strand at 1246.799 kb on - strand at 1246.898 kb on - strand at 1246.904 kb on + strand at 1246.924 kb on + strand at 1246.925 kb on - strand at 1246.944 kb on + strand at 1246.944 kb on + strand at 1247.303 kb on + strand, within dhaM at 1247.303 kb on + strand, within dhaM at 1247.349 kb on + strand, within dhaM at 1247.376 kb on + strand, within dhaM at 1247.377 kb on + strand, within dhaM at 1247.377 kb on - strand, within dhaM at 1247.497 kb on - strand, within dhaM at 1247.566 kb on - strand, within dhaM at 1247.604 kb on - strand, within dhaM at 1247.664 kb on - strand, within dhaM at 1247.683 kb on + strand, within dhaM at 1247.683 kb on + strand, within dhaM at 1247.892 kb on + strand, within dhaM at 1247.923 kb on + strand, within dhaM at 1247.932 kb on - strand, within dhaM at 1247.938 kb on + strand, within dhaM at 1248.025 kb on + strand, within dhaM at 1248.120 kb on + strand, within dhaM at 1248.131 kb on - strand, within dhaM at 1248.142 kb on + strand, within dhaM at 1248.251 kb on + strand at 1248.251 kb on + strand at 1248.305 kb on + strand at 1248.472 kb on + strand, within dhaL at 1248.497 kb on - strand, within dhaL at 1248.512 kb on - strand, within dhaL at 1248.571 kb on + strand, within dhaL at 1248.907 kb on - strand, within dhaL at 1248.907 kb on - strand, within dhaL at 1248.967 kb on + strand at 1249.100 kb on + strand, within b1200 at 1249.100 kb on + strand, within b1200 at 1249.132 kb on + strand, within b1200 at 1249.317 kb on + strand, within b1200
Per-strain Table
Position Strand Gene LocusTag Fraction Control_TnG remove 1,245,952 + treA b1197 0.62 -0.7 1,245,952 + treA b1197 0.62 -0.2 1,246,107 - treA b1197 0.71 -1.4 1,246,158 + treA b1197 0.74 -0.4 1,246,160 + treA b1197 0.74 -0.6 1,246,160 + treA b1197 0.74 -0.2 1,246,168 - treA b1197 0.75 -1.2 1,246,185 - treA b1197 0.76 -0.6 1,246,410 - treA b1197 0.89 +0.9 1,246,505 - +1.7 1,246,513 - +0.9 1,246,666 - +0.2 1,246,696 - -0.6 1,246,799 - +0.7 1,246,799 - +0.1 1,246,898 - -0.1 1,246,904 + -0.6 1,246,924 + +2.9 1,246,925 - -0.5 1,246,944 + +0.5 1,246,944 + -0.1 1,247,303 + dhaM b1198 0.27 -2.1 1,247,303 + dhaM b1198 0.27 -1.6 1,247,349 + dhaM b1198 0.30 +1.3 1,247,376 + dhaM b1198 0.32 -1.5 1,247,377 + dhaM b1198 0.32 -0.6 1,247,377 - dhaM b1198 0.32 -1.4 1,247,497 - dhaM b1198 0.41 -1.0 1,247,566 - dhaM b1198 0.46 -0.4 1,247,604 - dhaM b1198 0.48 +0.3 1,247,664 - dhaM b1198 0.53 +0.1 1,247,683 + dhaM b1198 0.54 -0.3 1,247,683 + dhaM b1198 0.54 -0.1 1,247,892 + dhaM b1198 0.69 -1.8 1,247,923 + dhaM b1198 0.71 -0.2 1,247,932 - dhaM b1198 0.71 -1.3 1,247,938 + dhaM b1198 0.72 -0.9 1,248,025 + dhaM b1198 0.78 -0.4 1,248,120 + dhaM b1198 0.85 +0.2 1,248,131 - dhaM b1198 0.85 -1.2 1,248,142 + dhaM b1198 0.86 +1.2 1,248,251 + +0.8 1,248,251 + -2.1 1,248,305 + -0.3 1,248,472 + dhaL b1199 0.20 -0.7 1,248,497 - dhaL b1199 0.24 -0.1 1,248,512 - dhaL b1199 0.26 +0.0 1,248,571 + dhaL b1199 0.35 +0.1 1,248,907 - dhaL b1199 0.88 -0.2 1,248,907 - dhaL b1199 0.88 +0.1 1,248,967 + -0.6 1,249,100 + b1200 b1200 0.10 +0.2 1,249,100 + b1200 b1200 0.10 -1.6 1,249,132 + b1200 b1200 0.13 -1.2 1,249,317 + b1200 b1200 0.30 -0.3
Or see this region's nucleotide sequence