Strain Fitness in Escherichia coli BW25113 around b1298

Experiment: Laval1267

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1297 and ycjL are separated by 211 nucleotidesycjL and puuR are separated by 26 nucleotidespuuR and puuC are separated by 274 nucleotides b1297: b1297 - putative glutamine synthetase (EC 6.3.1.2) (VIMSS), at 1,357,514 to 1,358,932 b1297 b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908 ycjL b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492 puuR b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254 puuC Position (kb) 1359 1360Strain fitness (log2 ratio) -1 0 1at 1358.414 kb on + strand, within b1297at 1358.462 kb on + strand, within b1297at 1358.477 kb on - strand, within b1297at 1358.559 kb on + strand, within b1297at 1358.640 kb on - strand, within b1297at 1358.640 kb on - strand, within b1297at 1358.860 kb on + strandat 1358.868 kb on - strandat 1359.236 kb on + strand, within ycjLat 1359.236 kb on + strand, within ycjLat 1359.453 kb on + strand, within ycjLat 1359.594 kb on + strand, within ycjLat 1359.759 kb on - strand, within ycjLat 1360.081 kb on - strand, within puuRat 1360.084 kb on - strand, within puuRat 1360.317 kb on - strand, within puuRat 1360.332 kb on - strand, within puuRat 1360.334 kb on + strand, within puuRat 1360.413 kb on - strand, within puuRat 1360.478 kb on - strandat 1360.478 kb on - strandat 1360.521 kb on - strandat 1360.526 kb on - strandat 1360.526 kb on - strandat 1360.698 kb on - strandat 1360.698 kb on - strandat 1360.761 kb on - strandat 1360.903 kb on - strandat 1360.903 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1267
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1,358,414 + b1297 b1297 0.63 -0.6
1,358,462 + b1297 b1297 0.67 -0.0
1,358,477 - b1297 b1297 0.68 +0.0
1,358,559 + b1297 b1297 0.74 +0.1
1,358,640 - b1297 b1297 0.79 -0.7
1,358,640 - b1297 b1297 0.79 -0.6
1,358,860 + +1.0
1,358,868 - -0.8
1,359,236 + ycjL b1298 0.12 +0.0
1,359,236 + ycjL b1298 0.12 -0.3
1,359,453 + ycjL b1298 0.40 -0.6
1,359,594 + ycjL b1298 0.59 -0.5
1,359,759 - ycjL b1298 0.80 -1.0
1,360,081 - puuR b1299 0.26 -0.1
1,360,084 - puuR b1299 0.27 +0.1
1,360,317 - puuR b1299 0.68 -0.3
1,360,332 - puuR b1299 0.71 +0.7
1,360,334 + puuR b1299 0.72 -0.8
1,360,413 - puuR b1299 0.86 -0.1
1,360,478 - -0.9
1,360,478 - +0.1
1,360,521 - +0.1
1,360,526 - +1.7
1,360,526 - +0.8
1,360,698 - -0.7
1,360,698 - -0.7
1,360,761 - +0.6
1,360,903 - +1.7
1,360,903 - +0.1

Or see this region's nucleotide sequence